Nckap1l (BC037096) Mouse Untagged Clone

CAT#: MC206642

Nckap1l (untagged) - Mouse NCK associated protein 1 like (cDNA clone MGC:47173 IMAGE:5344383), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nckap1l
• Synonyms: 4930568P13Rik; AI463083; Hem1; Hemp1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037096
  CCACGCGTCCGTCTTGTTATCCGGATGGTCCTCTCTCTCGGTGGCCATCATGTCTTTGACCTCTGCCTAT CAGCTTAAATTAGCAGAGAAGCTCACGATCCTGAATGACCGAGGACAGGGAGTTCTCATCCGCATGTACA ACATCAAGAAGACCTGTTCAGATTCAAAATCCAAGCCACCTTTTTTACTGGAAAAGTCCATGGAATCATG TCTCAAGTACATCAACAAGAAGTTTCCCAACATAGATGTCCGAAATAGCACGCAACATTTAGGACCAGTG CATCGTGAAAAAGCTGAGATAATTAGATTCCTCACTAACTATTACCAGTCATTTGTGGATGTCATGGAAT TTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGCCAGTGTCATTTTGATATTAATCTCAA CTTTGACTTCACTCGGAGCTACCTGGACTTAATTGTGACGTACACCTCAGTCATTTTACTCCTGTCACGG ATTGAGGACCGCAGGATACTCATTGGCATGTACAACTGTGCCCATGAGATGCTGCATGGGCACAGTGACC CCAGTTTTGCTCGCCTGGGCCAGATGGTATTAGAGTATGACCATCCTCTGAAGAAGCTGACAGAAGAATT TGGACCTCACACAAAGGCTGTGAGTGGAGCCCTGCTCTCTCTGCACTTCCTCTTTGTTCGGAGGAACCAG GGAGCTGAGCAGTGGCGCAGCGCCCAGCTTCTAAGCCTCATCAGCAGCCCCCCGGCCATGATTAACCCTG CTAATTCAGACACAATGGCCTGTGAGTATCTGTCCGTGGAAGTGATGGAGCGATGGATCATAATTGGGTT TCTTCTTTGCCATGGGTGCCTCAACTCCAACAGCCAGTGCCAGAAGCTGTGGAAGCTGTGTCTGGAGGGT TCCCTGTACATCACGCTCATCCGGGAAGATGTGCTGCAAGTGCACAAGGTCACCGAGGACCTGTTCAGCA GTTTGAAAGGGTACAGTAAGCGAGTGGCAGACATCAAGGAGAGCAAGGAACACGCCATTACAAACAGTGG CCAGTTTCACTGTCAACGCCGGCAGTTTCTGCGGACAGCTGTGAAGGAGTTGGAGACTGTGTTAAATGAT GAACCAGGCCTGCTTGGTCCTAAGGCCCTTTTTGCTTTCATGGCCCTGTCTTTCATTCGTGATGAGGTCA CTTGGCTGGTTCGCCATACAGAAAATGTCACCAAGACTAAGACACCCGAAGACTATGCTGACTCGAGCAT TGCAGAGCTTCTGTTCCTGTTGGAGGAGATCAGGGCTCTGGTCAGAAGACACATCAAAGTTATTCAGCAG TACCACCTCCAGTACCTGGCCAGATTTGATGTCCTTGTGCTTAGTGACATCATTCAGAATCTGTCCGTGT GTCCAGAGGAGGAGTCTGTCATTATGTCCTCATTTGTTAGTATACTGTCCTCTCTGAATCTCAAGCAAGT TGATAATGAAGAGAAATTTGACTTTTCTGGATTAAGGCTGGATTGGTTTCGCCTACAGGCATATACCAGC GTGTCGAAGGCACCTTTGCACCTGCATGAGAACCCTGACCTAGCCAAGGTCATGAACCTCATCATCTTTC ACTCCCAGATGTTGGACTCAGTAGAGAAAATGCTGGTGGAAACCTCTGACCTTTCTACTTTCTGCTTTCA TCTCCGTACCTTTGAGAAGATGTTTGCTACGACCCTGGAGGAACCAACCATGCTTCGCTACACCATTGCC TTCCCCCTGATTTGTGCTCATTTTGTCCACTGCGTTCATGAAATGTGCCCAGAGGAGTACCCTCACCTGA AGAACCATGGTCTTCACCACTGCAACTCCTTCCTGGAAGATCTGGCCAAGCAGACCAGCAACTGTGTGCT TGAGATATGTGCGGAACAGCGGAACCTGAATGAACAGCTTCTACCTAAGCACTGTGCCACCACCATCAGC AAAGCCAAGAATAAGAAGTCCATGAAGCAGAGGCAGGCTCCCAGGAAAGGAGAGCCTGAGAGGGACAAGC CAGGAGCAGAGAGTCATCGGAAGAACCGCAGCCTTGTCACTAACATGGACAAACTACATCTAAACTTGAC TGAATTGGCACTGGCAATGAATCATGTGCACAGTTTCTCTGTGTTTGAACACACCATCTTCCCATCTGAG TATCTCAGCAGTCACCTGGAGGCCAGGCTTAACAGAGCCATCGTGACTTTGGCTGGCTACAATGCCACAA CCCAGGAGATCCTTAGGCCTTCTGAGCTGCTGGCAGGAGTCAAAGCATACATCAGTTTCATCCAGTCATT GGCACAGTTTTTGGGTGCAGATGCTTCCAGAATCGTTCGCAATGCCCTCCTGCAGCAGACACAGCCACTG GACTCATCTGGCGAGCAGACTGTCACCACTCTCTACACAAACTGGTACCTAGAAAGTCTCCTTAGACAGG CGAGCAGTGGAGCCATTGTCCTCTCCCCAGCCATGCAGGCCTTCATCAGCCTTCCCCGAGATGGAGAGCA GAACTTCAGTGCTGAGGAGTTCTCGGACATCTCTGAGATGCGAGCCTTGGCTGAAATTCTTGGTCCCTAT GGCATGAAGTTCCTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGATTGTGGAGCTGAAGAAGCTTG TGGTGGAAAACATGGATATTCTGGTCCAGATCAGATCCAACTTCAGCAAGCCAGAATTAATGGCATCTCT GCTGCCCCAGCTGACAGGAGCTGAAAATGTGCTGAAACGAATGACCATCATTGGGGTGATTCTCAGTTTT AGGGCCATGGCCCAAGAGGGACTTCAGGAGGTTTTTTCTGCCCACTGCCCTTTTCTCATGGGCCCCATTG AGTGCCTGAAGGAGTTTGTCACCCCTGACACAGACATTAAGGTCACCCTGAGTGTCTTTGAGCTGGCATG TGCTGCAGGGGTGAGCTGTGACATTGACCCAGCCTTGGTAGCTGCCATTGCCAATCTGAAAGCTGATAAC TCATCCCCTGAAGAAGAGTATAAGGTGGCATGCCTACTCTTGATCTTTCTTGCTGTTTCCCTCCCACTCC TTGCCACTGACCCGTCTTCCTTCTTCAGCATTGAGAAAGATGGCTACAACAACAACATCCACTGTTTGAC CAAAGCCATCATTCAGGTGTCTGCTGCCCTCTTCACTCTGTACAACAAGAACATTGAAACGCACCTCAAA GAGTTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGACTGACAAGCTCAAAACCAGAA ATCGTGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCACCCTTCTTGACCCTGGACATGCT GGAGTCCTGTTTCCCTTACGTTCTGCTACGCAATGCCTATCGGGAGGTTTCCCGGGCCTTCTACTTGAAC CGTCTGCCAGCCAGTTCCCACTGAGGGCCCTTTGGAGGTGCCAAGAACCATGCTGCAGTGAAAGTTGCAG CCATTTCTTCAAAAGGGGAGAAGAGTGGGAGGGAGCTCAGGGAGAGACAGGTCAGGCCCCACAGCTGAAA GGGATAAGGAGGAATATCCAGGGCTGAGGAATCATGGAGAAGTGGACAGGCTGGGGCCATGGAGGACAAT TTATAGATAAAGGAGTGTGGAACCACAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC037096
• Insert Size: 3405 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037096, AAH37096
• RefSeq Size: 3686 bp
• RefSeq ORF: 3405 bp
• Locus ID: 105855
• Cytogenetics: 15 F3
• Gene Summary: Essential hematopoietic-specific regulator of the actin cytoskeleton. Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:19015308, PubMed:23424621). Component of the WAVE2 complex which signals downstream of RAC to stimulate F-actin polymerization (PubMed:23424621). Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (PubMed:19015308). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin. Also involved in mechanisms WAVE independent to regulate myosin and actin polymerization during neutrophil chemotaxis (By similarity).[UniProtKB/Swiss-Prot Function]