Hibch (BC026437) Mouse Untagged Clone

CAT#: MC206437

Hibch (untagged) - Mouse 3-hydroxyisobutyryl-Coenzyme A hydrolase (cDNA clone MGC:31364 IMAGE:4238681), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hibch
• Synonyms: MGC31364
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026437
  GTCTAACTACCAGAGCCGGTGGAACCACTACACTCTGCTTCTTCTGCTCTTGGGTTTTTAGGCCATGGGG CAGCCATATGCGTGGCGGCTCCTGTCGAGGGTCAGCTCCTTCAGAAGAGCCAGCGTTATTCTGCAACATC TGAGAATGTCCATGCACACAGAAGCTGCAGAAGTGCTGCTGGAGAGGAGAGGCTGTGGAGGCGTCATAAC GCTCAACAGACCCAAGTTCCTCAACGCACTGAGTCTGAATATGATCCGGCAGATCTATCCACAGCTAAAG ACATGGGAACAAGACCCTGACACATTCCTGATCATCATAAAGGGAGCCGGAGGAAAGGCCTTCTGTGCTG GAGGGGACATCAAAGCACTCTCAGAAGCTAAAAAAGCAAGACAGAATTTAACTCAAGATTTATTCAGAGA AGAATACATCTTGAACAATGCAATTGCATCTTGTCAGAAACCATATGTTGCCCTTATTGATGGAATCACA ATGGGTGGGGGAGTTGGTCTTTCAGTCCATGGTCAATTCCGAGTGGCTACAGAAAGGTCTCTCTTTGCTA TGCCAGAAACGGGAATAGGACTATTTCCTGATGTGGGTGGAGGTTATTTCTTGCCAAGACTTCAAGGAAA ACTCGGTTACTTCCTTGCACTGACAGGATACAGACTAAAGGGAAGAGATGTGCACAGAGCGGGAATTGCC ACACACTTTGTAGATTCGGAAAAGTTGCGTGTGTTGGAGGAGGAGCTGTTAGCCTTAAAGTCACCTTCAG CTGAGGATGTTGCAGGTGTCTTAGAAAGCTATCATGCCAAGTCCAAGATGGATCAAGACAAATCTATCAT ATTTGAGGAACACATGGACAAAATAAACAGTTGTTTTTCAGCCAATACCGTGGAACAGATAATTGAAAAC TTACGGCAAGATGGGTCACCTTTTGCCATAGAACAGATGAAGGTAATTAATAAAATGTCTCCGACATCAC TAAAGATCACACTAAGGCAACTCATGGAGGGCTCTTCAAAGACTTTGCAAGAGGTACTGATCATGGAATA TCGGATAACTCAAGCATGTATGGAAGGGCATGACTTTCATGAAGGTGTTAGAGCTGTTCTCATTGACAAA GACCAGACTCCAAAATGGAAACCAGCCAATCTAAAAGATGTTACGGATGAAGACTTGAATAGTTACTTTA AATCTCTGGGAAGCAGTGATTTGAAATTCTAAAGTTTATTTTGGAGCAAAGGTCAACACATCATAGCACA GAGGCTAGGCCCATTTTCATGCAGCCTACAAGCTGGAAATGGTTTTCTCATTTTTAAATGATTGGGAAGG AAATGAAAAGACTAATATTTCATGATACAGTGGTTGCCCAAAGTTTAAGCACCAATGTTTGTAACTTTTC ATGATATGACAGCTGAGATCCATACACCAATGTTTTATGGAACCTTCCTGCAGCGTAGCAGAGCTTCTCT ATGGCTGCTTGGAGTGGCCATGGCACAGACTTTGACCCACAGCACCTCAAGGGTCTAAGTTTCTACTCCA GTCTTCATCAGAAATGGTTTGTTGGTTCCTGGTTTATAAGAAATTCTCAAGAGCTCCAGAGAATCTCAAG TTTATTTCTCATTAAAATGGTAACTTTGACTTTGTTTATATACTAATTAACCAAATTATGTACAAGTTAG CAAAAATAAAGACCCTGAAATTTGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC026437
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026437, AAH26437
• RefSeq Size: 1720 bp
• RefSeq ORF: 1158 bp
• Locus ID: 227095
• Cytogenetics: 1 C1.1
• Gene Summary: Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function]