Ifit2 (BC050835) Mouse Untagged Clone

CAT#: MC206773

Ifit2 (untagged) - Mouse interferon-induced protein with tetratricopeptide repeats 2 (cDNA clone MGC:59353 IMAGE:6513417), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ifit2
• Synonyms: AV302338; Ifi54; P54
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050835
  CCGAAAAGCAAGTTCTGGCCTTCTGCAGTTAATGCTTTTTTACCCATCAGCAAGATGCACCAAGATGAGA CTTAGAGGTGCTGCACAGAAGCAGGAAAAGGAACCAGCTGCTCCTATATATAGGTCTGGAGCACTTCATT AGAAGGCAGAGGAAGAGGTTGCCTGGAGAGTGGCTGCAGCCCTGCTTTGAGCGCTTTGACACAGCAGACA GTTACACAGCAGTCATGAGTACAACGAGTAAGGAGTCACTGGAGAGCAATCTGCGACAGCTAAAATGCCA TTTCACCTGGAACCTGATAGCAGAAGATGAGTCCTTGGATGAGTTTGAGGACAGGGTGTTTAACAAGGAT GAGTTTCAGAACAGTGAGTTTAAAGCCACCATGTGCAACATACTGGCCTATGTAAAGCACTGCAGAGGTC TAAATGAGGCAGCACTGCAATGCTTAGGGGAAGCTGAGGGCTTCATCCAGCAACAGCATCCTGACCAAGT AGAAATCAGAAGTCTGGTCACCTGGGGAAACTATGCTTGGGTCTACTATCACATGGGCCAGTTCTCAAAG GCTCAGGCTTATCTGGATAAAGTGAAACAGGTCTGCAAGAAGTTTTCCAGCCCCTACAGGATTGAGAATC CTGCTCTTGACTGTGAGGAGGGGTGGGCCCGATTAAAGTGTACCAAGAACCAAAACGAGAGAGTGAAGGT GTGTTTCCAGAAGGCTCTGGAAAAGGACCCGAAGAACCCAGAATTCACCTCTGGATGGGCCATTGCGAAC TACCGTCTGGATGACTGGCCAGCAAGGAATTACTGCATTGACTCTCTGGAGCAAGCCATTCAACTGTCTC CTGACAACACTTATGTTAAAGTCCTCTTGGCACTGAAGCTTGACGCGGTACATAAAAACCAAGCAATGGC GCTGGTTGAAGAAGCCTTAAAGAAAGACCCAAGTGCAATAGATACACTCCTCAGGGCAGCCAGGTTTTAT TGCAAGGTATATGACACAGACAGAGCTATACAGTTGCTTAGAAAGGCTTTGGAAAAGCTGCCAAACAACG CCTATGTGCATTACTATATGGGGTGCTGTTATAGGTCAAAAGTCCATCATATGTTGAACAGAAGGGAAAT GGTATTTAGTGGGGATAGGAAGAAGTTAGAGGAACTAATACAACTGGCAGTGAATCACTTACGGAAAGCA GAGGAAATCAAGGAGATGCTTGAATATTCCTGTAGCTTCCTTGCTGATCTTTATATCATAGCAAAAAAGT ATGACGAAGCCGATTACTACTTCCAGAAAGAATTGAGCAAGGACCTCCCTCCTGGCCCTAAACAGTTACT CCACCTTCGGTATGGCAACTTTCAGTTTTTTCAAATGAAGCGTCAAGACAAGGCTATCTACCATTATATG GAGGGTGTGAAAATAAAGAAGAAAACAATCCCCCAAAAAAAGATGAGAGAGAAACTTCAAAGAATTGCCC TAAGGCGGCTTCATGAAGATGAGTCTGATTCTGAGGCCTTGCATATCTTGGCATTTCTTCAGGAGAATGG AGGAGGTCAGCAAGCTGATAAAGACTCTGAGAGGGGAGTGGACTCTGCAAACCAGGTACCTTCAGCATCT TTGGATGAAGCAGGTGCTGAATACTAGTGATGTGGTACCCGGCCTACTGCTGGAAGGAAAAAGAAAGCCC TCACCAAGCTGCCATTCAAAATACTTAACCACTTCCTGGATCAAGAATGGGCTATCATACTGACTATAGT ATTTGTCAGGAGTTTGGGGAACTCTGCTATCAGATTCTAGCACCTGAGTTTCTGGATCGTGGGATGGTCT GGGAGAGGGACCAGATGAGGTGATAGATACAGGATCTTGGCAAACAGCCTAGAGCTCTTTCAATTTTTTT TGGAAAAATAGTTTCCACAGTAGTAAATTCTTTAATCACACCCACCCCTCAGTGGAGGCTTGTCTTGGGC TTGTTTCAGTATTTGTATAAAGCAGCCAAATTCCTCACATGGGTGAATGTTCCATCCTTGAATTATTTTA GATATTGCACAGCATTTTCTTCATAGATAATAGTGTCCATCAAAAAGCTGAGGAGTCAGAGACTGTGGCT CAGGACTGTAATCCTAGCACTTGGAATCTGAGACAGGAGGATTACAGTGAACTTGAGGTCAGCCTGAGCA TGACCTGGAAGGGATGGTGGGAGGTGAGAATTATATGTATAAATATTAATGATTATGTACATTACCTTGT TAGTTTGGGTGAGTATATGTCTATATAACCACTATCACATATATCTAAAAATACAGAAGGGAGAGGTTTT GCTTTATTGAATAACTTTAAGACTTACCAAATAATGTTCAATATTAATTGAGCTCAAAATATAAATTGAA TAGTGAGTCCCAGGTCTGGAAGGGATGGTGGGAGGTGAGAATTAAATGTGTAAATATTAATGATTATGTA CATTACCTTGTTAGTTTGGGTGAGTATATGTCTATATAACCACTACTATCACATATATATAAAAACACAG AAGGGAGAGGTTTTGCTTTATTGAATAACTTTAAGACTTACCAAATAATGTTCAATATTAATTGAGCTCA AAATAGAAATTGAATAGTGAGTCCCAGGTCTAAAAACAAACAACAAAAGTTGTGGGACCAAGTCATTCCT CCAATTACCAGCCAGTTCTCAATCTATGGTGTCCCACACACATGAGTTCAACTGTTCCATTAGCTCAGGT GGCAGCAGGATTCGAAAAACTGAGGACAGGGTGAGTCAGAAGGGTCACATCTGTGCAAAGCAGTAGACTC AGGCAGCCACACCAGACTTCCAGGAGTCGCATGGGAAGATGTCTCCCTACTCTGCCCTCCTAGAACTATA TAGTTGTAGGAAAAGAAGGGAGATTCTGTCTACCACCATGACAATTCATATAAACATTTATTTCAAGAGT ATGGAAACACCAGTGGGGATGAAGGGATTGCTGATTTATACGCGATGATTATAAACTCATGAGGAAAAAG TTTATAGTCACTGTTAGGAAGATTTCTCTGTCAAATAAATAATTTATTCAGAAATGCTATAAAGAAATGC CAGGAAGACAGCTGCCTAGAAGGATAACTTGGATTGATGCTTGGGTCAAGGGACTTTGAAAATGTCATGG GTACTGGAGTTGAGTTGTTTTATTCCATGTGAAATGTGTCACACCAAGGGATATAAATAATTTTTATAAC TCTGAAAAAAAATACAGAAACACCACAAACTTGATAATTGCCATTTCCTAGGTAGATCTGGAAGGGCCTA AATGTTAAGGTTGGTGTGAATTGACAGAGTCAAGTGGCCCAGTATGAGACGGTCAGCCACACTCTGGGAT AAAAAAATGTTTCTCTACTGGGTTCAGCTTGTCTCTATGTATATATGAACATTTTATTCAGCAATTAAAA CATCCATGATTCATAGATTGTTAAAACTAAACATTCTAACAGCTGTATCAGTGCTTCATAATATTTTAAA AATGAAAATATTCTCTATGCATTAAGTCCATCGATATTCACCATTTGAATTTCTGGAAAAATAATAAACT TTGTTTATAAGTAACACATAATGTAACATTAAGCTATGCTCTGGTTCCCCTGAACTTTACTGTACCTTCG TATTTTTAGACTCAAGTACTGATAATAAAGAGTGCATTAACTGTTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC050835
• Insert Size: 1413 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050835, AAH50835
• RefSeq Size: 3720 bp
• RefSeq ORF: 1413 bp
• Locus ID: 15958
• Cytogenetics: 19 C1
• Gene Summary: IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.[UniProtKB/Swiss-Prot Function]