Il1a (BC003727) Mouse Untagged Clone

CAT#: MC206467

Il1a (untagged) - Mouse interleukin 1 alpha (cDNA clone MGC:5780 IMAGE:3599550), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Il1a
• Synonyms: Il-1a
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003727
  GCCACTGGTGTTCTCTTACAGAAATCAAGATGGCCAAAGTTCCTGACTTGTTTGAAGACCTAAAGAACTG TTACAGTGAAAATGAAGACTACAGTTCTGCCATTGACCATCTCTCTCTGAATCAGAAATCCTTCTATGAT GCAAGCTATGGCTCACTTCATGAGACTTGCACAGACCAGTTTGTATCTCTGAGAACCTCTGAAACGTCAA AGATGTCCAACTTCACCTTCAAGGAGAGCCGGGTGACAGTATCAGCAACGTCAAGCAACGGGAAGATTCT GAAGAAGAGACGGCTGAGTTTCAGTGAGACCTTCACTGAAGATGACCTGCAGTCCATAACCCATGATCTG GAAGAGACCATCCAACCCAGATCAGCACCTTACACCTACCAGAGTGATTTGAGATACAAACTGATGAAGC TCGTCAGGCAGAAGTTTGTCATGAATGATTCCCTCAACCAAACTATATATCAGGATGTGGACAAACACTA TCTCAGCACCACTTGGTTAAATGACCTGCAACAGGAAGTAAAATTTGACATGTATGCCTACTCGTCGGGA GGAGATGACTCTAAATATCCTGTTACTCTAAAAATCTCAGATTCACAACTGTTCGTGAGCGCTCAAGGAG AAGACCAGCCCGTGTTGCTGAAGGAGTTGCCAGAAACACCAAAACTCATCACAGGTAGTGAGACCGACCT CATTTTCTTCTGGAAAAGTATCAACTCTAAGAACTACTTCACATCAGCTGCTTATCCAGAGCTGTTTATT GCCACCAAAGAACAAAGTCGGGTGCACCTGGCACGGGGACTGCCCTCTATGACAGACTTCCAGATATCAT AAAAGCAGCCTTATTTCGGGAGTCTATTCACTTGGGAAGTGCTGACAGTCTGTATGTACCATGTACAGGA ACCTTCCTCACCCTGAGTCACTTGCACAGCATGTGCTGAGTCTCTGTAATTCTAAATGAATGTTTACCCT CTTTGTAAGAGAAGAGCAAACCCTAGTGGAGCCACCCCGACATATGATACTATCTGTTATTTTAAAGAGT ACCCTATAGTTTGCTCAGTACTAATCATTTTAATTACTATTCTGCATGGCATTCTTAGGAGGATCAAAAA GACTCTACACATATTACAGATGGGTTAACAAAGGGATAAAACAACTGAAAAGCACACTCAATGCATTTGG AATATAAATTCACAGACCAATCTCACTGTGCACCTTCGGCTTCAAAATGCCAGTTGAGTAGGATAAAGGT ATAAGAACTTAATGCTGTCATTTTCAAAAGGAAGGGGACAATAGCTACATCTTTCCTACCTCAGTGGGTT TTACTCCAGTGAGATCATTTGGATGAAATCCTCCTGTAACAGACCTCAAGAAGGAGACAGACTGTTGAAT GTTATTTTTAAGTTATTTTATATATGTATTTATAAATATATTTATGATAATTATATTATTTATGGAACAT CCTTAAATCCTCTGAGCTTGACAGGCATCCTCACAGCAGGATTTTCTAGGTGGTCAGTTAGATATAGTTT CCTCTAGAGCACCATGCTACAGACTTTACACTTTTTCCACAGCCACGAAGCTCTCTGTACATTCCTGTAC TTGGGAGCCCTTTCATCATGATCTTAATCTGTACTGTTTACTTTGTTCATCTAAAATGATAATTGAGTCA GTCTTTTTCCCTCCCATCCTTAAAGCTGTCTGGGTATTCTTACATCATTCAGTCTCACCTGTAACTAACA CCAACCATCTAAAGATGGAAAGAGCTTAACTGTGACAACCACATCACTGTTACCTGAAGTTTCTTTTCTA GAATGTAATCAGTGTTTCCCCTGGATTCCAATATTTTTTTCAAACCACAGTATCATGTAACTATCAACAA TAACAATCAACTCATTATTATTAATCATAATTAAATAAAACAAGTTTGAGCTGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: BC003727
• Insert Size: 813 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003727, AAH03727
• RefSeq Size: 1973 bp
• RefSeq ORF: 813 bp
• Locus ID: 16175
• Cytogenetics: 2 62.9 cM
• Gene Summary: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[UniProtKB/Swiss-Prot Function]