Nsf (BC006627) Mouse Untagged Clone

CAT#: MC206238

Nsf (untagged) - Mouse N-ethylmaleimide sensitive fusion protein (cDNA clone MGC:11511 IMAGE:3968733), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsf
• Synonyms: AI316878; AU020090; AU067812; SKD2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006627
  CGGACGCGTGGGCGCGCGCGGCGGAGGCGGGCTCAGCAGAGTGCGGAGCTGCAACCTGGGAGCCGGACGT GTCCGCGAAGATGGCGGGCCGGACTATGCAAGCTGCGAGATGCCCTACTGATGAATTATCTTTAAGCAAC TGTGCGGTTGTGAACGAAAAAGATTTCCAGTCTGGCCAGCATGTGATGGTGAGGACGTCCCCCAATCACA AGTACATATTTACGCTGAGGACCCATCCGTCAGTGGTTCCAGGATGCATCGCGTTCAGCTTGCCTCAGCG AAAATGGGCTGGACTTTCTATTGGACAGGACATAGAAGTTGCCTTGTATTCGTTTGACAAAGCCAAGCAG TGCATCGGCACAATGACCATCGAGATCGACTTCCTGCAGAAGAAGAACATCGACTCCAACCCTTACGACA CCGACAAGATGGCCGCCGAGTTCATCCAGCAGTTCAACAACCAGGCCTTCTCAGTGGGGCAGCAGCTTGT CTTTAGCTTCAATGATAAGCTCTTTGGATTACTGGTGAAGGACATTGAAGCCATGGATCCCAGCATCCTG AAGGGAGAGCCTGCATCAGGCAAAAGGCAGAAGATTGAGGTAGGCCTGGTTGTTGGAAACAGTCAAGTTG CATTTGAAAAAGCAGAAAATTCGTCGCTCAATCTTATCGGCAAAGCTAAAACCAAGGAAAATCGCCAGTC CATCATCAATCCCGACTGGAACTTTGAGAAAATGGGGATCGGCGGTCTGGATAAGGAGTTCTCGGACATT TTCCGAAGAGCATTTGCTTCTCGGGTGTTCCCCCCGGAGATCGTGGAACAGATGGGTTGCAAACATGTTA AAGGCATCCTGTTATACGGACCCCCTGGTTGTGGTAAAACTCTCTTGGCTCGACAGATTGGCAAGATGCT GAATGCGAGAGAGCCCAAGGTGGTCAATGGGCCAGAAATCCTTAACAAGTATGTGGGAGAATCAGAGGCT AACATTCGTAAACTCTTTGCTGATGCCGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGTGGCTTGCACA TCATCATCTTTGATGAAATCGATGCCATCTGCAAGCAGAGAGGGAGCATGGCCGGCAGCACTGGAGTGCA TGACACCGTGGTCAACCAGCTGCTGTCCAAGATCGATGGCGTTGAGCAGCTGAACAACATCCTCGTTATC GGAATGACCAATAGACCAGATTTGATAGACGAAGCTCTCCTTCGACCCGGAAGACTGGAAGTTAAAATGG AGATAGGCTTGCCAGATGAGAAGGGTCGACTCCAGATCCTTCATATCCACACAGCAAGGATGAGAGGGCA CCAGTTACTGTCTGCAGATGTGGACATCAAGGAGCTGGCTGTGGAGACTAAGAATTTCAGTGGCGCTGAG CTGGAGGGTCTGGTGCGAGCAGCGCAGTCCACAGCCATGAACAGACATATCAAGGCCAGCACCAAAGTAG AAGTAGACATGGAGAAGGCAGAGAGCCTGCAGGTGACAAGAGGGGACTTCCTTGCTTCCCTGGAGAACGA TATCAAACCAGCATTTGGCACCAACCAAGAGGATTATGCAAGTTACATTATGAATGGGATCATCAAGTGG GGTGACCCCGTGACCCGAGTCCTAGATGACGGAGAGCTGCTGGTCCAGCAAACGAAAAACAGTGACCGGA CACCACTCGTTAGTGTCCTCCTAGAAGGGCCTCCTCACAGCGGGAAGACTGCCCTGGCTGCAAAGATTGC CGAGGAATCCAACTTCCCCTTCATCAAGATCTGCTCACCTGATAAGATGATTGGCTTCTCCGAGACAGCC AAGTGTCAGGCCATGAAGAAGATCTTTGATGATGCTTACAAATCTCAGCTCAGCTGTGTGGTCGTGGATG ACATTGAGAGGCTGCTCGATTATGTCCCCATTGGTCCCCGGTTCTCCAACCTGGTATTGCAGGCTCTTCT TGTGTTACTGAAAAAGGCGCCTCCTCAGGGCCGCAAGCTTCTTATCATCGGGACCACCAGCCGCAAAGAT GTCCTGCAGGAAATGGAGATGCTGAACGCCTTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAG AGCAGCTGCTGGAAGCTCTGGAGCTTTTGGGCAACTTCAAAGATAAGGAACGCACCACAATTGCTCAGCA AGTCAAAGGGAAGAAAGTCTGGATAGGAATCAAGAAGTTACTAATGTTGATTGAGATGTCCCTGCAGATG GATCCTGAGTACCGTGTGAGGAAATTCTTGGCCCTCATGAGAGAAGAAGGCGCTAGTCCCCTGGACTTTG ATTGAAAACGGACTGTTTACAACAGTGACCAAGGGAAGCGACCAAGGTGCAGATGGCCTGGGATCGTCAC TCCTCACACTCAAGACACTGGACTACGTGAAGTGTCCGCCTCTCTCCACACACTCGCTCTGCATGACTAG TGCAGTGAACTCCCTCGTGCTTTTGAGATGTTCTCGTGTTCCCTGGACTGCTGGGCTTCCCTCCCGTGCT AGCTAAGATGGTTCCTGCTTATGTGGGAGGCATGTCACACCCGTGCACGCTGCCCACACCGCGATCCCTA CCACCTGCCCGAAGGTCAACTTCTACATGAGACCAAGAGCAAAGGTAGAGGTTCCAAGGGAGTCCCTTGG CGCTCCCCATGCTAACAGCTGTGTATCCCGCAGATTAGGTCATTGTCTGTTCCTAAAGAACTAGGCTTTG AATATCTAAGATGCTGTTCTCAATCATTTTCAGAGATATTTCCAAGTTGCAATCAGGAGAGCTTTTCTAA TCGGAAGTCAAATCATTCCTGGGGTGGCTGTGATATGCTCTGAGTGTAATTAATTTGCTCTGTGGTAAGA GATATGCTAATCATTGCCACTTGGTAGATGCCGTTAAAACCTTATGAAAGATACATATGGAAATCTGTAC ATCCCTAAACAGAAAAAAAATTAAAATAATTTACAAGCCTCTCAATTCTAATTAGCGTCCGGCTCTAAGT GGGTCAGTTCCTTAGGATGACGTTAGAGGCCTATTAGTGCCATCGTCTTCCTTTTCCTCAGCCTGATGAC TTACAATTCATCCTCCTTGTATCTCAAGTGATCTGTAGTGTCTGCAGCACATAAATAAAACACTACTCTC CAGCAGAACAAGCCACCCCCTAGTCCTCTCCCTCAGCCCTCGAATATCCATTCCAGCGTACTCAACACAC CTCTTCCCGAAGAGGAACTCAGCGAGCCCTTTGGAGGGTGGTTTGCCTAATCAGTGGGTTAGAAGGAATG CAATTAGGTCGTCACGTCATTCGTGGAGATGAGTCTTAGGGCGCATGCCACTCTCCCCGCTGCTGCTTCA AACACAAAGACAGCCATCAGAAGCCGCCCGGGAGGCTTGCTGCCGCAGGGGCACCTGGGTGATACTCTGA CACTGTCCCCACAGTAGAAGGCAAATCATCTCCTAGTCTTGCCGTCTGTATACCTTGTTTGGGGTTTTCA TCCCTGGCTTATTTTGTGGCTTTGAAAGAGTGAATTGTGTTGTGAAACTGTGATTCCCGTGGCTAGTACC GTGGTTCCCTGTCCACCACCGTGGTCCCTGTTGTCTGCAGTTCTGCCTTTCTCCTCCCAACAAAGTCCTA CATGAATTAATTTATGTTTCTTGTTCAACGAAGTTAATGTCTTGTGTTTTTTGTCCAAAATTATATTGAA AAAGATATTATATTGTGTAATGCAAATTGAAGGAATGCAATAAAGAGTAAATATACTTGAAAAAAAAAAA AAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC006627
• Insert Size: 2235 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006627, AAH06627
• RefSeq Size: 3787 bp
• RefSeq ORF: 2235 bp
• Locus ID: 18195
• Cytogenetics: 11 67.54 cM
• Gene Summary: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).[UniProtKB/Swiss-Prot Function]