Usp12 (NM_011669) Mouse Untagged Clone

CAT#: MC207436

Usp12 (untagged) - Mouse ubiquitin specific peptidase 12 (Usp12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp12
• Synonyms: Ubh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207436 representing NM_011669
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCCTAATGACAGTCTCCAAATTCGCCTCCATCTGTACCATGGGCGCCAATGCCTCGGCATTAG
  AGAAAGAGATTGGTCCAGAACAGTTTCCGGTCAACGAGCACTATTTTGGATTAGTCAATTTTGGGAATAC
  CTGCTACTGCAACTCAGTTCTTCAAGCACTTTATTTTTGTCGTCCATTTCGGGAAAAAGTTCTAGCATAC
  AAAAGTCAGCCCAGAAAAAAGGAGAACCTTCTCACCTGCCTGGCAGACCTCTTCCACAGCATAGCCACCC
  AGAAGAAGAAGGTCGGCGTGATCCCGCCCAAGAAGTTCATCACAAGGTTGCGGAAAGAAAACGAGCTGTT
  TGACAACTACATGCAACAAGATGCCCATGAATTCTTAAATTACCTACTCAATACAATTGCTGATATTCTA
  CAAGAAGAAAGAAAACAGGAGAAGCAAAATGGCCGATTACGGAACGGAGACGTGGACAACGAGGATAACA
  ACAGCACACCAGACCCAACCTGGGTCCACGAGATCTTTCAGGGAACATTAACTAATGAAACCAGGTGTCT
  TACTTGTGAAACTATAAGCAGCAAAGATGAAGATTTTTTAGACCTTTCTGTTGACGTGGAACAAAACACA
  TCAATTACTCACTGCTTAAGAGGGTTCAGTAACACAGAAACTCTGTGCAGTGAATATAAGTACTACTGTG
  AAGAGTGCCGGAGCAAGCAGGAAGCACACAAGCGGATGAAGGTTAAGAAACTGCCCCTGATCCTGGCTCT
  GCACCTGAAGAGGTTTAAGTATATGGACCAGCTTCACCGGTACACGAAGCTTTCTTATCGCGTGGTCTTT
  CCTTTAGAGCTCCGTCTGTTCAACACGTCAGGAGATGCGACCAACCCTGACAGAATGTATGACCTGGTCG
  CCGTTGTGGTTCACTGTGGAAGTGGTCCCAACCGAGGCCATTATATTGCAATAGTTAAGAGTCATGATTT
  CTGGCTGTTGTTTGATGATGACATTGTAGAAAAAATCGATGCCCAAGCTATTGAAGAATTCTACGGATTG
  ACATCTGACATCTCCAAGAACTCGGAGTCTGGCTACATCCTTTTCTATCAGTCTCGGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011669
• Insert Size: 1113 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011669.3, NP_035799.1
• RefSeq Size: 4274 bp
• RefSeq ORF: 1113 bp
• Locus ID: 22217
• UniProt ID: Q9D9M2
• Cytogenetics: 5 G3
• Gene Summary: Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.[UniProtKB/Swiss-Prot Function]