Chfr (BC049792) Mouse Untagged Clone

CAT#: MC206709

Chfr (untagged) - Mouse checkpoint with forkhead and ring finger domains (cDNA clone MGC:59419 IMAGE:6510480), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chfr
• Synonyms: RNF116, C230082M18
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049792
  CTGTTGACAGCGGTGGCGGCGACTGTAAGGCCGGTTCCGGGGTCCGCGCAGGGCGTGGGATGTGAGTCCT ATGGAGCTACACGGGGAAGAGCAGCCGCCGCCGCCGCAGGAACCCTGGGGGAGGCTTCTTCGTCTAGGCG CAGAGGAGGACGAACCCCAGATCCTTCTTTGGAAACGCGAGTGGACCATCGGAAGGAGGAGAGGCTGTGA CCTCTCTTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAAACTTACAGTGGATGAAATATCTGGT GAGGTGACACTGGAAGACACCAGCACCAATGGAACAGTGATCAATAAGCTGCAAGTTGTTAAGAAGCAGA CTTACCCTTTACAGAGCGGGGATATCATCTATTTGGTGTACAGGAAGAATGAGCCAGAACACAATGTGGC ATACCTCTATGAATCTTTAAGTGGCAAACAGAGCTTAACTCAAGACTCCCTTGAAGCCAATAAGGAAAAT ATGTTCCATGTGACCAAAGATTGCTCAGGTCCAGGGCAGGGTGATGATCCCCAGGTTCCACTATTGTCAC CCATGGCTCAGACATGCTTAGAGGAACCACAGCCATCAACATCGACATCAGACCTCCTCCCCACGGCCTC TACCTCTTCTACGGAGCCAGAGCTGACCTCTGCAGGGCAAAAGCATTCTTCTAGCTCTGGACCTGGGAAC ACAAGCATCTCCCCAAAAGGACGCAGTTCACTTGTTGCAAATGGCGAACTCTCTAGCCTTTCTCCAGTTT TCCAAGACAAAGAAGCATCCTTTTCTTTGCTGGAAAGTAAAGACCATGAGGAATTGGAGCCTGCCAAAAA AAAGATGAAAGGAGATGGGGAACTTGACACGAACCTCCAGTTATTAGTTTCAGGCCAGCGTGGAAATGCC CAAACCTCAAGTGAAGATGTCAAAGATGCCTCTGTGAAGCCAGACAAGATGGAGGAGACACTAACCTGTA TCATCTGCCAGGACCTTCTGCACGATTGTGTGAGTTTGCAGCCTTGTATGCACACATTTTGTGCGGCTTG CTACTCTGGTTGGATGGAGCGTTCATCTCTGTGCCCTACCTGCCGATGTCCAGTGGAGCGGATTTGCAAA AACCACATCCTGAACAACCTAGTGGAAGCATACCTTATCCAGCACCCAGATAAAAGTCGCAGTGAAGAAG ATGTGAGAAGTATGGATGCAAGGAATAAAATCACTCAAGATATGCTGCAACCCAAAGTCAGGAGGTCTTT CTCTGATGAAGAGGGGAGTTCAGAGGACCTGCTAGAGCTGTCTGATGTCGACAGTGAATCCTCAGATATC AGTCAGCCATACATTGTCTGCAGACAGTGTCCTGAATACAGAAGGCAAGCGGTGCAGTCTCTTCCTTGCC CAGTCCCAGAGAGTGAGCTGGGAGCTACACTGGCCCTTGGTGGGGAGGCACCTTCAACATCTGCCAGCTT GCCAACAGCAGCCCCAGATTACATGTGCCCTCTTCAAGGAAGCCATGCCATATGCACCTGCTGCTTCCAG CCTATGCCTGACCGGAGAGCTGAACGGGAGCAGGATCCCCGCGTCGCCCCTCAGCAGTGTGCGGTGTGCC TGCAGCCCTTCTGCCACCTGTACTGGGGCTGCACGAGGACTGGCTGCTTTGGCTGCTTGGCCCCATTCTG TGAGCTCAACCTGGGGGACAAGTGCTTGGATGGAGTGCTGAACAATAACAATTATGAATCGGACATCCTG AAGAATTACCTGGCAACCAGGGGTCTGACATGGAAAAGTGTGTTGACAGAGAGTCTCCTGGCTCTGCAGC GAGGTGTATTTATGCTGTCTGATTACAGAATCACTGGAAATACTGTGCTGTGTTACTGCTGTGGTCTGCG TAGCTTCCGAGAGCTGACCTACCAGTATCGTCAGAACATTCCTGCTTCTGAGTTGCCAGTGACTGTAACA TCCCGTCCTGACTGCTACTGGGGCCGCAACTGTCGCACTCAGGTGAAGGCTCACCATGCAATGAAATTCA ATCACATCTGTGAGCAAACAAGGTTCAAGAACTGAATATTGGAAGAGACACGTGAAACAGTTACAGAGCA CAGGAGGCAATGTTTTTTAAAAGGCCCCATACAAACACTTGAAGGGCATTTTTACAGTTTTCCCTACGCA GCACTGGGGGTTCTGGTCAGGCACTGGGGTGTGGATTTTCCCTTTGTGGAGCATCATCGTCAGTGACACA CACACCACGTGCCCCAGTAACCTGGGAGGCCTCCTGGCCACACCAGCCTTCTAGTCGGTGTAAGAGGATC AGTAGACTGAGTTCTCCCTCCCTCCCACTGGACCGCTCTCCCTCCTGTGTGCTGAGGCAGTGACAGTGCA GGTTTGTACTTTTGTATCTTTTCTACCTTGTACCTTGACTTTGGTTAAAATGGGAAGCTCATAGGAAAAG TTGTCAGCTGTGTCAGACGCATTCTCCGGAAGGAAAGGAAGCATACGTTTACAGCCTCCAGGGGTACATA GGATAGTCTCCTGTCTGGAAAGACACAGCCTTTTGTATATTTTTTATTTTAATAAGTTTGGTGCTTATCT TCTAATAAGATTTAAATATTAAAAACTGTAGCACAAATACTATAATTTATAATTTACAAATTGACTAAAA TTAGGAATAGCCTCATATTTGGAAGAATGAGCATATGTTTCTGTTTATTTAAAAAAAATTATCAACTGTC CGAAACTGCTAATCCTGGGAAGGAAGTCTTGAGTGTCCAGGTCCTCGACCCAGGGCTGGCTGAGCCCTTA GCCCTCCAGTCCCTGAGAGCAACTCCCTGCTGAAACTCCAGATGGCTCCAGACTGACAGGGCCTTTGCTT TCCAAGCCCTCATCTGGTACATTAGGTTTGCCATGTCATCTGGAATAATACTTGAAATTTTGTTTTTAGG TTTTTTAAAACTTTTTTATCTTTTATTGAAATCACCTGTTATATTTGTTTGAAAACTGGTAACTTTTTGC TTCTCAGGAAAAAAATTTTCAACTGTTGTCTTGCTCTTGATACAGACTGAGAGGCAGTACTCTGTGTTTG TGGGAGATGGTTCTTTTTTTGATTCTAATAAATGAGCTGTGTTTGTTTCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC049792
• Insert Size: 1995 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049792, AAH49792
• RefSeq Size: 3194 bp
• RefSeq ORF: 1995 bp
• Locus ID: 231600
• Cytogenetics: 5 F
• Gene Summary: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity).[UniProtKB/Swiss-Prot Function]