Anapc10 (BC031460) Mouse Untagged Clone

CAT#: MC206636

Anapc10 (untagged) - Mouse anaphase promoting complex subunit 10 (cDNA clone MGC:25547 IMAGE:3710036), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Anapc10
• Synonyms: 1500026N15Rik; A830003M23Rik; APC10
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031460
  GAGAACCGGAATTGTGGCGAATCAGAAGGATTGTCATGGCATGGTAGAATGTATATTGCTTGTCTCTCTA TAGAATAATTTAAAAATGACCACACCGAACAAGACACCTCCTGGTGCTGATCCTAAGCAGTTGGAGAGGA CAGCAACAGTCAGGGAAATTGGGTCTCAGGCTGTTTGGTCACTCTCTTCCTGCAAACCAGGGTTTGGAGT GGATCAATTACGGGATGACAATCTAGAAACTTACTGGCAATCTGATGGCTCTCAGCCTCACTTAGTGAAC ATCCAATTCAGAAGAAAAACAACAGTGAAGACATTATGTATTTATGCAGACTATAAGTCTGATGAAAGCT ACACTCCAAGCAAAATCTCAGTCAGAGTAGGAAATAATTTTCACAACCTTCAAGAAATCCGGCAACTTGA ATTGGTGGAACCAAGTGGCTGGATTCACGTTCCTTTAACTGACAATCATAAGAAGCCAACTCGCACATTC ATGATACAGATTGCTGTTCTAGCCAATCATCAGAATGGAAGAGATACCCACATGAGACAAATTAAGATAT ACACACCAGTGGAAGAGAGCTCCATTGGGAAATTCCCTAGATGCACAACCATTGATTTCATGATGTATCG TTCAATACGGTGACTTTAAAATGGACAGAAGTGAACAGACGTTTGTTTTATCCTATCATTGGATAGTATA TCAAGTATCTTTGGTGAAAAGTCATGTTACTTTGCAATTTTACATACATTTAAAATAATTTAGATAAATA ACTTTTAGGTCATGTCTGTTTTTTATATAATAAAGCTCATATTTTATGTTGCTAACATGAATTTGAAGTC AATCTTTAAATTTTTCTTTATATTAAAAGAAAACTAGTTAAACTGAAAATTTCAAGATATATATACTATG TGTTTACTTTATTTATGGTGGTTATTTCATATAATGGTTGTTGGATATTGGGAGCAAAATGGAATTTTCA ATAGCACTGAAGTGGAAGCTTAAAGGTACTTTGGAATATTAGTTGTGTTGGATGGTGTTTTCCTGGAGCA GACACAGGTGAAAGACCAAGGAACATTTGGCTGAAACAGACCCAGGTATGTTCTGCTAAAGCAAGCATGT GAGAGGGCTCTGAGTGAAGACATTCATGTATTGGTCCTCCTTACATTGCATAGTTGAGCTGCATTTGTTG GGACTCTTAACACCAAAACCTTCTGGTGGTGTGCTACTGCTTCTTGTTGCTTCTGCAGATTTGGGCTGAT TGGCAGAGACCTGTCACCTGAGACATACTTAATGTGCTGAAGCAAAGCATGTGCTGAGACAATAACATGT GGAGGACACTTGATGTCTGGAGAGATGATAAATAGGACTCACAATGACGGAGGCTGAACTAGTCTTGCTT ATAGAGCTGTCTGTGCAACGCTTGAGAGGCACAGCTGAGAGCTTTTCCTGGTGTTCCTGTTGGCCATGGT CCTTCCTGTTGACTAGTGCCAGAGCTGAGGCCTGGCTGTCTCTGCTAGGTAGTGCCATCGCTGCTGATTG GTGTTTGCTATCCTGACTTCTGTGAACTGAACTGCTGATTTCCTGACAATGCAGATGGGAGTTGCTCTAA AGAACCATATCTTAAACAAATCTACTTCCCCTGTGTTCTATCCTTCCACTACTTCTGGTGGATGGAAGGC TAAAAGGAAGGTTAAAGCATTTAAGAACCCTCATTTAAAAAAAGGCTTGAAAAAATTAAAGTTACACATT AATTTTTTTTTTAGAGGAAATTAAATGTTTGTGTCCCCCCTCCCCCGAAACAATGATAATTCAATCTCTG GGTGCAACAACTAGAATCCTAATCTTGTAAGCCATATTAAATCTAAATCCTCAGGTGGCAAATCCTGGCA GATTCGCCATTTAAGCTGAGGACACTAGTAGCTACATCTTGCCTTCTCGATAACCCTGTCCAAAAAGCCC CTAACTCTCCTGCTTCCTCTCTTCCTCTGTCCAACCTTGAAGTCTCACCTACTTGCCCAGTGATTGGTCC CTGTATTCAGACAGCCCCTCCTGGGAAAGCAGAATTAACATCGTAAGTGGAATTAATACAGGCATCACCA GGGCCATCCACAACACTTTCCTTTTTCTGTACTAATAAAAAACAAAACTTTTCTCTCAAATATAAATTGA GCACAACTACTACCATTTTGTAATTTATAAAGTACAAGATAGACCTAACACCCAGTCAGTCCATCATTTT TGTCAATTAAATAGAACACTGTCATATTTCCTAACTTAAAAGACGTATAATTCTACACCTGACTTATGTC CTGGCTTTAGATTGTATGCCATCTGAAAACCATCCTCAAATCTGTTTTATCAATGTTAAATAGCCTGGGT TGGCTTTGAGACTATAAGTAGTCTTTCAACTCCATCAGAAATCCGAGAATGACCAACATTATCTGAAGAT AAAGGAAGCCTAACACAGCTTCCAGACCTGAGACAAGTTGTAGAGACAGCTGCCTACCCATATAGCCCCA GTAAGTCAGGAAGTTGGAGCATCAATCTTCATCCTCTGGCCCAGAATCATCTGACAGATCTTTGAAAAAC AGGAATTGTTAAGGATTAGCCTATTGTCTCAGCAGAACTAAGCTGTCCTAACCTGAACATTGTGTCCTTA GATAGCCAGTTTCTGCTCAGTGGCCACCTGGCCACAGTGTATCACTTCACTTGGAGGTATAGATGCTCTG TTGCTTTGAATCCATGAATGAGAAGCTGTTAGGAGATTATCTCAGCAAGTGAATAAATAACATTAAATGT CAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC031460
• Insert Size: 558 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031460, AAH31460
• RefSeq Size: 2821 bp
• RefSeq ORF: 558 bp
• Locus ID: 68999
• Cytogenetics: 8 37.79 cM
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]