Prkx (NM_016979) Mouse Untagged Clone

CAT#: MC207359

Prkx (untagged) - Mouse protein kinase, X-linked (Prkx), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prkx
• Synonyms: Pkare; PKX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207359 representing NM_016979
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCCCGCTGGTGCCGCGGCGACCGTAAAAGATCCAGACCATGACCCCGTGAAGACCAAGGTCT
  CGGCGCCCGCCGCGGACCCCAAACCCAGGACATCGTCACAGAAGGCGGGGCACTCGTTACAAGATTGGGA
  CACCATAGCCACCGTGGGCACTGGGACCTTTGGCCGTGTAAACCTGGTGAAGGAGAAGACAGGCAGGCAA
  TACTGTGCCTTGAAGATTATGAGTATCCCAGATGTCATCCGCCTGAAACAGGAACAGCATGTGCAGAATG
  AGAAAGCAGTCCTGAAGGAAATAAACCATCCCTTCCTCATTAAACTGCTTTGGACAGGCCATGACAACCG
  CTTCCTATACATGTTGATGGAGTTCGTGCCTGGTGGTGAGCTCTTCACCTACCTGCGTAATCGTGGCCGA
  TTCTCCTCAGTTGCTTCAGTCTTCTACGCCACAGAGATTGTGTGTGCCATAGAATATCTACACTCAAAGG
  AAATCGTATACAGGGACCTGAAGCCTGAAAACATCCTTCTAGACAGGGAAGGACATATTAAACTGACAGA
  CTTCGGCTTCGCCAAGAAACTGGTGGATAGGACATGGACTCTCTGTGGGACACCAGAATATCTCGCCCCA
  GAAGTCATTCAGAGCAAAGGTCATGGAAGGGCTGTGGACTGGTGGGCACTGGGCATTCTTATATTCGAGA
  TGCTGTCTGGGTTTCCCCCATTTTTCGATGACAACCCGTTTGGGATTTACCAGAAAATCCTTGCATGCAA
  AATAGATTTCCCCAGACAATTGGATTTCACCTCTAAGGACCTCATCAAGAAGCTGCTGGTGGTGGACAGG
  ACCCGACGCCTAGGCAACATGAAGAATGGGGCAGAAGACATCAAGCGGCACCGGTGGTTCCGAGGTGTGG
  AGTGGGAATCTGTGCCACAAAGAAAACTGAAGCCACCCATTGTGCCCAAGTTATCGGGTGATGGTGACAT
  CTCCAACTTTGAGACTTATCCAGAGAGTGAATTGGACAAGACACCTTCTGTATCTGACAAAGACCTGGAA
  ACATTCAAAAATTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016979
• Insert Size: 1068 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016979.1, NP_058675.1
• RefSeq Size: 3217 bp
• RefSeq ORF: 1068 bp
• Locus ID: 19108
• UniProt ID: Q922R0
• Cytogenetics: X B
• Gene Summary: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.[UniProtKB/Swiss-Prot Function]