Gtf2e2 (BC020016) Mouse Untagged Clone

CAT#: MC206311

Gtf2e2 (untagged) - Mouse general transcription factor II E, polypeptide 2 (beta subunit) (cDNA clone MGC:29023 IMAGE:3501113), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2e2
• Synonyms: 34kDa; AI462509; C330006J08Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020016
  CCGAGGCGGAGGCTGGGAGGCGGCGCGGGCTGCGGGCGCTGAGCATTATGGATCCAAGCCTATTGAGAGA TCGAGAGCTGTTCAAAAAACGAGCTCTTTCCACTCCTGTGGTAGAAAAACGTGCTGTGCCTTCTGAGTCA CCCTCCTCCTCCTCATCCAAGAAGAAGAAGGCAAAAGTCGAACATGGAGGATCCTCAGGCTCTAAACAAA ATTCAGATCATAACAATGGATCATTTAACTTGAAAGCTCTGTCTGGAAGCTCTGGATATAAGTTCGGTGT TCTTGCTAAGATTGTGAATTATATGAAAACACGACATCAGCGGGGAGATACACATCCTCTAACGTTGGAA GAGATTCTGGATGAGACACAGCACTTAGACATCGGACTCAAGCAGAAACAGTGGCTGATGACGGAGGCTT TAGTCAACAATCCTAAAATTGAAGTAGTGGATGGGAAATATGCCTTCAAACCTAAGTACAACTTGAAAGA TAAGAAGGCCCTGCTTAGGCTTTTAGACAATCATGACCAGCGGGGACTAGGAGGAATTCTCTTAGAAGAC ATAGAAGAGGGACTGCCTAATTCCCAGAAAGCTGTCAAGGCTTTAGGGGACCAGATACTATTTGTAAGTC GTCCTGATAAGAAGAAAATACTATTCTTCAATGATAAAAGCTGTCAGTTTTCTGTGGATGAAGAATTCCA GAAACTGTGGAGGAGTGTCACTGTGGATTCAATGGATGAGGAGAAAATTGAAGAGTATCTGAAGCGACAG GGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCTCTATTCAGAGAAGGAAAAAGCCTGCTT CACAGAAAAAGCGACGGTTTAAGACTCACAATGAACACTTGGCTGGAGTGCTGAAGGATTACTCGGACAT TACCCCGGGCAAATAGGGAACACTTTTATTCCTAAACACAGTTACAGACACGCAGTCGAGATCTTTCTTG ATGCTTGGGATCTCAAGACTGTCTGCCTTCCTGCCTGCTCCTCAGGCCTGAGAGAGGAGCTGCTCAGTTT ACAGAACAGGAGCGGGTTACCAGACTCATTCTTTTCATCCAGCAGGCTAGTGGGAAGGAATGGGTGTTCC TGTTTAACTTTCTGGGTTTGTTTGTTTTATTTTATTAGAAAGACACTTATATGACACTCAGGTGTCTGCT ATTTATCTTGATTTCTAAATGGTTCTGGATTCCATGTTTTCTCTATTCGTTTAGAAGTTGGAGGATGGTG CTGAATGCAGTTTTGTTTATTCCAAGAAAAATATTTATAATAAAATCATTTGTAGGAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC020016
• Insert Size: 879 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020016, AAH20016
• RefSeq Size: 1415 bp
• RefSeq ORF: 879 bp
• Locus ID: 68153
• Cytogenetics: 8 A4
• Gene Summary: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase (By similarity).[UniProtKB/Swiss-Prot Function]