Vasp (NM_009499) Mouse Untagged Clone

CAT#: MC207439

Vasp (untagged) - Mouse vasodilator-stimulated phosphoprotein (Vasp), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vasp
• Synonyms: AA107290
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207439 representing NM_009499
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGACGGTCATCTGTTCCAGCCGGGCTACTGTGATGCTTTATGATGACAGCAACAAGCGATGGC
  TCCCTGCTGGCACTGGTCCCCAGGCCTTCAGCCGCGTCCAGATCTACCACAACCCCACTGCTAACTCCTT
  CCGAGTTGTTGGCCGCAAGATGCAGCCGGATCAGCAGGTGGTTATCAACTGTGCCATCATTCGGGGTGTC
  AAGTACAATCAGGCCACTCCCATCTTCCATCAGTGGCGAGATGCCCGCCAGGTCTGGGGCCTCAACTTCG
  GCAGCAAGGAGGACGCCATACAGTTTGCAACAGGCATGGCCAACGCCCTAGAGGCCTTGGAAGGAGGTGG
  GCCTCCCCCAGCCCCAGCACCCCCTGCCTGGTCTGCCCAGAATGGTCCCTCCCCAGAGGAGCTGGAACAA
  CAGAAAAGGCAGCCGGAGCATATGGAGCGCCGGGTCTCCAATGCAGGAGGCCCACCTGCTCCCCCAGCTG
  GGGGCCCTCCTCCACCTCCAGGACCTCCCCCTCCTCCAGGTCCCCCCCCACCCCCAGGTCTGCCCTCCTC
  AGGGGTATCTGGGGCAGGTCATGGAGCAGGGGCAGCCCCACCCCCTGCACCCCCACTCCCTACAGCACAG
  GGCCCCAATAGTGGGGGTTCCGGGGCCCCAGGCCTGGCTGCTGCCATTGCTGGAGCCAAACTCAGGAAAG
  TGAGCAAGCAGGAGGAGGCCTCTGGGGGGCCCCTGGCCCCCAAAGCTGAGAACAGTCGAAGCACTGGTGG
  GGGGCTTATGGAAGAGATGAACGCCATGCTGGCCCGGAGAAGAAAAGCCACACAGGTTGGGGAGAAGCCC
  CCCAAAGACGAGTCAGCCAGTGAGGAGTCAGAGGCCCGACTCCCTGCCCAGAGTGAACCTGTGAGAAGAC
  CCTGGGAGAAGAACAGCACAACCTTGCCAAGGATGAAGTCGTCCTCTTCTGTGACTACCTCCGAGGCCCA
  CCCCTCGACGCCCTGCTCCAGTGATGACTCCGACTTGGAGAGGGTGAAGCAGGAGCTTCTGGAAGAGGTG
  CGGAAGGAGCTACAGAAAATGAAAGAGGAAATCATCGAAGTCTTTGTCCAGGAGCTGAGGAAGCGGGGTT
  CTCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009499
• Insert Size: 1128 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009499.3, NP_033525.2
• RefSeq Size: 2267 bp
• RefSeq ORF: 1128 bp
• Locus ID: 22323
• UniProt ID: P70460
• Cytogenetics: 7 A3
• Gene Summary: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).