Thtpa (BC025562) Mouse Untagged Clone

CAT#: MC206627

Thtpa (untagged) - Mouse thiamine triphosphatase (cDNA clone MGC:38198 IMAGE:5322971), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thtpa
• Synonyms: MGC38198
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025562
  CCACGCGTCCGCGGACGCGTGGGCGGACGCGTGGGGGAAAAATGCAGGATGAACAGCGGGCAAGAGGAAG CTCTCTCTTCTCTTGCCCACCCGGTTGGGTCGCACGTAACCTAACAGACCTCTCAGGCCTAGCTTGTCAT CGGCACCGCCACACCGGTCTAATATGGCCGCTAGTGCTGGGACCGGTCTCGCGCGTAAAGAGCCTGGACT AAGGTCCGGAGCCGGAGACCGCTCCCGGCATTCCTCGCGGGTGTAGCGTGGGCACCCTTCCCCCACTTCC GGGTCCCGGGAGAAGGAGACCCTGGGGTCTTGAAGAGTGTCCCGAGGTACCGCTGTTTGGTGCTAGGCGA AGAATGGCAGATCATCCTGGGCAGTGAATCACTGGCCTTGGGTAGCAAGGTGTAGCGAGAAGATAGGTCA TCAGCATTACGGCCTGTTTGCTAGGGAGCCTTTTGGGTTATCAGTAGTAGGATTTAACTGGAGACTTGTC ACCATTGTTGCAGCCCGCAGAGACCTCCAAGTAGAGCCCTGTGAACCTGGAGCAGAGCTCTGGGACAGTG TATATGCATCTTAAATTGATCCAGGACTAGTCCGTCTTTGCTGACTAGTCCTCACCTTGAGCACTCAGTG AATACCTGGCAAACAGGGTTGCAGGTTAATATGGCCCAGGGCTTGATTGAAGTGGAGCGAAAGTTCGCTC CAGGGCCTGACACGGAGGAAAGGCTGCAGGAGTTGGGGGCCACCCTGGAGCACCGGGTCACCTTCCGAGA CACATACTATGATACCTCTGAGTTAAGCCTTATGCTGTCTGACCACTGGCTGCGACAACGAGAAGGTAGT GGATGGGAGCTCAAATGTCCTGGAGTAACAGGTGTCTCAGGACCTCACAATGAGTATGTGGAAGTCACAT CGGAAGCTGCGATTGTGGCCCAGCTCTTTGAACTGCTCGGGTCTGGGGAGCAGAAGCCTGCAGGTGTGGC TGCTGTCCTGGGCTCGCTGAAGCTGCAGGAGGTGGCTAGTTTTATTACTACGCGAAGCTCCTGGAAGCTG GCCTTATCTGGAGCCCATGGACAGGAGCCTCAGCTCACAATAGACTTGGATTCAGCGGACTTCGGCTATG CTGTGGGTGAGGTAGAGGCCATGGTGCACGAGAAGGCTGAAGTCCCGGCTGCCCTAGAGAAGATCATCAC CGTCAGTAGCATGCTTGGAGTGCCAGCACAGGAGGAGGCACCAGCCAAGCTCATGGTATACCTACAGCGC TTCAGGCCTCTGGACTATCAGCGCCTGCTAGAAGCAGCCAGTTCTGGTGAGGCCACAGGGGACTCAGCAT CCTGACAACAGCGTTCACTTTCCAAAAGGGAAGGTAACTCTGAAGCCGCCATTCACATCCGGACCCACGG TGCTCCTTTCCCTTACAACGGAGCCACTTTCCCCTCCAGCTCTGCCTGGTCTTTCGCCCACTCTGAGCTA TCCTTCCTGGAGCCCTCCCTGGCTGCTTCCCTCATCCGTCAGATGCAATCTGTGGGCCGCCCCTCTCCCC TGGCCGCTAAGCAGCCTCCATTGATTTCCGCTCGTGTTTATAGGATTTCCACTTAGCCGTGATCAGTAGT TAAGCACAGGAAAATCCCTTGCCACCCCCCCCTCCCTTGGTCGATGCCATTGATTCTGCCAGAGGCTCTG AAGCCGCCTTACAGCTGAGTTAGAGATGAGGGGAAACAGCGGGGATTTCCCTGCCCTGGGGTGTAGGGAA GAGCAAGAAGGTGGGGAAAAGGTTAGGAACTCCTTAAGGGATCTAGAAATTCAGGGCCTGCTGCTGTGCC GGGAGGGTAGAGTGCTCAGTGTGGCAGTTTAGAGAGAACATTTGCTCTCCAACCCAGAGAGGGGTAGAAA GAAAATGGTCATTGTTGAGACCGGTTCCTAGGGTTTCAGGAAGTCATACTTCCTGTGGAGAAGGCACCAC CCTTGCTCTTAACACATTTTTTTTTCTCTCAACTAGGTCAAAACAGGCCTCTCTTCCCTTCTCACGCTTC ATAGACTTTATTTGTTGGGGAGGAGGCAGGCTCTCTATATTGCCTTGACAAATATAGGGTCTGAGTGCCA CCTGCTGGTGGCCCTCAGGGTTGTGTCCCAAGCTGAGGGTGCAGAAAGATTTGAGAGGAGCCGAGGCAGT TATGAGGCAGGCAAGCTTAGGGAACAGGTTAGCAGCCTGGAACTCCACTTGGTTTGAGCTATGCACACCT CTCATCTCACTGCCACTTGGCATCTTAGGACCCTCAGCTGCACGCACCATGCACACACATTCTTCTAACA GTGCAGCCAGGACCCCGCCTGTATCGAGTAGCACTTAGAGCCCACTTCAGACAGTAAATCTCTCTTTCCC CAGCAGCCATCAAAACTCCCCTTCTGTGGACAGTTTTATTCAAACACTTTAGGACATACCCCAAAGTAGG GAGCTGATTAGGCCTAAACGGGTTTGGGGTAACCTGTGATCTTTGGGTGACCTTTTGAGGAGAACTCCCT ATGGACCGTCCTATGCCTGTGCAGAATGAGTTAGCTTATGATTAAAGTCAGCTGCATCATGGGAAGGGTT ACGCACAGAGAAGAAAGGCTGACAGAATCCAAGCCTGTCAATGAAAACAGAGAATCACTCAAATTATGCC ACTTCACAGCTAACACCTTCGTTCCCTGAGACTTTATTAAAGGAACACCGGGTGACTGCCCTGGGCTCTT AAGACTGTGGTCATCACCCGTTTACAGAGCACTTCTAATTTGTACGCTCAGTTTGCCTTTAAATAAAGTT TACTTCGCCAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC025562
• Insert Size: 675 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025562, AAH25562
• RefSeq Size: 2824 bp
• RefSeq ORF: 675 bp
• Locus ID: 105663
• Cytogenetics: 14 C3
• Gene Summary: This gene encodes a cytosolic enzyme that catalyzes the conversion of thiamine triphosphate to thiamine diphosphate. The encoded protein requires divalent cations for enzymatic activity. The gene product is believed to play a role in regulation of thiamine triphosphate intracellular concentration, and it may be a messenger in cell signaling in response to cellular stress. [provided by RefSeq, Mar 2010]