BB220380 (BC028998) Mouse Untagged Clone

CAT#: MC206328

BB220380 (untagged) - Mouse expressed sequence BB220380 (cDNA clone MGC:38644 IMAGE:5356085), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: BB220380
• Synonyms: Pik3r6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028998
  TTTTTCTGCAGATCTCTAGAGTGGACAGTCAGGACGACAAGCTCTCTTTGGCTCCTTTGGGACATCTGGT CCTTGGGGTTGCTGGCCTCCTGAACAGGACCTCAGGAGCTCATTGGCTGGAGGGTCTGGGGACGTGGACA ACTGTGGTCCAGCTAAGAAATCTCTGTGTAGAAACCACCATTCTACGTTCTGCTCTGCTGCTTGAGACGA CCACATCCTCCCCCATACAGGACAGACTCCATGGAGAGCTCAGATGTGGAGCTGGACTTCCAGAGGAGTG TACAGGCTGTGCTGCGAGAGCTCAACACCCCAAACCCAGCCCTCCAGAGCAATCAGGGCATGTGGAGATG GTCTCTGCACAAGAAGGTGGAGAGAAACCCAGGAAAGAGCTCCATTCTGGTCCGAATTCTGCTCAGAGAG CTGGAGAAGGCAGAAAGCGAGGACGGACGCCGTGTCATCATTCCCTTGCTGCTCACTCTAATGTCCGTGC TCACTAAGGCCACAGGCATCCCAGAAGATCTGTACCATAGAGCTTATACCTTCTGCACCAGGCTACTGAC CCTGCCGGCCCCCTACTCTACAGTCGCCTTGGACTGCGCCATAAGACTGAAAACAGAGACGGCTGTCCCA GGTACGCTGTACCAGAGGACTGTCATCGCTGAGCAGAATCTGATAAGTGAACTATACCCTTACCAGGAGA GGGTGTTTCTGTTCGTGGACCCTGAGCTGGTTTCTGCCTCCGTGTGCAGTGCCCTGCTGCTGGAGATCCA GGCGGCCCAAGAGCAGCAGACACCGGAGGCCTGCATGCGCCACGTGGTCTCCCACGCCTTGCAGGCAGCT CTGGGAGAAGCCTGCCACACAGGTGCTCTGAACAGGAAGCTGCAGGCCAGCTCCCGACGCGTCCTGGAAT ACTATTTCCACGCCGTTGTGGCTGCCATAGAGCAGGTGGCTAGCGAGGACAGCCCCAGCCGGCTGGGACA CCTGGAGAAGATGGAGGAGATTTACTGCTCGCTGCTAGGGCCTGCGACCACAAGAAGGCACTGTGTCGGT GACCTTCTGCAAGACCGCCTGCCAAGCATCCCCCTGCCCAGCCCCTACATCACCTTCCACCTCTGGACCG ACCAGGAGCAGCTCTGGAAGGAACTGGTACTCTTCCTCCGCCCACGCTCGCAGCTGCGCCTCAGTGCTGA TCTGGATGCCTTGGATCTACAAGGATTTAGGCTAGACCGGGACTTGGCCCGGGTGTCCACCGACAGTGGA ATTGAGCGGGACCTCCCTTTGGGGAGCGATGAGCTTCCGGACCCCAGCAGCTCTGAGATGGAGCGGGCCG CCCTTCAACGCAAGGGGGGCATCAAGAAGCGTGTGTGGCCCCCTGACTTCTTCATGCCTGGCAGCTGGGA TGGGCCCCCGGGGTTGCATCGGAGGACTGGCAGGCCTAGTGGGGACGGGGAGCTGCTACCTGGAGTCTCT CGGGTGCATACCGCCCGCGTGCTCGTTCTGGGGGATGACAGGATGCTAGGGCGCTTGGCCCAGGCTTACT ACAGACTCAGGAAACGGGAGACCAAGAAGTTCTGCCTCACTCCTAGACTCAGCCTACAGCTCTACTATAT CCCTGTGCTGGCGCCTCAGGATCCAGAAGCATCCAGGAAGCCTGAGCTGGGAGAGCTGGCTTCCTTCCTG GGTCGAGTCGACCCATGGTATGAAAGCACTGTCAACACCCTGTGTCCTGCCATCCTCAAGCTGGCCGAGA TGCCTCCCTACTTGGACACATCTAGGACCGTGGACCCCTTCATCCTGGATGTCATCACTTACTATGTCCG CATGGGCACTCAGCCCATCTATTTCCAGCTCTACAAGGTTAAGATCTTCACCAGCTTGAGTCACGACCCT ACAGAGGACATTTTCCTCACAGAGCTGAAGGTGAAGATCCAAGACTCCAAGTCCCCCAAAGAGGGCTCTT CACCTCGAAGGAGAGGGGCAGCTGAGGGCACAGGAGCTGAGCTGTCCATGTGCTACCAGAAGGCCCTGCT CAGCCACAGACCACGAGAGGTCACTGTTTCTCTGCGGGCCACCGGGCTGGTCCTGAAGGCCATTCCAGCT GGTGACACTGAAGTTTCAGGGTTCTTCCACTGTACCTCACCAAATGCTGCTTCTGCCACAGACTGTTCCT GTCTGCACGTCAGTGTGACAGAGGTGGTCAAGTCCTCCAATCTGGCAGGAAGGTCATTTACGACATCAAC CAACACCTTCCGGACATCCAGTATCCAAGTCCAGAGCCAAGACCAGAGGCTGCTCACACTATGGCTGGAC AAGGACGGACGGCGGACTTTCAGGGATGTGGTCAGATTTGAGGTCAGTCCCTGCCCAGAGCCATGTTCAC GGACCCAGAAATCCAAGACGTCAGCGCTCAATTCGCACGGGCAGGAGACAGAGAAGAACATGGCCAAGCC CAATTCTCTTCTGATGCCCATCAACACATTCTCTGGCATCATCCAATAAGCTTACCAGGACAGTGGGAGA TCTGGGTCCAGGGGCAGCAGACCCAAGAAGGAAGAAACACTACCCACCCATCCACAACATTTACACTGAC AGCCCCACTGGGCAGCTCTGCTTGGACCAGGAGCCAGAGAATGCCTGGGAATTAAAAGAGCCTGGGTTGT AATGAATAAGGGGCATGTGATAAAGATGGATGCAGCCCACGGCTCCCAGATTGCTGGACAGAGTTGGAAT GAGATGAGCTGATGCATACTCAAGCCAGCTTCTCCCAGAAACATCTTTCTTGTGATGATGTTCATTTCCC GTTTCTCATCCACCATCCACTCCAGCTCCCTAGGGAGTCCTGAGAACTGGTGTCAGTTAGCTGCAGGTAT AGCTGGTGGTTCAGCGGTAGAGCACTTGCCTAGTGTGTGGGAGGCCTTGGGTTTGACCTCAGTTTATAAA AAGGGGTTCAGAGCTGATGGGTTCACTATTTGTACAGCGCCCTGTGGCTTTGGAAGGACCAGGGAGTAGG TAGTGAATACTGTATTTGCTCCCTTCTGACTTCATACCATCCTTTGCAGCTCTCAAGGCCACGGTTCGCC AGTGCCGAGGGGGGTCGGTCTGTCTCAGTAAAGAAGAGAGTGATAGTCTCAATAAAAGCACACTAAAGTG TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC028998
• Insert Size: 2259 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028998, AAH28998
• RefSeq Size: 3200 bp
• RefSeq ORF: 2259 bp
• Locus ID: 104709
• Cytogenetics: 11 B3
• Gene Summary: Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 11. The protein binds to both the catalytic subunit and to G beta-gamma, and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]