Rhof (NM_175092) Mouse Untagged Clone

CAT#: MC207452

Rhof (untagged) - Mouse ras homolog gene family, member f (Rhof), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rhof
• Synonyms: AI845056; Arhf; AV026554; Ifld1; Rif
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207452 representing NM_175092
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCCCGGGTGCTCCGGCCCCAGCCGCCGCCCCCAGCTCGGCGAGGAAGGAGCTGAAGATAGTGA
  TCGTAGGTGACGGCGGCTGCGGCAAGACGTCCCTGCTAATGGTGTACTGCCAAGGCTCATTTCCCGAGCA
  CTACGCCCCGTCGGTGTTTGAGAAGTACACGGCTAGTGTGACAGTGGGTAACAAGGAGGTGACCCTGAAC
  CTCTACGACACGGCTGGGCAGGAAGACTACGATAGGCTGCGGCCCTTGTCCTACCAGAACACACACCTCG
  TCCTCATCTGCTACGATGTCATGAACCCCACCAGTTACGACAACGTCCTCATCAAGTGGTTCCCCGAAGT
  CACACATTTCTGCCGAGGGATCCCCACGGTGCTCATCGGCTGCAAGACAGACCTGAGGAAGGACAAGGAG
  CAGTTGCGGAAACTCCGGGCAGCTCAGCTGGAGCCTATTACCTACACACAGGGCCTGAATGCCTGTGAAC
  AGATGCGAGGTGCACTCTATCTGGAATGTTCTGCCAAGTTTCGGGAGAATGTGGAAGATGTCTTCAGGGA
  AGCCGCCAAGGTGGCCCTCAGCGCCCTGAAGAAAGCGCAGAGACAGAAAAAACACAGGATCTGCCTGCTG
  CTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175092
• Insert Size: 636 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175092.3, NP_780301.1
• RefSeq Size: 2143 bp
• RefSeq ORF: 636 bp
• Locus ID: 23912
• UniProt ID: Q8BYP3
• Cytogenetics: 5 62.77 cM
• Gene Summary: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology (By similarity).[UniProtKB/Swiss-Prot Function]