Acadm (BC013498) Mouse Untagged Clone

CAT#: MC206513

Acadm (untagged) - Mouse acyl-Coenzyme A dehydrogenase, medium chain (cDNA clone MGC:19161 IMAGE:4222450), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acadm
• Synonyms: AU018656; MCAD
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC013498, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGCGGCGTTCCGCAGAGGCTGCAGGGTCCTGAGAAGTGTTTCTCATTTTGAGTGTCGAACACAAC
  ACTCGAAAGCGGCTCACAAGCAGGAGCCCGGATTAGGGTTTAGTTTTGAGTTGACGGAACAGCAGAAAGA
  GTTTCAAGCAACTGCCCGCAAGTTTGCCAGAGAGGAGATTATCCCCGTCGCCCCGGAATATGACAAAAGC
  GGGGAGTACCCGTTCCCTCTCATCAAAAGAGCCTGGGAACTCGGCTTGATCAACGCGCACATTCCGGAAA
  GTTGCGGTGGTCTTGGCCTGGGAACGTTCGATGCTTGTTTAATTACCGAAGAGTTGGCGTATGGGTGTAC
  AGGGGTGCAAACTGCTATTGAAGCAAATTCTTTGGGGCAAATGCCTGTGATTCTTGCTGGAAATGATCAA
  CAAAAAAAGAAGTATTTGGGGAGGATGACGGAGCAGCCAATGATGTGTGCTTACTGTGTGACAGAGCCCT
  CCGCAGGCTCTGATGTGGCGGCCATTAAGACCAAAGCAGAGAAGAAGGGTGACGAGTATGTTATCAACGG
  CCAGAAGATGTGGATAACCAACGGGGGAAAGGCCAACTGGTATTTCTTGTTGGCGCGTTCTAACCCAGAT
  CCTAAAGTACCCGCTAGTAAAGCCTTTACTGGATTCATTGTGGAAGCCGACACCCCGGGAATACATATTG
  GAAAAAAGGAACTAAACATGGGCCAGCGATGCTCTGACACCAGAGGAATTGCTTTCGAAGACGTCAGAGT
  GCCTAAGGAAAATGTGTTAATCGGTGAAGGAGCAGGTTTCAAGATCGCAATGGGTGCTTTTGATAGAACC
  AGACCTACAGTCGCAGCTGGCGCTGTCGGGCTAGCCCAGAGAGCTCTGGACGAAGCCACGAAGTATGCCC
  TGGATAGGAAGACATTTGGAAAGCTGCTAGTGGAGCACCAAGGAGTTTCATTTCTGCTCGCAGAAATGGC
  GATGAAGGTTGAACTCGCTAGGCTCAGTTACCAGAGAGCAGCCTGGGAGGTTGACTCCGGTCGCCGGAAC
  ACTTACTATGCCTCGATTGCAAAGGCCTGTGCTGGAGACATTGCCAATCAGCTAGCCACTGACGCCGTGC
  AGATTTTCGGAGGCTATGGATTCAACACAGAGTACCCTGTGGAGAAGCTGATGAGGGACGCCAAGATCTA
  TCAGATTTATGAAGGTACTGCACAAATTCAGAGGCTGATCATAGCTCGTGAGCACATTGAAAAGTATAAA
  AATTAA
  
  
• Restriction Sites: EcoRI-NotI
• ACCN: BC013498
• Insert Size: 1266 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013498, AAH13498
• RefSeq Size: 1857 bp
• RefSeq ORF: 1266 bp
• Locus ID: 11364
• Cytogenetics: 3 78.77 cM
• Gene Summary: This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012]