Bnip3l (NM_009761) Mouse Untagged Clone

CAT#: MC207240

Bnip3l (untagged) - Mouse BCL2/adenovirus E1B interacting protein 3-like (Bnip3l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bnip3l
• Synonyms: C86132; D14Ertd719e; Nip3L; Nix
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207240 representing NM_009761
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCACTTAGTCGAGCCGCCGCCGCCCTTGCACAACAACAACAACAACTGCGAGGAAGGGGAGCAGC
  CCCTGCCGCCGCCCGCTGGCCTCAACAGTTCCTGGGTGGAGCTACCCATGAACAGCAGCAATGGCAATGA
  GAATGGAAATGGGAAGAATGGGGGGCTGGAGCACGTTCCTTCCTCGTCTTCCATCCACAATGGAGACATG
  GAGAAGATCCTCCTGGATGCACAGCATGAGTCGGGACAGAGCAGCTCAAGAGGCAGTTCGCACTGTGACA
  GCCCTTCACCACAAGAAGATGGGCAGATCATGTTTGATGTTGAGATGCATACCAGCAGGGACCACAGCTC
  TCAGTCAGAAGAAGAAGTTGTAGAAGGAGAAAAGGAAGTTGAGGCTTTGAAGAAAAGTGCAGACTGGGTA
  TCAGACTGGTCCAGTAGACCCGAAAACATCCCACCCAAAGAGTTCCATTTCAGACACCCTAAACGTGCTG
  CGTCTCTAAGCATGAGGAAGAGTGGAGCCATGAAGAAAGGGGGCATTTTCTCTGCAGAGTTCCTAAAAGT
  TTTCATCCCATCTCTCTTCCTTTCTCATGTGCTGGCCTTGGGGCTCGGCATCTATATTGGAAAACGACTG
  AGCACACCTTCTGCCAGCACCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009761
• Insert Size: 657 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009761.3, NP_033891.1
• RefSeq Size: 3237 bp
• RefSeq ORF: 657 bp
• Locus ID: 12177
• UniProt ID: Q9Z2F7
• Cytogenetics: 14 34.6 cM
• Gene Summary: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). May function as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function]