Mx1 (BC011113) Mouse Untagged Clone

CAT#: MC206496

Mx1 (untagged) - Mouse myxovirus (influenza virus) resistance 1 (cDNA clone MGC:18532 IMAGE:4011821), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mx1
• Synonyms: AI893580; Mx; Mx-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011113
  GCCTGGAGTGTCAAGTGCCTGACCCCAGTGGCAAGGCTTCCAGATTCTTCTGAATCAAACCTGTTATATC CAACTGGAATCCTCCTGGAAAACATACAAAGATGAGTTCTTAAGAACGTCAGAAGGAAAGAGCTGATTTC TTCATCCAGTCACTGGATACCAAGTAAACATCCTGATACCTCTGAGGAGAGCCAGACGATGGATTCTGTG AATAATCTGTGCAGGCACTATGAGGAGAAGGTGCGGCCCTGTATTGACCTCATCGACACCCTGAGGGCTC TGGGTGTGGAGCAGGACCTGGCCCTGCCTGCCATCGCTGTCATTGGGGACCAGAGTTCAGGGAAGAGCTC TGTTCTGGAAGCACTGTCTGGAGTGGCCCTCCCCAGAGGCAGTGGTATTGTCACCAGATGCCCTCTGGTG CTGAAATTGAGGAAGCTGAAAGAAGGAGAGGAGTGGAGAGGCAAAGTCTCCTATGATGACATCGAAGTGG AGCTCTCTGATCCTTCAGAGGTGGAAGAGGCCATTAACAAGGGTCAGAACTTCATTGCTGGGGTAGGCCT GGGGATCAGTGATAAGCTCATTAGCCTGGATGTCAGCTCCCCAAATGTCCCAGACCTGACTCTCATTGAC CTGCCTGGAATTACCAGGGTGGCTGTAGGCAACCAGCCTGCAGACATAGGACGCCAGATCAAGAGACTTA TCAAGACATACATCCAAAAACAAGAGACCATCAACCTGGTGGTAGTCCCCAGCAATGTGGACATTGCTAC CACAGAGGCTCTGAGCATGGCTCAGGAGGTGGACCCTGAAGGGGATAGGACCATAGGGGTCTTGACCAAG CCTGATCTGGTGGACAGAGGTGCTGAAGGCAAGGTCTTGGATGTGATGCGGAACCTGGTGTATCCTCTGA AGAAGGGCTACATGATAGTCAAGTGCAGAGGTCAGCAGGACATCCAAGAGCAGCTGAGCCTGACTGAGGC TTTTCAGAAAGAGCAAGTCTTCTTCAAGGATCACTCATACTTCAGCATTCTTCTGGAAGATGGGAAGGCC ACAGTGCCCTGCCTGGCAGAGAGACTGACTGAGGAGCTCACCTCCCACATCTGTAAATCACTGCCACTAT TGGAAGATCAAATAAATAGCAGTCATCAGAGTGCAAGCGAGGAGCTGCAGAAGTACGGTGCAGACATACC AGAAGATGACAGAACGAGGATGTCCTTTCTGGTGAATAAAATCAGTGCCTTCAATAGGAATATCATGAAT TTGATACAAGCACAGGAAACCGTATCAGAGGGAGACAGCCGGTTGTTTACCAAACTGCGAAATGAGTTTC TTGCTTGGGATGATCATATTGAGGAATATTTTAAAAAAGATTCTCCTGAGGTACAAAGCAAGATGAAAGA ATTTGAAAATCAGTATCGTGGCCGGGAGCTGCCAGGGTTTGTGGACTACAAGGCATTTGAGAGCATCATC AAAAAGCGAGTCAAAGCCCTGGAAGAGTCTGCTGTGAACATGCTGCGCAGGGTCACTAAGATGGTCCAAA CTGCCTTCGTAAAGATTTTATCAAATGATTTTGGTGATTTTTTAAACCTCTGCTGTACTGCTAAGTCCAA AATTAAAGAAATAAGATTAAACCAAGAAAAGGAAGCTGAAAACCTGATCCGACTTCACTTCCAGATGGAA CAGATTGTCTACTGCCAGGACCAGGTTTACAAGGAAACCTTGAAGACGATCAGAGAGAAGGAAGCTGAGA AAGAGAAGACCAAGGCATTAATAAACCCTGCTACCTTTCAAAATAACTCTCAGTTTCCTCAAAAGGGGTT GACTACCACTGAGATGACCCAGCACCTGAAAGCCTACTACCAGGAGTGCAGACGGAATATTGGGAGACAG ATCCCTCTGATTATCCAGTACTTCATCCTGAAAACATTTGGGGAGGAAATAGAGAAAATGATGCTTCAGC TTTTACAGGACACCAGTAAGTGCAGCTGGTTCCTGGAGGAGCAGAGTGACACCAGAGAGAAGAAGAAGTT CCTGAAGAGGCGGCTTTTAAGGCTGGATGAGGCTCGGCAGAAGCTTGCCAAATTCTCCGATTAACCAGGC TAGCTATGTGGCTTTCCTGGTCGCTGTGCAATGATCCTTTAGCTGCTAACCTTATGTCAGAATGTTGCCT TTAGACTGTGGAAGCAAGCAAGCCCTTTGTAAACTCTGAGGAGATGAGGAGAGAGGAGCTATGGAGAGTC ATTCTACTTCAGCAGGAAGACATGACCCTCTCAAAGCCTAGGAGGATGGCACCTCTCAGGATCCTGCTCC TAACTGTCTTTTCTTTGTCAGGAGGATGTACTGGCTGAGCACCTCAGAAGGGTCGGCTTCTGGTTTTGTA CTGTCCTGTCTGGAAGCAGTGCGAGAGGTGCCACAGGGTCCTGGTAGGACAGCTCTTTGTAGGCATACAT TTTTTTTTGCAAAAATTTTAACAAGGATTCCACCTCTCTTTTAGCCCACCACCCATCAGAGGTAGTGGAA GAGAAAAGTTATTAGGCTATGGAGGAAGTGGACCTGTTCAGCAATAGTTCTTTGGGGGCGAGCTCAGTCT TCCTTGGCAGCAGTTCAGTCCCATAACAAACACCAAGTATGACTCAGCAGCTGCAGACCAGTCCTCTAGG CAGGCAGACACCACATACAACCAGCAGCTATAGTCCAGTCCTTTCCACAGGCAGAAACCAAGAATGAATC AGCAGCTGAAGTTCAATCTTGATGAAAGCTTGCCATCTAGCCTGAGGTGAGGCTGCAGAAGCAACAAACT GCTGCAGGATCCTCACAAGTAGTTCTTAGGTGAGTTTCTCCCAATGTCGGTGTTACCACAAGTGGAGCTC AACCATGCTATGTAAGGCAAACCATTCCATGCATGTTGATAGTAAAGAATAGCTAGACAGAGCAAACCAA GCCAAGGCTCCGTTGCTCATCTCCGACTGTTTGTGAGGTCATATTTATACTCCTTCATCATGGGTCCTTT CATGTATTTGCAATGTCATATGAAAGCATCCTTTCAGCTGTGTCTGCTTCAGGGAAACATTCTTTCATGT GTTTGCTTCAGCAAGACATCATTTCACCTGTGTACCCCAGCAAAACATCATTTGATATAACTTTCCAAGG AAACTGGAAGTTTCCACTTTAGCGCTTCCAATAAAATTCTTGCTAAGTGCCAAAAAAAAAAAAAAAAAAA AAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC011113
• Insert Size: 1896 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011113, AAH11113
• RefSeq Size: 3224 bp
• RefSeq ORF: 1896 bp
• Locus ID: 17857
• Cytogenetics: 16 57.46 cM
• Gene Summary: This gene encodes a member of the Mx protein family of large GTPases, and functions in the innate immunity system. Interferon alpha/beta treatment or viral infection induces expression of this protein, which subsequently accumulates in the cytoplasm and inhibits viral replication. It has been shown to confer resistance to the influenza virus. This gene produces a functional protein in some feral mouse strains, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a deletion or a nonsense mutation that results in a non-functional gene product. [provided by RefSeq, Aug 2015]