Pycr2 (BC006882) Mouse Untagged Clone

CAT#: MC206479

Pycr2 (untagged) - Mouse pyrroline-5-carboxylate reductase family, member 2 (cDNA clone MGC:6271 IMAGE:2647612), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pycr2
• Synonyms: 1810018M05Rik; P5cr2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006882
  CCCACGCGTCCGCTCTGGGATACTTGGGGCCTCTCTAGCAGTTCATCTGGTGCATCCCGGTTTGAAAGCT GTTCCGAGCAATTCGGGTCCATCTTTTTCCACGCGCGGACACTGACCCCGGGATCCCCAGAGTCCCCGGA TCGGCGCTCGCCGGGATCCTGCGGCCATGAGCGTGGGTTTCATCGGCGCAGGCCAGCTGGCCTGTGCTCT GGCGCGGGGCTTCACGGCCGCGGGCGTCCTGTCGGCTCACAAGATAATAGCCAGCTCCCCGGATATGGAC CTGCCCACGGTGTCTGCGCTCAGGAGGATGGGTGTGAATCTGACCCGAAGCAATAAGGACACTGTGCGGC ACAGCGACGTCCTGTTCCTGGCTGTGAAGCCCCACATCATCCCCTTCATCCTGGATGAGATTGGAGCCGA CGTGCAGGAGAGGCACATCGTGGTCTCCTGTGCAGCCGGGGTCACCATCAGTTCTGTGGAGAAGAAGCTG ATGGCTTTCCAGCCGGCCCCCAAAGTGATCCGCTGCATGACCAACACGCCCGTGGTGGTCCGAGAGGGGG CCACCGTGTATGCCACGGGCACCCATGCTCTGGTGGAAGACGGCAAGCTTTTGGAGCAGCTGATGAGCAG TGTGGGCTTCTGCACAGAGGTGGAGGAAGACCTCATTGATGCTATCACCGGGCTCAGCGGCAGCGGGCCT GCCTATGCATTCATGGCCCTGGACGCCCTGGCAGATGGTGGGGTGAAGATGGGCGTGCCGCGGCGCCTGG CGGTCAGACTGGGAGCTCAGGCGTTGCTGGGAGCAGCTAAGATGCTGCTGGACTCAGAGGACCATCCAGG CCAGCTCAAGGACAACGTCTGCTCCCCTGGGGGGGCCACCATCCATGCCCTGCACTTCCTGGAGAGTGGG GGCTTCCGCTCTCTGCTCATCAATGCGGTTGAGGCCTCCTGTATCCGAACCAGAGAACTGCAGTCCATGG CTGACCAAGAAAAGGTGTCTCCTGCTGCCCTTAAGAAGACCCTTCTGGACAGAGTGAAGCTGGAGTCACC CACGGTGTCCACGCTGGCCCCACCCAGCTCAGGGAAACTCCTTACAAGAAACCCCGCCCAGGGAAGCAAG AAGGAGTGAGGAGGACTCTGTAGGGTGTCCCTGTCCCCTGTAGTACAGGGAGGGGCAGGCTCAGGTCTGG GGGTGAAGCTGCTTACATCTGTTCACCTGGTGCCACCGAGTCCTTCTCCTGTCCCGTGGCAGAAGGTGCT CTGAAGAGGCGGCTGATGCCAGAAGTCAGCATGCCTCTCTGAGAGAGGTGGAGGCCTTAGAGTTAGCATC CCCCTCAGCGGAAGTCTGTGGAGAGCTTTAGTTAGATCCGAGGCTGCTTCAGATTGATCTCCAGGTGAGG TGAGAGGAAACGGGAATCCTCTGAATTAAAAACACGCAAAGAAGCCTGTGTCTTGGTGTTGCAGGTGGAA GTGTGGGGAGTCATCCTGTGACCTCTTGCTTAACTCTTCTGGACAGGTGACCCATTAGAAGGTGATGTTC AGGGCTGCTTTAATAAAGATCTTATTTATTTTTGAAAAAAAAAAAAAAAG
• Restriction Sites: EcoRI-NotI
• ACCN: BC006882
• Insert Size: 963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006882, AAH06882
• RefSeq Size: 1590 bp
• RefSeq ORF: 963 bp
• Locus ID: 69051
• Cytogenetics: 1 H4
• Gene Summary: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (By similarity). Involved in cellular response to oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function]