Mdm2 (BC050902) Mouse Untagged Clone

CAT#: MC206693

Mdm2 (untagged) - Mouse transformed mouse 3T3 cell double minute 2 (cDNA clone MGC:63226 IMAGE:6415937), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdm2
• Synonyms: 1700007J15Rik; AA415488; Mdm-2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050902
  GGCCGGTGAGGAGCCGCCGCCTTCTCGTCGCTCGAGCTCTGGAGCGACCATGGTCGCTCAGGCCCCGGCC GCGGGGCCTCCGCGCTCCCCGTGAAGGGTCGGAAGATGCGCGGGAAGTAGCAGCCGTCTGCTGGGCGAGC GGGAGACCGACCGGACACCCCTGGGGGACCCTCTCGGATCACCGCGCTTCTCCTGCGGGCCTCCAGGCCA ATGTGCAATACCAACATGTCTGTGTCTACCGAGGGTGCTGCAAGCACCTCACAGATTCCAGCTTCGGAAC AAGAGACTCTGGTTAGACCAAAACCATTGCTTTTGAAGTTGTTAAAGTCCGTTGGAGCGCAAAACGACAC TTACACTATGAAAGAGATTATATTTTATATTGGCCAGTATATTATGACTAAGAGGTTATATGACGAGAAG CAGCAGCACATTGTGTATTGTTCAAATGATCTCCTAGGAGATGTGTTTGGAGTCCCGAGTTTCTCTGTGA AGGAGCACAGGAAAATATATGCAATGATCTACAGAAATTTAGTGGCTGTAAGTCAGCAAGACTCTGGCAC ATCGCTGAGTGAGAGCAGACGTCAGCCTGAAGGTGGGAGTGATCTGAAGGATCCTTTGCAAGCGCCACCA GAAGAGAAACCTTCATCTTCTGATTTAATTTCTAGACTGTCTACCTCATCTAGAAGGAGATCCATTAGTG AGACAGAAGAGAACACAGATGAGCTACCTGGGGAGCGGCACCGGAAGCGCCGCAGGTCCCTGTCCTTTGA TCCGAGCCTGGGTCTGTGTGAGCTGAGGGAGATGTGCAGCGGCGGCAGCAGCAGCAGTAGCAGCAGCAGC AGCGAGTCCACAGAGACGCCCTCGCATCAGGATCTTGACGATGGCGTAAGTGAGCATTCTGGTGATTGCC TGGATCAGGATTCAGTTTCTGATCAGTTTAGCGTGGAATTTGAAGTTGAGTCTCTGGACTCGGAAGATTA CAGCCTGAGTGACGAAGGGCACGAGCTCTCAGATGAGGATGATGAGGTCTATCGGGTCACAGTCTATCAG ACAGGAGAAAGCGATACAGACTCTTTTGAAGGAGATCCTGAGATTTCCTTAGCTGACTATTGGAAGTGTA CCTCATGCAATGAAATGAATCCTCCCCTTCCATCACACTGCAAAAGATGCTGGACCCTTCGTGAGAACTG GCTTCCAGACGATAAGGGGAAAGATAAAGTGGAAATCTCTGAAAAAGCCAAACTGGAAAACTCAGCTCAG GCAGAAGAAGGCTTGGATGTGCCTGATGGCAAAAAGCTGACAGAGAATGATGCTAAAGAGCCATGTGCTG AGGAGGACAGCGAGGAGAAGGCCGAACAGACGCCCCTGTCCCAGGAGAGTGACGACTATTCCCAACCATC GACTTCCAGCAGCATTGTTTATAGCAGCCAAGAAAGCGTGAAAGAGTTGAAGGAGGAAACGCAGGACAAA GACGAGAGTGTGGAATCTAGCTTCTCCCTGAATGCCATCGAACCATGTGTGATCTGCCAGGGGCGGCCTA AAAATGGCTGCATTGTTCACGGCAAGACTGGACACCTCATGTCATGTTTCACGTGTGCAAAGAAGCTAAA AAAAAGAAACAAGCCCTGCCCAGTGTGCAGACAGCCAATCCAAATGATTGTGCTAACTTACTTCAACTAG CTGACCTGCTCACAAAAATAGAATTTTATATTTCTAACTATATGACCCCCAAATTAGACAACATGGGTAT TATTTTTATACATTAAAGCCAGAAAACTGTCTTAGTCCACATAAAATTCACTTATAATTTATCCTGGAGA GTAAATATGGTGAATATTTTCTTCCTTTTTAGGGAAATTTCACTTGTTTATTTTATATTTGTGTTTTAAG TAATTTGCATTGGCTGTTTATATTTTCCTTATATTTTAAATAATCTCCGCTTGGAAGGACTTTGGAAGTG TATGTGAGAAGTCCTTTCCATCTCCTGCAGATGATGGTGGACCTTCCTCATCAAGGGCTACAGAAGTACT TGATTTCTGTTTTTTTTGTTAATAATAAGAACATTTAATTTATTTAGTGTCTTTCATGTAAAGAGTTAAA GACTATGTGAAGGATTGTATATTTAAGTTATTGAAATTCTGAAACTGTAGTAATCTAAAATGTGTGAGTT GTGGGCTGCAGAGAAGACTCAGCCAGTAAAGGCACCTGCTGTGTACACCCACTGACACACATTTGATCCT TGGAACCCCCAGGAAGAGTGAACCAGTTCCACAAAGTTGTTCCCTGATCTTCAAATGGATGCACGCATGC ACGCACACACACGCGCGCGTGCGCACACACACACACACACACACACACACACACACACACACACACACAC ACACAGTTTTAAAGGCATGAATTGCATCTGGTGGTATGTAAGTGAAAACACACGCCTTATTTTCCAGCAT TTTCAGCTTTTTGTCATAGGGGTGTGGCACAAGTGTTGCAGTTTGTCCCAGGTTGAAAAGCCTGAGGCTG GTAGAAGCGCCTTTTTGCCTCAGCTCCGTGGTTCCTGGTGGTTGCCTATGTTTCAGGCCTGTACTTAGGC TAGGTTTAGAAACCAGCCCATTCAGAAAGACTGAATCAGAACATGGATAAAGTGAACTCATTCTAAGATG ACTCGTCTATCCATGTAGATTAATCTCCTGGTTCATAATAGGCCTCTTCCCTTTGATTGAAGGGTCACGT CTAAGTATAGAAAACATAAAACTGTAAGGTAGAGGAAGCGAAGGATAGCTTTGTATTAATGTTGCGTTAA AGCTTCAGAGACAAGAACAAGAACACTCCTCCCACGTGACAGCATTTGAATAGGAGGCGGTGGGTGCGGC AGCCTGGGCAGCTTCAGTCCCGATTTACAATAAAGTACCTTGTGTGTTATTAGTTCTTAAATGTTTATTT AGAAATGGCATTGATGTTATTTATTTGCAAATAAATGGTTTATTGAAGAATTGTGAAAAAAAAAAAAAAA AAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC050902
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050902, AAH50902
• RefSeq Size: 3014 bp
• RefSeq ORF: 1470 bp
• Locus ID: 17246
• Cytogenetics: 10 66.32 cM
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome (PubMed:15195100, PubMed:21804542). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain (By similarity). Also acts as a ubiquitin ligase E3 toward itself, ARRB1 and ARBB2 (PubMed:11588219). Permits the nuclear export of p53/TP53 (By similarity). Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein (By similarity). Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation (By similarity). Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53 (By similarity). Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways (By similarity). Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus (By similarity). Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (By similarity). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (PubMed:25088421). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (PubMed:14642282).[UniProtKB/Swiss-Prot Function]