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Ube2e3 (BC011477) Mouse Untagged Clone

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SKU
MC206812
Ube2e3 (untagged) - Mouse ubiquitin-conjugating enzyme E2E 3, UBC4/5 homolog (yeast) (cDNA clone MGC:18411 IMAGE:4195671), (10ug)
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  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ube2e3
Synonyms Ubce4; ubcM2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC011477
CTTGCTGACTGCGCGACCGGGAGGAGCCGAGCCGAGCCGAGCCGAGCGCAGCGGCGGGAGGCTGAGCGCG CGGGGGGCTCCCGCGTCCCTGCGCCTTCGCCTTCGGCCGGCAGAGCGCCCACCCCACCGTGGCTCCCGGA GCGCGGGGCGGCGCCACCCTCCGGCCGCAGCGCGGCACTGCACGGCCGCCTGCGACTTTCGATGTTCCAC ACTCCCGGGCCGGAGCCTCGGCGGCGGCTTCTTTTCCCGCCCCCCCGGTGCCCCCCCCAGGCTGCCTCCA TTTCCTCAAGGAAGGTTCCTCGGCGCTCTCCCTCCCCCACAGCGTAGCGGCGCACGAGCGGGCCGGGCGG GCGGCCGAGAAGTTTTCCCAGAGATAAGTTCACCAAGATGTCCAGTGACAGGCAAAGGTCGGATGATGAG AGCCCTAGCACCAGCAGTGGCAGTTCAGATGCAGATCAGCGAGACCCTGCCGCTCCAGAGCCCGAGGAAC AGGAAGAAAGAAAACCTTCTGCCACCCAGCAGAAGAAAAACACCAAACTCTCTAGCAAAACGACTGCTAA GTTATCCACTAGTGCTAAAAGAATTCAGAAGGAGCTAGCAGAGATAACCCTTGATCCTCCTCCTAACTGC AGTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGGTCAACTATACTTGGTCCACCAGGTTCTGTAT ATGAAGGTGGTGTTTTTTTTCTGGATATCACATTTTCTTCAGATTATCCATTTAAGCCACCAAAGGTTAC TTTCCGTACCAGAATCTATCACTGCAACATCAACAGTCAGGGAGTCATCTGCCTGGATATTCTGAAAGAC AACTGGAGTCCTGCTTTGACTATTTCAAAGGTTTTGCTCTCTATTTGTTCCCTTTTGACAGATTGCAACC CTGCGGATCCTCTGGTCGGAAGCATAGCCACTCAGTATTTGACCAACAGAGCAGAACATGACAGGATAGC CAGACAGTGGACCAAGAGATACGCAACATAATTCACATCATTTGTATGCAGTGTGAAGGAGCAGAAGGCC TCTTCCCACTGTGCTGCGGATCTTTATAGCCTTTACAATACGGACTTCTGTGTATATGTTATACTGATTC TACTCTTGTCTTTATCCTTTGGAGACTGGGAGACTCCCCCAAAAGGTAAAAGCTACCAAGAGTAGAACTT TGTAGCTGTAGATTAGTTATGTTTAAAATGCCTACTTGCAAGTCTTGCTTCTTTGGGATATCAAAATGTA TTTTGTGATGTACTAAGGATACTGGTCCTGAAGTCTACCAAATATTATAGTGCATTTTAGCCTAATTCAT TATCTGTATGAAGTTATAAAAGTAGCTGTAGATGACTAGGAATTATGTCATTTGTATTAAGCCCAGATCT ATTTCTGAGTATGTGGTTCATGCTGTTGTGAAATGTTTTACCTTTGTCAGTTTGTAATGAGAGGATTTCC TTCTACCCTTTGTAGCTCAGAGAGCACTGATGTATCATCTCAAACACAATAAACATGCTCCTGAAGGCAA AAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN BC011477
Insert Size 624 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC011477, AAH11477
RefSeq Size 1562 bp
RefSeq ORF 624 bp
Locus ID 22193
Cytogenetics 2 C3
Summary Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination (By similarity). Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.UniProtKB/Swiss-Prot Function
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