Anapc4 (BC024870) Mouse Untagged Clone

CAT#: MC206344

Anapc4 (untagged) - Mouse anaphase promoting complex subunit 4 (cDNA clone MGC:37013 IMAGE:4949079), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Anapc4
• Synonyms: 2610306D21Rik; APC4; D5Ertd249e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024870
  GGCCGCTGGCTAGGAGTAGGCCGAGTCTGCGACTCTGGCAGGTACCGTTGCTGGCAGGGCTTTGTGCCAG AGCTTTGTGGCCGGGTGCTCCGGCGGCCTTCTCGCGCACGTCGTTGCAGGGCCGCACCCATGCTGCGCTT TCCGACCTGTTTCCCATCCTTTCGGGTGGTGGGAGAGAAGCAGCTGCCACAGGAGATTATTTTCTTGGCC TGGTCCCCCAAGCGAGACCTCATTGCTTTGGCCAACACTACAGGCGAGGTTTTGCTGCATCGCCTTGCAA GTTTTCATCGAGTTTGGAGTTTCCCACCAAATGAAAGTACAGGAAAAGAAGTGACCTGCTTGGCCTGGAG ACCAGACGGCAAACTTTTGGCCTTTGCTCTTGCGGATACCAAGAAAATTATTTTGTGTGATGTAGAAAAG CCTGAAAGCTTACACTCCTTCTCTGTGGAGGCTCCGGTCTCTTGTATGCATTGGACAGAAGTGACTGTGG AAAGCAGTGTTTTAACATCGTTTTATAATGCTGAGGATGAGTCCAATCTTCTCTTGCCTAAGCTGCCCAC ATTGCCAAAAAATTACAACAGCACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAATTATCAAGCTC TTGGGAGATGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATG GGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTT GAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAA ACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTC TCTTACAGTATATAAATTTGTCACTGACATGTATGTGTGAAGCATGGGAAGAAATTCTGATGCAGATGGA CTCCCGTCTTACCAAGTTTGTACAGGAAAAACCTACAACCACGTCAGTGCAGGATGAGTTCATGCACTTG CTGCTGTGGGGGAAAGCCAGTGCTGAACTTCAGACTCTCCTGATGAACCAGTTAACAGTGAAGGGCTTAA AAAAGCTTGGCCAGTCTATAGAGTCATCATATTCCAGTATACAAAAACTGGTTATAAGTCACTTACAGAG TGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAA CCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAA ACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGC AATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTT GTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCA ATGTTGAAAGAGTTGGTCAGTATTTGAAAGATGAAGACGACGACCTTGTGTCACCCCCTAACACAGAAGG AAACCAGTGGTATGACTTCCTCCAAAACAGCACCCACCTGAAAGAAAGTCCTTTGCTGTTTCCTTATTAC CCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTTGCAAAAGCCAG CAGATGTAATTGGAAGATCGATGAATCAAGCAATTTGCATTCCATTATATAAAGATGCTAGGAGTATGGA CTGTGCACGAAGATTGTTAAAATTCCCATTTCTGTGGAATAATAAAACTTCGAATCTTCATTATCTTCTT TTTACTATTTTAGAAGATTCGGTTTATAAAATGTGCATCTTAAGGAGACATACTGATATTTCCCAATCTG TAAGTAATGGACTAATTGGTATTAAATTTGGGAGCTTCACATCTGCCTCAGCTGACAAGGTCAGAAGAAG CTCTTACAGTTGTCTAGACGCCCAGTTCTATGATGATGAGACTGTCACGGTCATCCTGAAGGACTCCATG GGACGTGAAGGGAGAGACAGGATTTTGGTTCAGTTGTCACTGTCCTTGGTGTATAACAGTGAAGATTCTG ACGAATATGAGTTCACTGGGAGTTACTCGACAAGGCTTGACGAGCAGGGCAGCATTATCCCCACACGCAC CATGCACTTTGAGAAACACTGGAGGCTGCTAGAAAGCATGAGAGCGCAGTATGTTGCTGGGAACGGCCTT CGAAAAGTGTCCTGTGTGTTAAGTTCAAACCTCCGGCATGTGAGGGTGTTTGAAATGGACATCGATGATG AGTGGGAGATTGACGAGTCTTCAGACGATGAGGAGGAGGCTGGCGGGAAGCCTGTGAAGATCAAGGAGGA AGTGTTGTCAGAGTCAGAGACAGAGGCTCACCAAGACGCTGCTGCCCTAGACCCGGACGTAGTCATCAAA GTGGAGAAACTTGACCCTGAGTTGGACTCGTGACCCAGCTTGACAGCATTGTGTTAGTTAATGTGGCAAA AAGACGTAGTGGACGGACTAAGAATGTATCGGTCACACTGAGTTGTCTGTGTATCAAAGAAATAAATGGT AAATTTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC024870
• Insert Size: 2424 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024870, AAH24870
• RefSeq Size: 2682 bp
• RefSeq ORF: 2424 bp
• Locus ID: 52206
• Cytogenetics: 5 28.81 cM
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]