Txnrd3 (BC076605) Mouse Untagged Clone

CAT#: MC206218

Txnrd3 (untagged) - Mouse thioredoxin reductase 3 (cDNA clone MGC:100108 IMAGE:30628949), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Txnrd3
• Synonyms: Tgr, TR2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC076605
  CGTTCTCAAGTTCTCAGCGGCTTTGCCGGCGTCGTCCCCTAGGCGGCCGCCCGCGTCCCGGTTCCTCAGC AGGCCCGGCTCGGCCCGGAGCGACAACAAAGCCCTGGAGAAGCCACCGTCCCCGCCGCCGCCTCCTCGGG CGCAGACCTCGCCCGGGCTGGGGAAGGTTGGAGTCCTCCCCAACCGCCGCCTGGGTGCTGTCCGTGGCGG CCTCATGTCGTCGCCACCCGGCCGCCGCGCCCGCCTGGCCTCCCCTGGGACCAGCCGCCCGTCTTCTGAG GCCCGCGAGGAGCTGCGGCGCCGCCTGCGGGACCTCATCGAGGGCAACAGGGTGATGATCTTCAGCAAGA GTTACTGTCCACACAGCACGCGGGTTAAGGAACTCTTTTCGTCCTTGGGAGTGGTCTATAACATCCTGGA ACTTGATCAAGTTGATGACGGGGCCAGTGTTCAGGAAGTGCTGACAGAAATCAGTAACCAGAAAACGGTG CCCAATATTTTTGTGAATAAAGTGCACGTGGGTGGATGTGACCGAACTTTCCAGGCACATCAGAATGGTT TACTGCAGAAGCTCCTCCAAGATGACTCGGCTCATGATTACGACCTCATCATCATCGGCGGGGGTTCTGG CGGCCTCTCTTGTGCCAAGGAAGCTGCCAACTTGGGAAAGAAGGTCATGGTGCTAGACTTTGTGGTCCCA TCGCCTCAGGGCACGACCTGGGGCCTTGGCGGCACCTGTGTGAACGTAGGCTGTATTCCAAAGAAGCTGA TGCATCAGGCAGCCCTCCTGGGGCATGCTTTGCAAGATGCCAAGAAATATGGCTGGGAGTATAACCAGCA GGTGAAGCACAACTGGGAGGCCATGACAGAAGCTATCCAGAGCCACATTGGCTCCTTGAACTGGGGCTAC AGGGTAACCCTTCGGGAGAAAGGCGTGACCTATGTCAACTCCTTCGGGGAGTTTGTGGACCTGCATAAAA TAAAGGCAACCAATAAGAAAGGGCAGGAAACGTTTTACACGGCTTCGAAGTTTGTCATAGCAACAGGTGA AAGGCCTCGGTACTTGGGAATCCAGGGAGATAAAGAGTACTGTATTACGAGTGACGACCTGTTCTCTCTG CCATACTGCCCGGGATGTACATTAGTCGTAGGCGCTTCTTATGTTGGTCTGGAGTGTGCGGGCTTTTTGG CTGGTTTGGGGTTAGATGTGACAGTCATGGTACGCTCCGTCCTTCTTCGTGGCTTTGATCAAGAAATGGC AGAGAAAGTGGGATCCTACCTGGAACAACAAGGTGTCAAGTTCCAAAGGAAATTCACCCCCATTTTGGTT CAACAGTTGGAGAAAGGTTTACCAGGAAAATTGAAAGTCGTGGCTAAGTCCACCGAAGGACCGGAAACAG TAGAAGGGATATACAACACGGTTTTGTTAGCAATTGGTCGTGACTCCTGTACAAGGAAAATAGGGCTGGA GAAGATCGGGGTCAAAATCAATGAGAAGAATGGCAAAATACCAGTAAACGATGTGGAGCAGACCAACGTG CCTCATGTTTATGCTATTGGGGACATACTGGACGGCAAACCAGAGCTCACCCCCGTTGCCATACAGGCAG GCAAGCTGCTAGCTCGAAGACTCTTTGGGGTCTCTTTAGAAAAGTGTGATTATATTAACATCCCAACAAC GGTGTTCACACCTCTGGAATATGGCTGTTGTGGACTGTCGGAAGAGAAAGCCATCGAAATGTATAAAAAA GAGAATCTGGAAGTGTATCACACCTTGTTTTGGCCTCTCGAGTGGACAGTTGCTGGCAGAGACAACAACA CCTGTTATGCAAAGATAATCTGCAACAAATTCGACAACGAACGTGTGGTGGGATTTCACCTTCTGGGGCC AAATGCTGGTGAAATCACGCAGGGATTTGCAGCTGCAATGAAATGTGGGCTCACGAAGCAGCTACTGGAT GATACCATTGGAATCCACCCCACCTGTGGTGAGGTATTCACAACATTGGAAATCACAAAGTCCTCAGGGC TGGACATTACTCAGAAAGGCTGCTGAGGCTAGCCCTGCTGCTGTTGGGTTTCCCTGTCACACTCTCACTC TGTGCCCATGACATCTGCTTGGGCACTGGGGACCAGTGCCGGACTTTCTCCTGGCATAGAGACGAGAAGG CGGCAGAGGACCAGCAGCAGCAGCAGCATCTGGAGTCTTAGCTGACGGCATGCAGCAGCCAGGCTGCTTC CTTGACACCTTGGCTCGGAACCTGCAGAGGTGAGCCAAGGCCGACTTCTGCACGTCAGCCTCGACTTCAC CCCACGAGGCCTTAGATGGCACAGTGAGCCCACCTCCCTTTCCCAGTTGCTAGTGCTTTGACCCCTCACT TTGTTTCCATGAAGAGATTATCTTTCAGTTGTGGACTTGACTGTAAAGAGGCTTGTTGCCCAGAGTGTAG CACTTATGGATCTGTGGTGTTTGGGGAGCCAGGTTAGAGCAATTGGCTGCACTGGCCTCCCACTAATATA ACCTGCTGTCAGAAGCCTGACTCAAGCCTCACTCCTTGCATAGCTCCTGAGGGGAGAACCGTGGAGCCAT TCTCCTCCCAGCCTCTAGTCTAACGGAGCATCACAAAGAAGAGAAAAAAAATGTCCTTGTATTGCTATTG GTTTTCCATGTTTATCTTAAGGGTACTATTAAAAAAGATTTTACTACAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC076605
• Insert Size: 1842 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC076605, AAH76605
• RefSeq Size: 2727 bp
• RefSeq ORF: 1842 bp
• Locus ID: 232223
• Cytogenetics: 6 D1
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is evidence for additional isoforms resulting from the use of a non-AUG (CUG), and an in-frame downstream AUG as translation initiation codons (PMID:20018845). [provided by RefSeq, Aug 2017]