Rtcd1 (BC016519) Mouse Untagged Clone

CAT#: MC206308

Rtcd1 (untagged) - Mouse RNA terminal phosphate cyclase domain 1 (cDNA clone MGC:27879 IMAGE:3495638), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rtcd1
• Synonyms: 2310009A18Rik; AI450277; RPC; Rtcd1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016519
  GCGAGCTCGGCCCGGAAGCGCACTGTCAGGCCTGCGGGCTTGCCGGCGCCTCGTCTTTTGCCGGTTGCAG CAGGGACGTGGTCGCTGCCGGCCGGGCTCAGAGGCGCCCGCCATGGAGGGCCAGCGGGTGGAGGTGGACG GTGGGATCATGGAAGGGGGCGGCCAGATCCTCAGGGTCTCCACCGCCCTGAGCTGCCTCCTGGGCCTACC CTTGCGCGTGCAGAAGATCCGCGCGGGCCGCAGCACGCCGGGCCTCAGGCCTCAGCATTTATCTGGACTG GAAATGGTTCGTGATTTGTGTGGCGGGCACCTGGAGGGAGCAGAAATCGGCTCAACAGAGATAACCTTTA CCCCCGAGAAGATCAGAGGTGGAGTCCACACGGCAGATACCAAGACAGCAGGGAGTGTGTGCCTCTTGAT GCAGGTTTCAATGCCCTGTGTCCTGTTTGCGGCTTCCCCGTCGGAGCTCCGTCTGAAAGGCGGCACCAAT GCGGAGATGGCCCCGCAGATCGATTACACCATGATGGTTTTCAAGCCAATTGCTGAGAAGTTTGGTTTCA CATTTAATTGTGACATTAAAACGAGGGGCTATTACCCCAAAGGGGGTGGTGAAGTGATTGTCCGAGTGTC CCCAGTTAAACGATTGGACCCAATAAATCTGACTGATCGAGGCTCTGTGACCAAGATTTATGGGAGAGCT TTTGTTGCCGGTGTTTTGCCACTAAAAGTAGCAAAAGATATGGCGGCGGCGGCTGTGCGGTGCATCCGAA AGGAGATCCGGGATCTGTACGTCAGCATCCAGCCCGTGCAGGAGGCCAGGGACCAAGCCTTCGGCAATGG GAGTGGAATCATCATTGTTGCTGAGACATCCACTGGCTGCTTGTTTGCTGGATCATCACTTGGCAAAAGA GGTGTGAATGCAGACAAGGTTGGGATTGAAGCTGCTGAAATGCTATTAGCGAACCTTAGACACGGTGGCA CTGTGGATGAGTATCTACAAGACCAGCTGATTATTTTCATGGCGCTAGCCAATGGAATTTCCAGAATAAA AACAGGGCCAGTCACACTCCACACACAGACTGCTATACATTTTGCTGAACAGCTAGCAAAGGCCAAATTT ACCGTGAAGAAATCAGAAGAGGAAGAGGATGCCACTAAGGACACTTACATCATCGAGTGTGAAGGAATTG GGATGGCAAATCCACACCTCTAGGGTGTTCACGTACGCTCTACCTCCTTGTTGCACTGACTTAGCCCATG CATGCTGCAACACAGTGAGTGAGACGTCAGCTACCAAAGGACTTGACTCTTTGGAGATGGAGGTTGGCAC TGTCAGTTGCTCAGAATGCTCCATCATGTTAAGCTTGTGGGATATCTCCCAAGATACAGACAGTGAACGA TCAAACGTAATGAATATGTGTAATAGTCAACTTCAATATTAACATCATACCCTAACGTTATAAATAAATT GTTGTTCATACCCTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC016519
• Insert Size: 1101 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016519, AAH16519
• RefSeq Size: 1499 bp
• RefSeq ORF: 1101 bp
• Locus ID: 66368
• Cytogenetics: 3 G1
• Gene Summary: Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).[UniProtKB/Swiss-Prot Function]