Med28 (BC024941) Mouse Untagged Clone

CAT#: MC206596

Med28 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 28 homolog (yeast) (cDNA clone MGC:28341 IMAGE:4017146),, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med28
• Synonyms: Eg1, magicin, FKSG20
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024941
  CGGACGCGTCCGCCCACGCGTCCGGCCCGCCGTTGCAGACATGGCGGCGTCCCTGGGAGGGATGTTCACT GGGCAGCCTCCAGGTCCTCCGCCGCCGCCGCCCGGGCTCCCAGGCCAGGCGTCGCTTCTGCAAGCAGCCC CTGGGGCCCCGAGACCATCTAACAGCACTTTGGTGGACGAGTTGGAGTCATCCTTCGAGGCTTGCTTTGC TTCCCTTGTGAGTCAGGATTATGTCAATGGGACTGATCAGGAAGAAATTCGAACTGGTGTTGATCAGTGT ATCCAGAAGTTTTTGGACATTGCAAGACAGACAGAATGTTTTTTCCTACAAAAAAGGTTGCAGTTATCTG TCCAGAAACCAGATCAAGTTATCAAAGAGGATGTCTCGGAACTAAGGAGTGAACTGCAGCGGAAGGATGC GCTGGTCCAGAAGCACTTGACAAAGCTGAGGCATTGGCAGCAGGTGCTGGAGGACATCAATGTGCAGCAC AAGAAGCCGGCTGACATGCCTCAGGGATCCTTGGCCTTCCTCGAGCAGGCGTCTGCCAACATTCCTGCAC CTCTGAAGCAGACCTAAGCACTCCTGCCTGTCTTGCAGCCTTGCAGTCTTGCCAGACACGTTTCCTTACA GACCTGGTGTGTTGGAAGGACTCTGTTGGATAATGAGTTTGTATCAGTTTCTGTCCCTATTTATAAGGTA GGGTTTCATTATGAAGCCGGGAAGGGCCTTGAATCTATGATCCCCTGCCTCAGCTCCTCTAGTGCTCTAA ACTCCTGAATACTTTATAAAAAACCTCTGTCTTTGATAAAGTAAAGTTTGGCTTTGTTTGTTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC024941
• Insert Size: 537 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024941, AAH24941
• RefSeq Size: 904 bp
• RefSeq ORF: 537 bp
• Locus ID: 66999
• Cytogenetics: 5 B3
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function]