Gja4 (NM_008120) Mouse Untagged Clone

CAT#: MC207286

Gja4 (untagged) - Mouse gap junction protein, alpha 4 (Gja4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gja4
• Synonyms: AU020209; AW558810; Cnx37; Cx37; Gja-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207286 representing NM_008120
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGGGCTTCCTGGAGAAGTTGCTAGACCAGGTCCAGGAACACTCGACCGTGGTGGGCAAGA
  TCTGGTTAACGGTGCTCTTCATCTTCCGCATCCTCATCCTGGGGCTGGCTGGCGAGTCGGTGTGGGGCGA
  CGAGCAGTCTGATTTTGAGTGTAACACAGCCCAGCCGGGCTGCACCAACGTCTGCTATGACCAGGCCTTC
  CCCATCTCCCACATCCGATACTGGGTGCTGCAGTTCCTCTTCGTCAGCACACCCACCCTGATCTACCTGG
  GCCATGTCATCTACCTGTCTCGGCGGGAAGAGCGGTTGCGGCAGAAAGAGGGAGAGCTCCGGGCGCTGCC
  ATCCAAGGACCTACATGTAGAGCGGGCACTGGCTGCCATCGAACATCAGATGGCCAAGATCTCGGTGGCA
  GAGGACGGTCGTCTTCGGATTCGTGGGGCGCTCATGGGTACCTATGTGGTCAGCGTGCTGTGTAAGAGTG
  TGCTGGAGGCAGGCTTCCTCTATGGCCAGTGGCGCCTCTATGGCTGGACCATGGAGCCGGTGTTTGTGTG
  CCAGCGTGCACCCTGCCCCCACATCGTGGACTGCTATGTCTCTCGACCCACTGAGAAGACTATCTTCATC
  ATCTTCATGCTGGTGGTAGGAGTCATCTCCCTGGTGCTCAACCTGCTGGAGCTGGTTCACCTGCTGTGTC
  GGTGTGTCAGCCGGGAGATAAAGGCACGAAGGGACCAGGACGCCCGCCCGGCCCAGGGCAGTGCCTCAGA
  CCCTTACCCTGAACAGGTTTTCTTCTACCTCCCCATGGGCGAGGGACCCTCTTCCCCACCGTGTCCCACC
  TACAACGGGCTCTCATCCACTGAGCAGAACTGGGCCAACTTGACCACAGAGGAGAGACTGACCTCTTCCA
  GACCTCCCCCATTTGTAAACACAGCTCCCCAGGGTGGCCGAAAGTCCCCTAGCCGCCCCAACAGCTCTGC
  ATCCAAGAAGCAGTATGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008120
• Insert Size: 1002 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008120.3, NP_032146.1
• RefSeq Size: 1686 bp
• RefSeq ORF: 1002 bp
• Locus ID: 14612
• UniProt ID: P28235
• Cytogenetics: 4 61.47 cM
• Gene Summary: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.[UniProtKB/Swiss-Prot Function]