Rbpj (BC051387) Mouse Untagged Clone

CAT#: MC206648

Rbpj (untagged) - Mouse recombination signal binding protein for immunoglobulin kappa J region (cDNA clone MGC:54902, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbpj
• Synonyms: RBP-J, CBF1, RBPjk, Igkjrb
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC051387
  GGCGGGGGGAATCTCGCGAGGGTTGGAGTTTTGGCGAGAGTTTGTGGAAGATGGCGCCTGTTGTGACAGG GAAGTTTGGTGAGCGGCCTCCACCCAAACGACTCACTAGGGAAGCTATGCGAAATTATTTAAAAGAACGA GGGGATCAAACAGTGCTCATTCTTCATGCAAAAGTTGCACAGAAGTCTTACGGAAATGAAAAACGATTTT TTTGCCCTCCTCCTTGTGTGTATCTTATGGGCAGTGGTTGGAAGAAAAAAAAAGAGCAAATGGAACGAGA TGGATGTTCTGAACAAGAGTCTCAACCCTGTGCGTTTATTGGAATAGGAAACAGTGACCAAGAAATGCAG CAGCTGAACTTGGAAGGGAAGAACTACTGCACAGCCAAAACATTGTACATATCTGATTCAGACAAGAGAA AACATTTCATGTTGTCTGTAAAGATGTTCTATGGCAACAGCGATGACATTGGTGTGTTCCTCAGCAAGCG GATAAAGGTCATCTCCAAACCCTCCAAAAAGAAGCAGTCACTGAAGAATGCTGACTTGTGCATTGCTTCA GGAACGAAGGTGGCACTGTTCAATCGCCTTCGGTCCCAGACAGTTAGTACCAGGTACCTGCATGTAGAAG GAGGGAATTTCCACGCCAGTTCACAACAGTGGGGAGCATTTTACATCCATCTCTTGGACGACGACGAGTC GGAAGGAGAGGAGTTCACAGTTAGAGATGGCTACATCCATTACGGGCAGACTGTCAAGCTTGTGTGCTCA GTGACTGGCATGGCACTCCCAAGATTGATAATTAGGAAAGTTGATAAGCAGACGGCATTACTGGATGCAG ACGACCCTGTATCACAACTCCACAAATGTGCATTTTACCTTAAGGATACAGAAAGAATGTACTTGTGCCT TTCTCAAGAAAGAATAATCCAATTTCAGGCCACTCCATGTCCAAAAGAACAAAATAAGGAAATGATAAAC GATGGAGCTTCCTGGACAATCATTAGCACAGACAAGGCAGAATACACGTTCTATGAGGGAATGGGCCCCG TCCTTGCCCCAGTCACCCCTGTGCCTGTCGTAGAAAGTCTTCAGTTGAATGGCGGCGGGGACGTAGCAAT GCTTGAACTTACAGGACAAAACTTTACTCCAAATTTACGAGTGTGGTTTGGGGATGTAGAAGCCGAAACA ATGTACAGATGTGGAGAGAGCATGCTCTGTGTGGTCCCAGACATTTCTGCATTCCGGGAAGGTTGGAGAT GGGTCCGCCAGCCAGTCCAGGTTCCAGTGACTTTGGTCCGAAATGACGGGGTCATTTACTCCACCAGCCT TACCTTCACCTACACACCAGAGCCAGGGCCGAGGCCACACTGCAGCGCTGCAGGAGCGATTCTCAGAGCC AACTCCAGCCAAGTGCCCTCCAATGAGTCAAACACAAACAGCGAGGGGAATTACACAAATGCCAGCACAA ATTCTACCAGTGTCACATCGTCCACAGCAACCGTGGTGTCCTAACTACCGTCTTTTTGCTGGGACTGAAA CTGACTTGAATGCAGCAAAAAGTTGACAGAGAAAGGCAAGGATAAAGTGAACAATCTTTTGTGGTTTCTT GGGGAAACGTTTCGTACCAGGTGATCTATTCAAAACTGCCAGTGCAGGTGTGACATGCAGAGGCCACAGT ACAATGAACAACATCAGAATGCACAGGCTGATGGAAATCCAGGTTTTCAGCTGTCCCTCTCACACACACC CCTTTTTGGTTGTTTTGGTTTGGTTTTGATTTAATGGCCAGGAAACAGATATGTGGCTGGAGCACAGGCT AAGCTAGAAGACACAAACCTAACATAGACTTATGGACCAAGGGACTCGCATAAGCTTTAGTGAAAGGTAC ATTTTCACCATGCCTTTTTTATATCACAGTGTGTACTACACCTTGTTACCAAACAGGTTGCGCTCCCCAC CCTTTGAGGGTTGGCTCTAAAGTACATTCAGGTTCGAGCCTCACCATGTTTGTTCAGTCTGAACACTGAA CACTTCCAGGTTCTTGTGGTCTAAGGCTGGAACTTTTTTAAGCTGAAGTCTTATGACTTTTTCATAAGTT GGAATTATTTTGATTTCAGCAAGTCAAATTTTGTAAAGGCCTGCATATTTTTTTTAAGATTATATGAAGT CTGCAAAAGCTTTAAAAATGCCTCTGCCTTGCCTGCAATACATGCAATGTGTGTTAACTGAGTCTGTTCT CAGAATCTGTTGGTAGTTATTTCTGTGTTTTCCCTTTTTTTTAAAGAAATGTATTTATAGTGGTAATCTA AGAGATGCTAACAGCTCCGTGTGGCCATTTAGCCCTTTCTGAGTTCATGGCAGTGAGCACCGCCGTTCGA AGGCTATGCGCCTCTGCCTGTTCATCAGTTCATCTGTGTTCCAGGATTTGTTCAGGTTTCCCCTTCCCCC AAATCTTGTACATAACTTGTATTATGTGTAAGTTAAACATTTTATCTTTACTTGGAATGTTCCCAGTGAT TACATTTCACAGGGTGTTTCCCACCTTGTTGGCAAATGACAAAAAATATCATGCGAATTATTTGCTACCA AGATGGTGCCCGTAGGGTAAAATGAGGAACATCTCTGCAGGATGATTTATTGTATGTTTTCCTTTTTTTT AGCCTAGCCAGAACATCATTATAATTTTAAAGACCATAAGATGGCTTTTATTAGATGATAACTAAATAAA CTGAACTAAGTAGCCAGAAGACAGTACCTTAGAGACTGATAGTTGTAAGTTTATAAAAATACAAATGTAA CTTATATTTCCATTTTCCTCTTTGCCCCTTTGTTTATTTTCCCCAGTAGAAATGTTCTGTCCAGATTTTT TTTCCCCTTTTTAGTTAAGCCTTGCTTGCATTCAGGCTTGTGTGTCAGCCCTGTGTGCCAATATGCCAGA TTCATTTTGTTTCCATCCCTGCACTTTGGTTATTCCTCGTTCAGTTTCTTGGGAATTGAAGCAGACTCAT TGGGCTACATTTAGTACAAGAACCACATGTGTGATGTTAAAGGACACAGTCTAGTGATGCCATCATCCTT GAGTAAAAACTACCTCTAGATTGTGGTAAGCTTTTACTGTCCCTTAAAACAGGAGCCACTAGTACCTTAC GAATGCAGACTGTAACTTCTATGGCATTTTCTGGTGTCTTTCTGGCTCTCTGGGCTTCTGAAAGTTTTCA CGTAGACCCTTAGACTCCTCTGTCAGGACCATTGCTTTCCAGCGTTGCTGCTTTCACTCGCTGTCTCGCA GACTGTACCACTGTAAGTGCCACTGCGAACATCTTTAAACGGCCTCCTCCTTGGCTTGTCAAGGGGAAGG CCACCTGTAAGATGTGGTGGTGACAGCCTTCCTGTCCACCCCAGGCTGCTCCTTACCAGCTGTTCTAGCC GATTCTCTTCAGATCAGCTACAGCCCACCTCCTCCCCGCCCCGCCTCACCCCTCCAGGCTGTCTCAGTGG TCCAGGGCAGAGCTCACTGTGGCCTTACTGATCTATCTCTGCAGTAACTGTTCTCCCTCAGTTGCGATGG AAGCAGGCCCCTGCGATCTGTTCAGAGCCTGAGCAGTTTGCAGTTCCGTTGTGCTTTCTGAGGTCCTAGA TTCAGGATGTAGTTAAGTCGATAACTGAGAACAGATGAGATGCTTTTAAAGAACAAACTCTTCCTTACCC AGCTTTGTAGCTTAAAGTAAGTTTTCAAGTTCATGGCTTTATTAAAATGAACTTTGACTTTTTAAAATGC TTATATCTCATTTCGGTTCTTTTGTTTATTTTAGTAAGAATTGAAACAGACTATAAGTAGTTTTCCTGTT TGTTTGTTTGTTTGTTTTTTACAAAGTTCAAGAATGTAACAATAAAGGCATTTTGCTATGGTTCTATGTC AGACTCCTACCACAGAATAGTGTGAACAGATATGTCTGTCTGCTTTACCATGTTATTGATAGTTCTTTTG TCAGCTGCTTTTATTAAAAGTCCTCTTTTGTGGAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC051387
• Insert Size: 1464 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051387, AAH51387
• RefSeq Size: 4038 bp
• RefSeq ORF: 1464 bp
• Locus ID: 19664
• Cytogenetics: 5 29.37 cM
• Gene Summary: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations (PubMed:7566092). Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (PubMed:26194095).[UniProtKB/Swiss-Prot Function]