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Nkd2 (BC019952) Mouse Untagged Clone

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SKU
MC206269
Nkd2 (untagged) - Mouse naked cuticle 2 homolog (Drosophila) (cDNA clone MGC:28383 IMAGE:4021772), (10ug)
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  $457.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nkd2
Synonyms 2210403L10Rik; AW212591
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC019952
GCCGCTGCCCGCGCCGGTGTCTCCGGTTGCCGGGCCAAGCCTCGCCAGACTGCCCGGCCCCGGATCCAGG CTCCACGAGTTGCTCTCAGCTCGGGATTCACGCACCCCGGGCTCGAACCTCGGACGCACACCTGAGAGCT CGGCCGGAGGGTGGGTCCAGGCCCAGCGGAGACATGGGGAAATTTCAGTCCAAGCACGCCGCAGCAGCCT GCAAGAGGAGAGAGAGTCCCGAAGGGGACAGCTTCGTGGTGCCTGCGTACGGGAGCGGTCGCAGAGGAGC GGAAGAAACGGACCGGCGTGCGGGCAGCGGTGTGGAACATCGCTCACGGGACAAGCAGGAGCTGCTCAAT GGAGACCCTAAGGAGGGGCCTTTCTGGGACGACAAGGGTTCCCTAGAAGTTGCGCTGCCCCCTGAGAAGT CTGAGGGCCATGAGGGCCAGGGCCAGCTCTTCAGCACAGATGATGGGGAGAAGGCAGCAAGCCGTGAGGG TCCACTGAGACTTAGCAAGAAGCACTTGAACATTGACGCACTGCAGTGTGACGTCTCAGTGGAAGAAGAC AACCGCCAAGAATGGACATTCACGTTGTATGACTTTGACAACAGTGGGAAAGTCACCAGAGAGGATATGT CCAGCCTGATGCACACCATCTACGAGGTTGTCGATGCCTCTGTCAATCACTCCTCCGGCAGCAGCAAGAC CCTTCGAGTGAAGCTAACTGTCAGCCCTGAACCCTCCAGCAAGAAGGAATGTCCTCTCACTGGCCAAGAC CGGGAGCCCACTCGTGGCAGAACAGAGATTGAGCTCACAGATGAGCCCCGAGCGGCTGACAGAAGGCTAT CCGCCTACAGCAGGAAGCCCAATGCTGATCCCCAGCCCTGCTCTGTGCGAGTGCCCTACTGTGTGGATGA GAACACAGAGCGCAGAAACCACTACCTAGACCTTGCTGGCATCGAGAACTACACATCTAAGTTTGGTCCT GGGTCACCACCTGAGCAGGCCAGGCAAGAACATCATGGCAGGGCCACACACATTCCAAGCAGGTCCCGAT CACAAGAGTCGGATGCCCACGCTATACACCACCGCAGGTCTCAAGTCCTGGCTGAGCATGTCATACCAGC TAATGAGCCTGCCACCCGGGCCCTGGCTGCACAGCCCCGGCTCAAGGGGCAGGAGAAGCAGTTCCTCAGG TCTCCTAAGGGTCCAGGAAAACCTCTTGGGACACCAGGCAGTGGCAAGCCAGGGAAAGCTCTCAGCTATT GCCTGCAGGCTGTGCCACTGCCCCAGGGTGCTCAGGATGGCCACCACCTTCCTCAGCCCCCACCACAGCC TCCACCGCAGCCCTATGGTCACAAGCGGTACCGGCAGAAAGCCAGAGAAGGCCACTCACCACTTAAGGGG CATGGCCAGCCTACCATGGTGGAGCATGAAGTGGTACGGGACCTGCCTCCCATGCTGGGGCCTGAGGGCT ATGTGATGCCTGTGGTCCAGAGGCATGAACACCACCATCACCATGAGCACCACCACCATCACCACCACCA CCACTTCCACCCATCCTAGCACCCAAACAAGCACTCTGCCCAAATGCCCTTGGGAACAGAGCAGGGGCTG GCATGTGCCTAGTAGAGAATACAGCATCCCCTGGCTCAACACCAGCTACAGATGTCAAGGGGGCACACCA GGGAGATGGCACCAGGCTTGGATATTCATGGACAAAGCTACCTTTCTCCATGCCATTTGGATACTAGTAC ATTTGATGCAGCTGATGGTTACCAGGGACACGTGCTCCTGTGTCATCCAGAAAGCCCATTCATGAGGGCA CAAAAGCATCTGTTCCTGCTTTTGCTGCAGTGTGGCCATGGGATATTTGAAGGCATTCTTTGTATCCTCA TGGTTCTTTGACCTTCGGGTACCTGAATAGAAAAGGCAATGCTTTTTGTGAAAAAAAAAAAAAAAAAAAA AAAAAAA
Restriction Sites EcoRI-NotI |
ACCN BC019952
Insert Size 1386 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019952, AAH19952
RefSeq Size 1967 bp
RefSeq ORF 1386 bp
Locus ID 72293
Cytogenetics 13 C1
Summary Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells (By similarity).UniProtKB/Swiss-Prot Function
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