Pi4k2a (BC022127) Mouse Untagged Clone

CAT#: MC206278

Pi4k2a (untagged) - Mouse phosphatidylinositol 4-kinase type 2 alpha (cDNA clone MGC:37783 IMAGE:5097465), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pi4k2a
• Synonyms: MGC37783, Pi4k2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC022127, the custom clone sequence may differ by one or more nucleotides
  
  
  CCCACGCGTCCGCGGACGCGTGGGCCGGCGGCCTGAGGGATGGACGAGACGAGCCCGCTAGTGTCCCCCG
  AGCGGGCCCAACCCCCGGAGTACACCTTCCCGTCGGGCTCCGGAGCTCACTTTCCGCAAGTACCGGGGGG
  CGCGGTCCGCGTGGCGGCGGCGGCCGGCTCCGGCCCGTCACCGCCGTGCTCGCCCGGCCACGACCGGGAG
  CGGCAGCCCCTGCTGGACCGGGCCCGGGGCGCGGCGGCGCAGGGCCAGACCCACACGGTGGCGGTGCAGG
  CCCAGGCCCTGGCCGCCCAGGCGGCCGTGGCGGCGCACGCCGTTCAGACCCACCGCGAGCGGAACGACTT
  CCCGGAGGACCCCGAGTTCGAGGTGGTGGTGCGGCAGGCCGAGGTTGCCATCGAGTGCAGCATCTATCCC
  GAGCGCATCTACCAGGGCTCCAGTGGAAGCTACTTCGTCAAGGACTCTCAGGGGAGAATCGTTGCTGTCT
  TCAAACCCAAGAATGAAGAGCCATATGGGCACCTTAACCCTAAGTGGACCAAGTGGCTGCAGAAGCTATG
  CTGCCCCTGCTGCTTCGGCCGAGACTGCCTTGTTCTCAACCAGGGCTATCTCTCAGAGGCAGGGGCTAGC
  CTGGTGGACCAAAAACTGGAACTCAACATTGTACCACGTACAAAGGTAGTATACCTGGCCAGTGAAACCT
  TCAACTACAGTGCCATTGACCGAGTAAAGTCCAGGGGCAAGCGGCTTGCACTAGAGAAAGTGCCAAAAGT
  TGGGCAGCGGTTTAACCGAATCGGCCTGCCACCAAAGGTCGGGTCATTCCAGCTTTTCGTTGAAGGCTAC
  AAAGATGCAGACTATTGGCTGCGGCGTTTTGAAGCAGAACCTCTCCCTGAGAACACGAACCGACAGCTGC
  TATTGCAGTTTGAGCGGTTGGTGGTCCTGGACTACATCATCCGCAACACTGACCGAGGCAATGACAACTG
  GTTGATCAAATATGACTGTCCGATGGATAATTCTAGCTGTCGGGACACAGATTGGGTGATGGTGAGGGAG
  CCTGTTATCAAGGTGGCTGCCATAGACAACGGGCTAGCTTTCCCACTGAAGCATCCTGACTCCTGGAGGG
  CATATCCTTTTTACTGGGCCTGGCTGCCTCAGGCGAAAGTCCCGTTCTCTCAGGAGATCAAAGATTTGAT
  TCTTCCAAAGATTTCAGACCCTAACTTCATCAAGGACTTGGAGGAGGACCTATATGAACTCTTCAAGAGA
  GATCCTGGCTTCGACAGGGGCCAGTTCCATAAGCAGATTGCTGTCATGAGAGGCCAGATCCTAAATTTGA
  CCCAGGCCCTGAAAGACAATAAGAGCCCCCTGCACCTCGTCCAGATGCCACCTGTGATTGTCGAGACGGC
  CCGCTCTCATCAGCGGTCTGCAAGCGAATCCTACACACAGAGCTTTCAGAGTCGGAAGCCCTTCTTTTCA
  TGGTGGTAGCCCCAGAGGCAGGCAGAAGAAATCCTGCAGGAGCCAGCAGGGGTCCAGGTGTGTGCCGGTC
  TTCACAGATGTTTCCACACCAGCCGGGTGGCGAGACTTCCCCCTGGAGTGCTGGAGAGAACTTAGTCCTG
  CCCCAGACCAGGTTTGCCATCAGGAAGAAATTGGGTCCAATGGAAGAGGGGGCCGGTGCCAGGCCCAGTG
  GGCAAGGGTGCAGGCAGCCTTACCTGCCTCCCTCCCTCGAAGCTTGTACTCAAGTTGCCTTGAATGTGGA
  CTTGGGAGTGCCAGGAGCCCAGAGTGTCAGGTACTTCAGAACAGCCGCCCCTCTGCCAGCCTTAGGCCTG
  TCCCCTGTGTCTAGGAGCTGAAGCTGGCTGGTGAGCGCTGCTGCCCTGTTCTTCTAATGCACTGTAGAAA
  TTGTATGTATTGTATTTAAATCAATGCAAATGTATGAATAACAAATCCAGTTCTGAAAAAAAAAAAAAAA
  
  
• Restriction Sites: EcoRI-NotI
• ACCN: BC022127
• Insert Size: 1482 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022127
• RefSeq Size: 1960 bp
• RefSeq ORF: 1482 bp
• Locus ID: 84095
• Cytogenetics: 19 35.74 cM
• Gene Summary: Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).[UniProtKB/Swiss-Prot Function]