Ralgds (BC027225) Mouse Untagged Clone

CAT#: MC206585

Ralgds (untagged) - Mouse ral guanine nucleotide dissociation stimulator (cDNA clone MGC:27833 IMAGE:3486732), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ralgds
• Synonyms: Gnds, mKIAA1308
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027225
  CCCTGGGGCTGGCGATGAGCTGCCGAGGCTGAGGATGATGGTAGATTGCCAGAGCTCCACGCAGGAGATT GGGGAGGAGCTGATCAACGGGGTCATCTACTCCATCTCCCTGCGCAAGGTCCAGCTACACCAAGGAGCCA CTAAGGGCCAGCGCTGGCTAGGGTGTGAGAACGAGTCGGCTCTGAACCTCTATGAGACCTGCAAGGTGCG CACGGTGAAGGCTGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTT TCCTACGTCACTGTCTTCCTGTGCACCTACAGAGCCTTCACTACCACCCAGCAGGTGCTAGACCTGCTGT TCAAAAGGTACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATATGGCTGCATCCTCCCCTACTCCAG TGAGGACGGCGGACCGCAGGACCAACTCAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAA TACTCAGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCTTATGTACAGCTCA ACATGCCTGGCTCAGATCTGGAGCGCCGCGCTCACCTTCTCCTGGCCCAGCTGGAGGACCTGGAGCCCAG TGAGGCTGAGTCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCTGAAGCCAGCTTCACAGCTAGAACCT GCACTGCTGCTGACGCCCAGCCAAGTGGTGACATCAACTCCAGTACGAGAGCCCGCTGCGGCCCCAGTGC CAGTGCTGGCCTCCAGCCCAGTGGTGGCACCAGCTCCTGAGCTAGAACCAGTTCCAGAGCCACCTCAAGA GCCTGAGCCATCCCTAGCACTGGCTCCAGAGCTGGAGCCCGCCGTCTCACAGAGCCTGGAACTGGAGTCA GCTCCTGTGCCCACTCCTGCCTTAGAGCCTTCCTGGTCTCTGCCTGAAGCCACGGAGAATGGACTAACCG AGAAGCCTCACCTTCTGCTGTTCCCTCCTGACTTGGTGGCTGAACAGTTTACTCTGATGGATGCAGAACT ATTCAAGAAAGTCGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAACGGGACAAGAAGGGCAAGGAG CACCTCGCGCCTACCATCCGCGCCACTGTTGCCCAGTTCAACAACGTGGCCAACTGTGTCATTACTACCT GCCTTGGGGACCAGAGTATGAAGGCTCCGGACAGGGCCCGGGTGGTGGAACACTGGATCGAGGTGGCCAG GGAGTGCAGAGCGCTCAAGAATTTCTCCTCCCTCTACGCCATCCTCTCTGCTCTACAGAGCAATGCCATC CACCGCCTAAAGAAGACGTGGGAAGAGGTCTCCAGGGACAGCTTTCGAGTGTTCCAGAAACTGTCGGAGA TCTTCTCTGATGAGAACAACTACTCCCTGAGCAGAGAGCTGCTCATCAAGGAAGGAACCTCCAAGTTTGC CACACTGGAGATGAACCCTAGGAGAGCCCAGAGGCGGCAGAAGGAGACAGGAGTCATCCAGGGCACCGTT CCCTACCTGGGCACATTCCTCACTGACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTCTATGGGA GACTGATCAACTTTGAAAAGAGAAGGAAGGAGTTCGAAGTCATTGCCCAGATCAAGTTGCTACAGTCAGC CTGCAACAACTACAGCATTGCTCCGGAAGAACACTTTGGAACATGGTTCCGAGCTATGGAGCGACTCAGT GAGGCTGAGAGCTACACCCTGTCGTGTGAGCTGGAGCCCCCGTCTGAGTCGGCCAGCAACACCCTGAGGA GCAAGAAAAGCACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCTCCCAGCACGGAGCTCAGCACCAG TAGCAGTGCCCACTCCAAGTCCTGTGACCAGCTTCGGTGCAGCCCTTACCTCGGCAGCGGGGACATCACC GACGCGCTCAGTGTGCACTCAGCTGGCTCTTCCAGCTCTGATGTGGAGGAGATCAACATGAGCTTCGTCC CAGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCTCCCAGTCGTCCCCAGAGACCTCTGG CATCAGCTCCGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCCACCACGCCCGTGTCTACCACGCGCACC CACAAGCGCTCCGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTGCCTCTCTACAACCAGCAGGTGGGCG ACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACATGTACAAGAGCATCCTGGTGACCAG CCAGGATAAGGCTCCGACTGTCATCCGAAAAGCCATGGACAAACACAACCTAGATGAGGACGAGCCAGAG GATTATGAGCTGGTGCAGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCT ATGCCATGAACTCTACCGCCAACTATGACTTCATCCTAAAGAAGCGGACATTCACTAAGGGGGCTAAAGT CAAGCATGGAGCCAGCTCCACCCTCCCTCGTATGAAGCAGAAGGGACTCAGGATTGCCAAAGGCATCTTC TAAGGACGTCTCCTGGGACGGGCTGACTGGCTGGGGACTAAGCACTTACAGACTAGAGTGGCCCTGGCCA CACAGGTACCTCTGCCCACCTGCCAGTCCAGGCTACCCCCCTACTCCACTTTCACCCTGAACCTCTCCTG CTGCCGGGATTGACACCTGCCACTGACAGGCTGACCTGGCCTCTGGGGACCACTTGCCGCCTTCGGTGCC TTCTGCTCTCTGGAACCAGAGGACTAGCTGACTTTTGCCAAGGAGTGTTGCCAACTGGGCATGGGACCTT GCCTGCCCTGGACACTATCCACCACACTTTGCTGACACCTCCCCAGGTGCAGATCACTGCCACCCATGCT CCCGGATGCTCCAGGACACACATACACCCAGCAGGGCTGCCGACTGCATTCTCTCCTTGTGCCCACAGGC ACTGGCCTGGGACCTTCATGGAGGTCCTAGCCTTTCCTCCCACACTCTAGCCTTTCTCAGGCTGCACCAA AGATTCCACCTTCAGGGCCTACAGAGTGAGGGAGCCTCGCCCACCAGGAGCCCAAGCCCTCCCTTGGATC AGAGAGAGAAGCCCTCTCGTGAACTGCCCGGTTGGTATTGAATCCCAGTCCACTGAAAGTGCCATTGACC ACCGCTGCATCCGGGCTGTACAAGACGACACGCAGTGGGAGGTTTGTGGGGAGGAGGAAGACAACTGAAC ATTTGTATAAAACGTAAAAAGTTTACTGATTGGGGTGGGACAATATTTATTTGTTGTAAATAGAAAATGC TAGACTTGAATATTATATTAAAATCCCGTTTCTACTAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC027225
• Insert Size: 2559 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027225, AAH27225
• RefSeq Size: 3412 bp
• RefSeq ORF: 2559 bp
• Locus ID: 19730
• Cytogenetics: 2 A3
• Gene Summary: Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.[UniProtKB/Swiss-Prot Function]