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Eftud2 (BC052674) Mouse Untagged Clone

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SKU
MC206668
Eftud2 (untagged) - Mouse elongation factor Tu GTP binding domain containing 2 (cDNA clone MGC:60573 IMAGE:30061580), (10ug)
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Eftud2
Synonyms U5-116kD, 116kDa
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC052674
AAGAGGTAATCCCGGGCTCAGGCGGCGGACCGGGGCACTCGGCTGAGCATTCGGCGGCATCATGGATACT GACTTGTATGATGAGTTTGGGAATTATATTGGACCGGAGCTTGATTCTGATGAAGATGATGATGAACTGG GAAGAGAGACAAAAGATCTTGATGAGGATGAGGACGAGGACGAGGATGACGTGGGAGAGCACGAGGATGA CCACCCCGGCATGGAGGTGGTGCTGCATGAGGACAAGAAGTACTACCCCACGGCCGAGGAGGTGTACGGT CCCGAGGTGGAGACCATAGTCCAGGAGGAAGACACCCAGCCCCTCACAGAACCCATTATAAAGCCAGTGA AAACCAAGAAATTCACTCTGATGGAGCAGACATTACCCGTCACGGTGTATGAGATGGATTTCTTAGCAGA TCTGATGGACAACTCTGAGCTCATCAGAAACGTAACCTTGTGTGGCCATCTCCACCACGGCAAGACCTGT TTCGTAGATTGTTTAATAGAACAGACGCATCCGGAAATCAGAAAGCGCTATGACCAAGACCTATGCTATA CTGACATCCTCTTCACAGAACAAGAGAGAGGCGTCGGCATCAAGAGCACTCCGGTGACGGTGGTGCTGCC GGACACCAAAGGGAAGTCTTACCTCTTCAACATCATGGACACGCCAGGTCATGTGAATTTTTCTGATGAG GTCACAGCTGGCTTGCGCATCTCAGATGGAGTGGTCCTTTTCATTGATGCTGCTGAGGGGGTGATGCTGA ACACAGAGCGGCTGATCAAGCACGCAGTGCAGGAAAGGTTAGCAGTCACCGTGTGTATCAACAAAATCGA CCGGCTGATCTTGGAGCTGAAGCTGCCTCCAACAGATGCTTATTACAAGCTGCGACACATTGTGGATGAG GTCAATGGATTAATAAGCATGTATTCCACCGACGAGAACCTGATCCTTTCTCCACTCCTGGGCAACGTCT GCTTCTCCAGTTCCCAGTACAGCATCTGCTTCACACTGGGTTCCTTTGCCAAGATCTACGCTGACACCTT TGGTGACATTAATTACCAGGAATTTGCTAAAAGACTCTGGGGTGACATCTACTTCAATCCTAAGACGAGA AAGTTCACCAAAAAGGCTCCCAGCAGCAGCTCCCAGAGGAGCTTCGTGGAGTTCATCTTGGAGCCACTCT ATAAGATCCTGGCCCAGGTGGTGGGTGATGTGGACACCAGCCTGCCAAGGACCCTGGACGAACTTGGCAT CCACCTCACCAAGGAAGAGCTAAAGCTGAACATCCGCCCCCTGCTCAGGCTGGTCTGCAAAAAGTTCTTT GGCGAGTTCACAGGCTTTGTGGACATGTGTGTGCAGCACATTCCATCTCCAAAGGTGGGCGCCAAACCCA AGATCGAGCATACCTACACTGGCGGCGTGGACTCTGACCTTGGCGAGGCCATGAGCGACTGTGACCCAGA TGGTCCCCTGATGTGCCACACGACGAAGATGTACAGCACAGATGATGGAGTCCAGTTTCATGCCTTCGGC CGGGTGCTGAGCGGCACCATCCACGCTGGGCAGCCTGTGAAGGTGCTGGGGGAGAACTACACCTTGGAAG ACGAGGAAGATTCCCAGATCTGCACCGTGGGCCGCCTCTGGATCTCCGTCGCCAGGTACCACATTGAGGT GAATCGTGTTCCTGCTGGCAACTGGGTCCTGATTGAAGGTGTTGACCAGCCGATCGTGAAGACAGCAACC ATAACTGAACCCCGAGGCAATGAGGAGGCTCAGATTTTCCGGCCCTTGAAATTCAATACCACATCTGTTA TCAAGATTGCCGTGGAGCCGGTTAACCCCTCAGAGCTGCCCAAAATGCTTGATGGCCTGCGCAAGGTCAA CAAGAGCTATCCATCTCTCACCACCAAGGTGGAAGAGTCTGGCGAGCATGTGATCCTGGGCACTGGGGAG CTCTACCTGGACTGTGTGATGCACGACCTGCGGAAGATGTACTCAGAGATCGACATCAAGGTGGCTGACC CAGTTGTGACATTCTGTGAGACAGTGGTAGAAACGTCCTCCCTCAAATGCTTTGCTGAGACACCCAATAA GAAGAACAAGATCACCATGATTGCCGAGCCCCTTGAGAAGGGCCTTGCTGAGGACATAGAGAATGAAGTG GTCCAGATCGCCTGGAACAGAAAGAAGCTGGGAGAGTTTTTCCAAACAAAGTACGACTGGGATCTGCTGG CTGCCCGTTCCATCCGGGCCTTCGGACCTGATGCTACAGGCCCCAACATCCTAGTAGATGATACACTGCC CTCAGAGGTAGACAAGGCCCTTCTCGGCTCAGTGAAGGACAGCATTGTTCAAGGTTTCCAGTGGGGGACC AGGGAAGGACCCCTCTGCGATGAATTGATTCGGAATGTCAAGTTTAAGATACTGGATGCTGTGGTTGCCC AGGAACCCCTCCACCGGGGTGGAGGTCAGATCATTCCCACAGCCAGGAGGGTAGTGTACTCTGCCTTCCT CATGGCCACTCCCCGTCTGATGGAGCCTTACTACTTTGTAGAGGTCCAGGCTCCTGCAGATTGTGTGTCT GCTGTTTACACAGTCCTGGCCAGACGCAGGGGTCATGTGACTCAGGATGCACCCATACCAGGCTCCCCTC TCTATACCATCAAAGCGTTCATCCCGGCCATCGACTCATTCGGCTTTGAGACTGATCTCCGCACTCACAC CCAGGGACAGGCCTTTTCCCTGTCTGTCTTCCACCATTGGCAGATTGTCCCAGGCGATCCTCTGGACAAG AGCATTGTCATTCGCCCGCTGGAGCCACAGCCAGCTCCTCACCTGGCTCGGGAGTTCATGATCAAAACCC GTCGTAGGAAGGGCCTGAGTGAAGATGTGAGCATCAGCAAGTTCTTCGACGATCCTATGTTGCTGGAGCT CGCCAAACAGGATGTTGTGCTCAATTATCCCATGTGAGTGAAGGGGCTGGCAGCTCTAGACTCAAGCCGA GACCTGGTGACTGGGCCCTCGAGCTCTGAGCATCGGGAACTGCTATGGGGCCGTCTGAACGCCCCAGAAA CCTCCATCCCAGTGAGGAAGGGGCATCTGGCCTGGTTTCTTCTGTGGCCTTTGCCTGGCCCCATTCCCAA GGAACAGAGGAGAGCTTGGGCCCAGAGAAGAAGAATGAGGACAAGATGAAAATGTTTAACCCTAAGGGGC CTGTCTTCAGGACTTACATGGAATTTTATTTTTTACAAGTTCCACTTTAGCCACAGTTTATAAATGAACA GGACATTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites EcoRI-NotI |
ACCN BC052674
Insert Size 2916 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052674, AAH52674
RefSeq Size 3345 bp
RefSeq ORF 2916 bp
Locus ID 20624
Cytogenetics 11 E1
Summary Required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome.UniProtKB/Swiss-Prot Function
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