Mov10 (BC053743) Mouse Untagged Clone

CAT#: MC206654

Mov10 (untagged) - Mouse Moloney leukemia virus 10 (cDNA clone MGC:59369 IMAGE:6510959), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mov10
• Synonyms: C77703; Mov-10
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC053743
  GGAGAAGGTGGCTGGCCGGAGCAGACGTCAGAGGTAGGGGGCTTTTCCCCTTTGCACCCTCACCCCAGAC CAGCCAACTCTAAACCGCGGCGGCGACTTTCAGTTTCATTTCCGCGGACCCTCCTGCCTGGGCCGCAGCC GCCGCCGCCGCGATGCCTAGCAAGTTCAGCTGCCGAAAGCTCCGGGAGACCGGCCAGAGGTTCGAGAGTT TTCTGGCCGAACGTGGACTGGACCTGGAGACAGATCGTGAGCGGCTGCGGACGATTTACAACCACGACTT CAAGCCCAGCTATGGGACCCCTGCCCCTGGCTTCTCCTCCATGCTGTATGGAATGAAGATCGCAAATCTG GCCTTCGTCACCAAGACTCGGGTCAGGTTCTTCAAACTAGACCGCTGGGCTGATGTGCAGTTACCAGAAA AGAGGCGAATAAAGCCAGGGTCGAACATCAGCAAACAACACAGATCACTGTTGGCCAGGATCTTTCACGA CAGGGCTGAGTACCTTCATGGGAAGCATGGGGTAGACGTGGAGGTCCAGGGGCCCCATGAAGCCCGAGAC GGGCAACTCCTTATCCACCTGGATTTGAACCGCAAGGAGGTACTAACCCTACGGCTTCGGAACGGCGGAA GCAAACCTGTCACCCTCACTCACCTATTCCCACTGTGCTGGACGCCCCAGTTTGTCTTCTACCATGGAGA ACAGGACCTGCCCTGCCCACTGGGCCCCGGTGAAAGCTATGAACTCCACATCTACTGTAAGACCAGCATT GTGGGTTACTTCCCAGCCACTGTCCTCTGGGAACTCCTGGGACCCGGGGAGTCGGGAGCAGAAGGAGCAG AAACTTTCTACATTGCCCGATTCCTGGCGGCTGTCGCCCACAGTCCCCTGGCTGCCCAGTTGAAACCCAC AACTCCCTTCAAACGCCCCCCTCGGCTCACCAGAAACTCTGTGTTGACCAACCGGATCGAGGAAGGAGAG AGGCCCGACCGAGCCAAGGGCTATGAACTAGAGCTAAGTTTGGCCCTGGGGACCTATTACCCACCCATCC TCCTCCGACAACTGCTCCCTACCCTTCTTCAGGGACCAAGTATCTTCACTGCCCCAAAGGAGGTTGCTGA GATCAAGGCCCAGCTGGAGACAACCCTGAAATCCAGGAACTATGAGGTGAAACTCCGGCTGCTGCTGCAC CTGGAAGAGCTGCAGATGGAGCATGACATCCGGCACTATGACCTGGACTCGGTACCCATGACCTGGGACC CTGTGGACCAGAATCCCAGGCTGCTCACCCTGGAGGTTCCTGGTGTCGCAGAGAGCCGTCCCTCAGTGCT ACGAGGTGACCACCTTTTTGCCCTTTTGTCCTCTGAGACCCAACAGGACGACCCTGTCACCTACAAGGGT TTCGTGCACAAGGTGGAACTGGACCGTGTCAAGCTGAGCTTTTCTACAAGCCTCCTGAGCCGATTTGTGG ATGGGCTGACCTTCAAGGTGAACTTTACCTTCAACCGCCAGCCCCTTCGGGTCCAGCACCGGGCCCTAGA GTTGACGGGGCGCTGGGTGCTATGGCCCATGCTTTTTCCTGTGGCCTCCCGTGGGGTCTCGTTGCTGCCC TCAGATGTGAAGTTCAAGCTGTACGATCGGAGTCTGGAGTCAAACCCTGAGCAACTGCAGGCCATGAAGC ACATTGTGAGGGGTACCACCCGGCCTGCCCCCTACATCATCTTTGGGCCTCCAGGTACCGGCAAGACTGT CACATTAGTGGAGGCCATCAAACAGGTAGTGAAGCATTTGCCCAAAGCCCACATCCTGGCCTGTGCTCCA TCCAACTCAGGGGCTGACCTCCTCTGTCAGCGGCTCCGGGTCCACCTGCCCAGCTCCATCTACCGTCTCC TGGCCCCCAGCAGGGACATCCGAATGGTGCCTGAGGACATTAAGACCTGCTGTAACTGGGATGCTAAGAA GGGAGAATATGTGTATCCTGCTAAGAAGCACCTGCAGCAATATCGGGTCTTAATTACCACCCTCATCACT GCCAGCAGGTTGGTGTCAGCCCAGTTTCCCATCGATCACTTCACACACATCTTCATCGATGAGGCTGGCC ACTGCATGGAGCCTGAGAGTCTGGTGGCCATAGCAGGACTGATGGATGTCAAGGAAACGGGCAATCCCGG AGGGCAGCTGGTACTGGCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGCTCGCCACTAGCCCTGAAG CATGGACTTGGCTACTCTCTGCTAGAGCGCCTGCTCGCCTACAACTCCTTGTATAAGAAGGGCCCCAATG GCTATGACCCCCAGTTCATCACCAAACTGCTCCGCAACTACAGGTCTCACCCCACCATCCTGGACATCCC TAACCAGCTGTACTACGACGGAGAGCTGCAGGCCTGCGCAGATGTGGTGGATCGAGAACGGTTCTGCCGC TGGGAGGGGCTGCCTCAGCAGGGCTTCCCCATCATTTTCCATGGTGTAATGGGCAAAGATGAGAGAGAAG GCAATAGCCCATCCTTCTTCAACCCTGAAGAGGCTGCTACAGTGACGTCATACCTGAAACAACTCCTGGC CCCTTCCTCCAAGAAGGGTAAAGCCCGCCTGAGCCCCCGAAATGTGGGCGTCATCTCCCCGTACCGGAAG CAGGTAGAAAAAATCCGTTACTGCATCACAAAACTTGACCGAGAACTTCGCGGACTGGATGACATCAAAG ATTTGAAGGTGGGCTCTGTGGAAGAGTTCCAAGGCCAAGAACGCAGCGTCATCCTCATCTCCACCGTCCG AAGCAGCCAGAGCTTTGTACAGCTGGATCTAGACTTTAACCTCGGTTTCCTTAAGAACCCCAAGAGGTTC AATGTTGCTGTGACCCGAGCCAAGGCTTTGCTCATCGTAGTGGGCAACCCCCTCCTCCTAGGCCACGACC CAGACTGGAAAACGTTCCTGGAGTTCTGTAAAGAAAACGGGGGATATACCGGGTGCCCCTTTCCTGCCAA ACTGGACCTGCAGCAGGGACAGGACTTGCTCCAAGGTCTGAGCAAACTCAGCCCCTCTACCTCAGGGCCC CGCCGTCACCAGAATCTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGCCCTTACAAGTGGAGCCAGAGT GGAGAAATGAGCTCTGAGGCCACAGCCGCCCGCCTTCTCACACCAGCCAAGCCTTATTGCTGCCTATCCC TGACCCAGAACCCAGCTCTCCTGTCCCTGCAAGAGATGGGAAGGCCGGGAAGTTTACAGCCCAGACCATT CCGCCCCTTCCTCTGCTAGGGAAGACAACCCAAGCTGCTAAAACTCGGAGGAAGGGGGAGGAAGAAAGCT TTTTTTTTTTTTTAAACCCTCTCCTTTGCAGTGCTGGGGATCGAACCCAGAGCCTCAAATATGCTAGGCA AGCACTCTGCCCCTGAGCTGCTTTAAGAAAAACAAAATGCTGTTCTATGCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC053743
• Insert Size: 3015 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053743, AAH53743
• RefSeq Size: 3535 bp
• RefSeq ORF: 3015 bp
• Locus ID: 17454
• Cytogenetics: 3 F2.2
• Gene Summary: Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (By similarity). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7 (By similarity). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (PubMed:28662698). May function as a messenger ribonucleoprotein (mRNP) clearance factor (By similarity).[UniProtKB/Swiss-Prot Function]