1810054D07Rik (BC020085) Mouse Untagged Clone

CAT#: MC206935

1810054D07Rik (untagged) - Mouse RIKEN cDNA 1810054D07 gene (cDNA clone MGC:28310 IMAGE:4013480), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 1810054D07Rik
• Synonyms: RP23-17O21.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC206935 representing BC020085.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGAACTTGAAATATAATGTAAAGGGCACATCAGACTTTATCATCATGGAAGATAGACTTCACACT
  GAATGGTCCTCTCTGACCTGGACAGCCTTTCACATGGGGAACAATTTAAATCCATCCCAATCCATTTCT
  AAAACCTCATTGCTGCAGGAAGAAGCAGCAAGAAGAGCTCTTCTGTGTATTGAGGTGGCTGAGCAGGTT
  CTTAGCCTTAACCATGCAGAAGAAGGATTCGTGGATGCAGCCCCATCCCCTTCTAATTGCACTGTGTAC
  AGTGACAGAGTCAGCACAGTATTGCTGCGACAGGACTTTGTGTGCAAAGATCAAAGCAGCTGCCTCACT
  GTGTTTAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: BC020085
• Insert Size: 357 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020085
• RefSeq Size: 641 bp
• RefSeq ORF: 356 bp
• Locus ID: 69863
• Cytogenetics: 4 C3
• MW: 13.2 kDa
• Gene Summary: Regulates high density lipoprotein (HDL) cholesterol metabolism by promoting the ubiquitination and degradation of the oxysterols receptors LXR (NR1H2 and NR1H3).[UniProtKB/Swiss-Prot Function]