C430003P19Rik (BC030838) Mouse Untagged Clone

CAT#: MC206746

C430003P19Rik (untagged) - Mouse RIKEN cDNA C430003P19 gene (cDNA clone MGC:31025 IMAGE:4238232), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C430003P19Rik
• Synonyms: AA589499; Abro1; AI853413; C430003P19Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030838
  GATTATGCGAGCAAAGTGAATGAAGAAAGTTTGGACAGAATTCTGAAAGATCGGAGGAAGAAAGTTATCG GATGGTACAGGTTCCGGCGAAACACCCAGCAGCAGATGTCCTACAGGGAACAGGTCATCCACAAGCAGCT CACCCGCATCCTCGGCGTGCCAGACCTGGTCTTCCTTCTCTTCAGCTTCATCTCTACCGCCAACAATTCT ACTCATGCTTTAGAATATGTTCTCTTTAGACCAAACCGCAGGTATAATCAAAGGATATCACTTGCTATTC CCAATCTAGGCAATACTAGCCAGCAAGAGTACAAAGTGTCTTCAGTGCCAAACACGTCTCAGAGTTATGC CAAGGTCATTAAAGAGCACGGTACTGACTTTTTTGACAAGGATGGAGTCATGAAGGACATCAGGGCAATT TATCAGGTTTATAATGCACTTCAGGAGAAAGTACAGGCAGTATGTGCAGATGTAGAAAAGAGTGAGCGAG TTGTTGAATCTTGTCAGGCAGAAGTGAACAAATTGAGAAGACAAATCACTCAGAAGAAAAATGAAAAGGA ACAAGAAAGAAGATTGCAGCAGGCCCTGTTAAGCAGACAGATGCCATCCGAAAGTCTGGAGCCAGCGTTC AGCCCTCGGATGTCCTATTCTGGGTTTTCAGCTGAAGGGAGAAGTACTCTGGCAGAAACGGAGCCCTCTG ATCCTCCGCCCCCTTACTCTGATTTCCACCCAAACAATCAAGAAAGTACTCTGAGCCATTCTCGCATGGA AAGGAGTGTCTTCATGCCCCGTCCTCAGGCTGTGGGCTCTTCCAGCTATGCTTCCACCAGTGGCGGACTG AAGTTCACTGGAAGTGGAGCAGATCTTCTTCCTTCCCAGAGTGCAGCTGGAGACAGTGGGGAGGAGTCAG ATGACAGTGATTATGAAAATTTGATTGATCCTGCAGAGTCCCCTCATAGCGAATACTCACATTCAAAGAA TTCACGGCCCTCGACACATCCTGACGAGGACCCCAGGAACACTCAGACCTCCCAGATTTAAGTAAACAAA TGACACTCTCCACTTGGCACTACTTTTCTTAATGGCTTATTGAGTGTTGTTGAGGACTTAATCTGTGGGG AGACCTGCTTTCTCAGATGCCGCGGCTCCTGAGTGGAGCATCCTGTGCACAGAACTCCCAGAATCCTCTG TCCGCTGGTCCTCACCTCTGGGTACAGTGCACAGGTTCACAACAAAATCATTACGATAATCAAATTGTCC TAATTCTGGTGCGATTCATGGATGTACTAGTGAATTTAGGCAGAGTGACCCCTCAGTGTAGTCTGTTCTT AAGATAAATCAGATCAGAGACTGAGCACAATTAGTCTATAAATAGTCTGTACGTCAGAAACTTACCTCTG CACTATCATGCCTTGAAACTTGCTTTTTCAAAGGGAAACGAATTTAGCAGCAGCCTTCAAAGAACTTTCT ATGATGAGCCAAATTCATCTTTGCCAGAAAAGAAATTTTGTTAATTCCAAGAAGCCTAATTGGAACAAGT CAGACATTCCTTCTCTCATCTGCATGGTTTTGTAAGATGAGAAGAGAGGGCTTTGTTTCAACTTTTTTCT ACTAGCCTGATACGTATTGTCACTTAAAATGGTTGCCTTTTTAAAATGTAGGAATACTAATCCCCAGCGA GTACTCATCTGGATCAGGCCCTTGTCTTTTGGTGCACGCCAGCGATGCTGCGCTGCATGCAGTCATGGAG GGAGGCCACACACCAGCAGATGGCAGAAGGGAGAAGCCAGGCCTGAGTCTGTAGTGAACAGATCCCAGAC ACAGTGGATTTAACTAAGTTCCTATGGAAAGGATGTAATGTACTTAGGTGTGGCGGATCCTGGGCTAGAG TTGTGGAGGAAAGACGTTTTTGAGAAAATATCCAGGAATCTTGGTGACTACAGAGCTACTTCAAGTATGA ATAACCATACTTCTTTTTTCCCCTAAAGTTGCCATTTAGGGGATAATTGCAAATGTGCGGAGACCATTGG GATGCACTTACGCTTTTATTTAATTACTGCTTGTTTTCTAGTTTTGCCCAGAATGTGTGAAACCACTAAA AACCATTTTTGAGTATGTTGGGCTGCTGGATTGGAAGTGGCAAGACCTACCATATATGGCAATCAATCAG CCCATGATGCTTGGCGCTGAACTGCCTTCCTTGGACTAGTTAAAACGCGTAAGGATGAGGATACCGTTAA AAGGTTTCAAAGGAGTCATCTCGTTTGATAGTGATAGTTACTCAGGAGCTCCAGTGCGCAGTCTCAGACA GTGGATACAGTCTTAGAGACGAGATGCTGACCGTCCTGCTTTGATTGTGCCCCAGGGTCTGCATTGTAAC TGCTATCCTGGTTTTCTGGGCTCACACCACTTCCGTTTGATTTGAATGTCGAGGGTCACTTTATCTGCAG TGCGTTCCAGCTTTTATCAGCTACCTGCTAGCAGCTTCAGCACGGTATGAAGTCCAGTCTGTTAAAGTGC CATCGCCATCTCTTCTGTTCAGATTTTGACATTCAGGAAAATATTCTTATTTTTATGCCATACTGAAATA TACAGTGTATATCTGACAAAGCAGTTAAATGTGACAATAAAAACTTATTTAATCATGAAAAAAAAAAAAA AA
• Restriction Sites: RsrII-NotI
• ACCN: BC030838
• Insert Size: 1041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030838, AAH30838
• RefSeq Size: 2662 bp
• RefSeq ORF: 1041 bp
• Locus ID: 109359
• Cytogenetics: 7 F3
• Gene Summary: Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked polyubiquitin, leaving the last ubiquitin chain attached to its substrates. May act as a central scaffold protein that assembles the various components of the BRISC complex and retains them in the cytoplasm (By similarity). Plays a role in regulating the onset of apoptosis via its role in modulating 'Lys-63'-linked ubiquitination of target proteins (PubMed:21195082). Required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activities by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). Required for normal induction of p53/TP53 in response to DNA damage. Independent of the BRISC complex, promotes interaction between USP7 and p53/TP53, and thereby promotes deubiquitination of p53/TP53, preventing its degradation and resulting in increased p53/TP53-mediated transcription regulation and p53/TP53-dependent apoptosis in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function]