S1pr3 (NM_010101) Mouse Untagged Clone

CAT#: MC207261

S1pr3 (untagged) - Mouse sphingosine-1-phosphate receptor 3 (S1pr3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: S1pr3
• Synonyms: AI132464; Edg3; LP; Lpb3; S1; S1p3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207261 representing NM_010101
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCACGCATGCGCAGGGCCACCAGCCAGTCTTGGGAAATGACACTCTCCGGGAACATTACGATT
  ACGTGGGGAAACTGGCAGGCAGGCTGCGGGACCCCCCTGAGGGTGGCACCCTCATCACCACCATCCTCTT
  CTTGGTCACCTGTAGCTTCATCGTCTTGGAGAACCTGATGGTTTTGATTGCCATCTGGAAAAACAATAAA
  TTTCACAACCGCATGTACTTTTTCATCGGCAACTTGGCTCTCTGCGACCTGCTGGCCGGCATAGCATACA
  AGGTCAATATTCTTATGTCCGGCAGGAAGACGTTCAGTCTGTCTCCAACAGTGTGGTTCCTCAGGGAGGG
  CAGTATGTTCGTAGCCCTGGGCGCATCCACCTGCAGCTTACTGGCCATTGCCATTGAGCGACACCTGACC
  ATGATCAAGATGAGGCCATATGATGCCAACAAGAAGCACCGCGTGTTCCTTCTGATTGGGATGTGCTGGC
  TAATTGCCTTCTCGCTGGGTGCCCTGCCAATCCTGGGCTGGAACTGCCTGGAGAACTTTCCCGACTGCTC
  TACCATCTTGCCCCTCTACTCCAAGAAATACATCGCCTTCCTCATCAGTATCTTCACCGCCATTCTGGTG
  ACCATCGTCATCTTGTATGCGCGCATCTACTGCCTGGTCAAGTCCAGCAGCCGCAGGGTGGCCAACCACA
  ACTCTGAGAGATCCATGGCCCTTCTGCGGACCGTAGTGATTGTGGTGAGTGTGTTCATTGCCTGTTGGTC
  CCCACTTTTTATCCTCTTCCTCATCGACGTGGCCTGCAGGGCAAAGGAGTGCTCCATCCTCTTCAAGAGT
  CAGTGGTTCATCATGCTGGCTGTCCTCAACTCGGCCATGAACCCTGTCATCTACACGCTGGCCAGCAAAG
  AGATGAGGCGCGCCTTCTTCCGGTTGGTGTGCGGCTGTCTAGTCAAGGGCAAGGGGACCCAGGCCTCACC
  CATGCAGCCTGCCCTCGACCCAAGCAGAAGTAAGTCAAGCTCTAGTAACAACAGCAGCCACTCTCCGAAG
  GTCAAGGAAGACCTGCCCCGCGTGGCTACTTCTTCCTGCATCATTGACAAAAACAGGTCGTTTCAGAACG
  GGGTCCTCTGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010101
• Insert Size: 1137 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068176, AAH68176
• RefSeq Size: 4484 bp
• RefSeq ORF: 1137 bp
• Locus ID: 13610
• UniProt ID: Q9Z0U9
• Cytogenetics: 13 A5
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for the lysophospholipid sphingosine 1-phosphate. The gene product functions in endothelial cells and is involved in vascular and heart development. The gene product mediates HDL and HDL-associated lysophospholipid-induced vasorelaxation, and it coordinates with other lysophospholipid receptors in the process of angiogenesis. [provided by RefSeq, Jan 2010]