Copa (BC024070) Mouse Untagged Clone

CAT#: MC206337

Copa (untagged) - Mouse coatomer protein complex subunit alpha (cDNA clone MGC:37774 IMAGE:5097228), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Copa
• Synonyms: AU040324; xenin
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC024070
  CGCTAACCTGACCTGGGAGCCCGAGGCCTGAAGGATGCTAACCAAATTCGAGACGAAGAGCGCGCGGGTC AAAGGCCTTAGTTTTCATCCTAAAAGACCCTGGATCCTGACCAGTTTACACAATGGGGTCATCCAGTTAT GGGACTATCGGATGTGCACCCTAATTGACAAATTTGATGAACATGATGGTCCAGTGCGTGGCATTGACTT CCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGCGATGATTATAAGATTAAGGTTTGGAATTACAAACTT CGGCGCTGCCTGTTCACATTGCTCGGGCACTTGGATTATATCCGTACAACGTTTTTCCATCATGAATATC CTTGGATTCTGAGCGCCTCAGATGATCAGACCATTCGGGTGTGGAACTGGCAGTCTAGAACCTGTGTCTG TGTGTTAACGGGGCACAACCATTATGTAATGTGTGCTCAGTTCCACCCCTCTGAAGACCTGGTGGTATCA GCTAGCCTGGACCAGACTGTCCGCGTTTGGGATATTTCTGGTCTAAGGAAGAAAAACTTGTCTCCTGGTG CGGTGGAGTCGGATGTGAGAGGAATAACTGGCGTTGATCTGTTTGGAACCACAGATGCCGTGGTGAAGCA TGTGCTAGAGGGTCATGACCGTGGAGTCAACTGGGCTGCCTTCCACCCGACTATGCCCCTTATTGTATCT GGGGCAGATGACCGGCAAGTGAAGATCTGGCGTATGAATGAATCAAAGGCATGGGAGGTTGACACCTGCC GGGGCCATTACAACAATGTGTCTTGTGCTGTTTTTCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGA AGACAAGAGTATTCGAGTCTGGGATATGTCTAAGCGGACGGGGGTTCAGACTTTCCGTAGGGATCATGAT CGCTTCTGGGTCCTGGCTGCCCATCCCAACCTTAACCTCTTTGCAGCAGGCCATGATGGTGGGATGATTG TATTTAAGCTGGAACGTGAGCGTCCAGCCTATGCTGTTCATGGTAACATGCTGCATTATGTAAAGGACCG ATTCCTCCGTCAGCTGGATTTCAACAGCTCCAAAGATGTAGCTGTGATGCAGTTGCGGAGCGGTTCTAAG TTTCCAGTATTCAACATGTCATACAATCCAGCAGAAAACGCAGTGCTGCTCTGCACCAGAGCAAGCAATC TAGAGAACAGTACCTACGACCTATACACCATCCCCAAAGACGCAGACTCCCAGAACCCTGACGCCCCTGA AGGGAAACGATCCTCAGGCCTGACAGCGGTTTGGGTTGCTCGGAATCGGTTTGCTGTCCTAGATCGCATG CATTCACTTCTGATTAAAAACCTGAAAAATGAGATCACCAAGAAAATCCAGGTGCCAAACTGTGATGAAA TCTTCTATGCTGGTACAGGCAACCTCCTGCTTCGGGATGCAGATTCCATCACACTCTTTGACGTCCAGCA GAAGCGAACTCTGGCATCAGTAAAGATTTCCAAGGTGAAATATGTTATCTGGTCGGCAGACATGTCTCAT GTAGCGTTACTGGCCAAGCATGCCATTGTGATCTGTAACCGCAAGCTGGATGCTCTGTGTAACATTCATG AGAACATTCGTGTCAAGAGTGGGGCCTGGGATGAAAGTGGGGTGTTTATCTATACCACAAGCAACCACAT CAAATATGCTGTCACCACTGGGGACCATGGGATTATCCGAACTCTGGATTTACCCATCTATGTCACCAGA GTGAAGGGCAATAATGTATACTGCCTAGACAGAGAGTGTCGTCCTCGGGTGCTAACCATTGATCCCACTG AGTTCAAGTTCAAACTGGCCCTGATCAACAGAAAGTATGATGAGGTACTCCACATGGTGAGGAACGCTAA GCTGGTAGGCCAGTCAATCATCGCTTATCTCCAGAAGAAGGGCTATCCTGAAGTGGCGCTGCATTTTGTA AAGGATGAGAAAACTCGTTTCAGCCTGGCCCTGGAGTGTGGAAACATTGAGATTGCTCTAGAGGCAGCCA AAGCCCTGGACGACAAGAACTGCTGGGAGAAGCTAGGAGAAGTGGCCCTGCTACAGGGCAATCACCAAAT TGTAGAGATGTGCTATCAGCGCACCAAAAACTTTGACAAACTTTCCTTCCTGTACCTTATTACTGGCAAC CTGGAGAAACTTCGAAAGATGATGAAGATCGCTGAGATCCGAAAGGACATGAGTGGCCATTATCAGAATG CCCTATACCTAGGTGATGTGTCAGAGAGAGTACGGATCCTGAAGAACTGTGGGCAGAAATCCCTAGCCTA TCTCACAGCTGCTACCCATGGCTTAGATGAAGAAGCCGAGAGCCTGAAGGAAACATTTGACCCTGAGAAG GAGACAATCCCAGACATTGATCCTAATGCCAAGCTGCTCCAGCCACCTGCACCTATCATGCCATTGGATA CAAACTGGCCGTTACTGACCGTGTCCAAAGGGTTCTTTGAAGGCTCCATTGCAAGCAAGGGGAAGGGAGG AGCATTGGCTGCTGACATTGACATCGACACTGTAGGCACTGAAGGCTGGGGAGAGGACGCAGAGCTGCAG CTGGATGAAGATGGGTTTGTGGAGGCTCCAGAAGGTTTGGGAGAGGATGTTCTTGGAAAGGGACAAGAAG AAGGAGGTGGCTGGGATGTAGAAGAAGATTTGGAGCTCCCACCTGAGCTGGATGTACCCTCTGGGGTGTC TGGTAGTGCTGAAGATGGGTTCTTTGTGCCCCCAACCAAGGGAACAAGCCCAACGCAAATCTGGTGCAAT AACTCCCAGCTTCCAGTAGATCACATCTTGGCAGGTTCTTTTGAAACAGCCATGCGGCTTCTCCATGATC AGGTGGGAGTAATCCAGTTTGGCCCCTATAAGCAACTGTTCCTACAGACATATGCCCGAGGCCGCACCAC CTATCAAGCGCTGCCCTGCTTGCCCTCCATGTATAGTTACCCTAATCGCAATTGGAAGGATGCAGGGCTA AAGAATGGAGTACCAGCTGTAGGCCTAAAGCTTAATGACCTGATCCAACGTTTGCAGCTGTGTTATCAGC TCACCACAGTTGGCAAGTTTGAGGAAGCTGTGGAAAAATTCCGTTCCATCCTACTCAGTGTGCCTCTTCT TGTTGTGGACAATAAACAGGAGATTGCAGAGGCCCAGCAGCTCATCACCATTTGCCGTGAATACATTGTG GGTTTGTGCATGGAGATAGAAAGGAAGAAGCTACCTAAGGAGACCCTAGACCAGCAGAAGCGCATCTGTG AGATGGCAGCCTACTTCACACACTCAAATCTGCAGCCTGTGCATATGATCTTGGTGCTGCGGACAGCCCT CAACCTTTTCTTCAAGCTCAAGAACTTCAAGACAGCCGCCACCTTTGCCCGACGCCTGCTGGAACTTGGG CCCAAGCCTGAAGTAGCTCAGCAGACAAGAAAAATCTTATCTGCCTGTGAGAAAAACCCCACAGATGCCT GCCAGCTCAATTATGACATGCACAACCCCTTTGACATTTGTGCTGCATCTTACCGGCCCATCTACCGTGG AAAACCAGTGGAGAAGTGTCCACTCAGTGGGGCCTGCTATTCCCCTGAGTTCAAGGGGCAAATCTGCAGG GTCACTACAGTGACAGAGATTGGCAAAGATGTGATTGGGCTGAGGATCAGTCCTCTTCAGTTTCGCTAAG GCCCTTCCTTCGCGTGCATGGTTAACCCCATACTGTTCTTCAGAGTCTCCCCTTACAGCCACATTCAGCT TGTTGATGTTGGTTCTCCCTAAAACGTCATGTCCTCTCTCTGCTATTGCAAATCAGAAGTTTCAAGCTTG ACTTTCTGGTTCTTGCAGACCTCTGCCTTGACGGTTACTGTGCTTGTGATCATAGATGACTTTGCCTTTC AGCTTAGGCCTTCCAAGGAACAATGAAAACTATGTGCTGGCCATTTGTTTATCAGATTCTTAGTATTTAC TCCTCCCTTCCCACCACCTTCCTGTCAATTCCAGTACGAGACAGAAGGATTCAATAAACGCCACTTTCCC CAGCCACGCGAGTTGTTAACTATCCCATCTTTTTCTTGGGCATTTAATGTCATAAATAGGTTCCTCCTTT ATGTGGCCCCCACACTTCAGGCTGAGAAGAAGCCATTTGGTTTTGCAAAATGTGTACCATTTTTCATTGA TTTGTTTCTATCCTTTATAACATACCATCGTCGTTATGTTCTGTTGCCCTCACTAAATAAAGTAAATTTC TGAGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC024070
• Insert Size: 3675 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024070, AAH24070
• RefSeq Size: 4295 bp
• RefSeq ORF: 3675 bp
• Locus ID: 12847
• Cytogenetics: 1 79.54 cM
• Gene Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function]