Fbl (NM_007991) Mouse Untagged Clone

CAT#: MC207271

Fbl (untagged) - Mouse fibrillarin (Fbl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbl
• Synonyms: AL022665; FIB; FLRN; RNU3IP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207271 representing NM_007991
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCAGGTTTCAGCCCCCGTGGGGGTGGCTTTGGTGGCAGAGGCGGCTTTGGTGACAGAGGCGGTA
  GAGGAGGTGGAAGAGGAGGCCGAGGAGGCTTTGGCGGTGGACGAGGAGGCTTTGGCGGTGGAGGTCGAGG
  TCGAGGCGGAGGGGGTGGTGGCTTCAGGGGACGAGGAGGTGGCGGTGGCCGAGGTGGGGGCTTCCAGTCT
  GGGGGCAACCGGGGTCGAGGTGGTGGCCGGGGAGGCAAGAGAGGAAACCAGTCAGGGAAGAATGTGATGG
  TGGAGCCGCATCGTCATGAAGGTGTCTTTATCTGTCGCGGAAAGGAGGATGCCCTTGTCACAAAGAATCT
  GGTCCCTGGAGAGTCTGTGTATGGAGAGAAGAGAGTCTCTATTTCAGAAGGAGATGACAAAATTGAGTAC
  AGAGCCTGGAACCCCTTCCGCTCCAAGCTGGCAGCAGCTATCCTGGGCGGCGTAGACCAGATCCACATCA
  AGCCAGGAGCCAAGGTGCTCTACCTTGGGGCAGCCTCAGGCACCACCGTCTCCCACGTCTCTGATATTGT
  CGGCCCGGATGGTCTGGTCTACGCAGTTGAGTTCTCCCACCGCTCTGGCCGTGACCTCATCAACTTGGCC
  AAGAAGAGGACTAACATTATTCCTGTAATTGAAGATGCTCGACACCCACACAAATACCGCATGCTTATCG
  CAATGGTGGATGTCATCTTTGCCGATGTGGCCCAGCCAGACCAAACCCGAATTGTGGCCCTGAACGCCCA
  CACCTTCCTGCGGAATGGAGGACACTTTGTGATTTCCATTAAGGCCAACTGCATTGACTCCACTGCGTCA
  GCAGAGGCTGTGTTTGCATCTGAAGTGAAGAAGATGCAGCAGGAGAACATGAAGCCGCAGGAGCAGCTGA
  CGCTAGAGCCTTATGAGCGAGACCACGCCGTGGTTGTCGGTGTGTACAGGCCACCTCCCAAGGTGAAGAA
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007991
• Insert Size: 984 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007991.3, NP_032017.2
• RefSeq Size: 1159 bp
• RefSeq ORF: 984 bp
• Locus ID: 14113
• UniProt ID: P35550
• Cytogenetics: 7 A3
• Gene Summary: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).[UniProtKB/Swiss-Prot Function]