Dgcr8 (BC062919) Mouse Untagged Clone

CAT#: MC206166

Dgcr8 (untagged) - Mouse DiGeorge syndrome critical region gene 8 (cDNA clone MGC:86132 IMAGE:6833280), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dgcr8
• Synonyms: N41, Gy1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC062919
  GCGGGTTGTGGCGGCGCCGGGGTCGGTGAGGGTCGACCGGCTGTGGTCGGGCTGCGGGCGGCTCGGGCAG GTCGCGGGCGCCACAGGTGGAAGAAGAAAGGTGCCACTTTGGCATGAAGATAGACTCACTTAGACGTCAA TCTTTTAAGCTGAGTGCATTGTGATTTCCAATAATTGAGGCAGTGGTTCTAAAAGCTGTCTACATTAATG AAAAGAGCAATGTGGCCAGCTTGACTAAGCCGCCAGTGTGTACAGCGCGGGCAGGACGACACCGGGTCTC GACGGACTTGTGCATGTTAGCAGTATAGATTTATGTAAGGTGGTTTAAAACTCTGGTCTTTTAAAGTAGT CTTAACTGCTCATAATATGGAGACATATGAGAGTCCCTCTCCTCTCCCGCGTGAGCCCGCAGGAGAAGCG ATGATGGAGAACCGAGCTTGCCCCTTCCAAGTGCTGCCCCATGAACAGTCTCCACCACCTCCCCTGCAAA CGTCCAGTGATGCAGAGGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACCTCCTGCCGACGA CCCATTCAACTTCTACGGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGAC CCGAACTGTAGTGGCCACAGCCCGCGCACTGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACC TTAAGTTGCTTAAGGATGTAAAGATTAGCGTGAGCTTTACTGAGAGCTGCAGGAGTAAGGACAGGAAGGT GCTGTACACAGGAGTAGAACGCAGCACTCGGCCTGAGTGTGGCCAGCTCCTTAGTCCTGTCAGTGGGGAC GTGCATGCTTGTCCCTTTGGCGGGAGTGTTGGTAATGGGGTAGGCCTAGGGGGTGAGAGTGCAGATAAGA AGGATGAGGAAAATGAGCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGATTT TACTGACAATTTGGAGCTAGATGAAGAAGGAACAGGCGGGTTCACGGCTAAAGCAATCGTTCAAAGAGAC AGAGTGGATGAAGAGGCCTTGAATTTCTCCTATGAGGATGACTTTGACAATGATGTGGACGCTTTACTAG AAGAAGGTCTCTGTGCTCCCAAGAAGAGGCGAATGGAGGAAAAATATGGCGGAGACAGTGATCATCCATC TGATGGAGAGACAAGTGTACAGCCAATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGTCCA CCTACAGAGCCATTGCCTGATGGATGGATCATGACTTTTCATAATTCTGGAGTCCCTGTATACCTGCACA GAGAGTCTCGAGTGGTCACTTGGTCCAGACCCTACTTCTTGGGAACAGGAAGCATACGGAAACATGATCC TCCTCTAAGCAGTATCCCCTGCCTACATTATAAGAAAATGAAGGACAATGAGGAACGAGAACAAAACTGT GATCTTGCCCCCAGTGGAGAGGTGTCACCTGTCAAGCCCTTGGGTCGGTCTGCAGAGTTGGATTTCCCTC TGGAAGAGCCTGACTCCATGGGTGGAGACTCAGGGTCCATGGATGAGAAGGACCCATTGGGGGCTGAGGC AGCCGCTGGAGCCCTGGGACAAGTGAAGGCTAAAGTTGAGGTGTGCAAAGATGAATCAGTTGATCTGGAG GAATTTCGTAATTACCTTGAGAAGCGTTTTGACTTTGAACAAGTAACTGTGAAAAAATTCAGGACTTGGG CTGAGCGGCGTCAGTTCAACCGTGAGATGAAGCGGAAGCAGGCCGAGTCAGAGAGGCCCATCCTGCCAGC CAACCAGAAGCTGATCACTCTATCTGTACAAGATGCACCCACAAAGAAAGAGTTTGTCATCAATCCCAAT GGGAAGTCTGAGGTTTGCATCCTGCACGAATACATGCAGCGTGTCCTCAAGGTCCGCCCTGTTTATAATT TCTTTGAATGTGAGAATCCAAGTGAGCCTTTTGGTGCCTCCGTGACCATTGATGGTGTGACTTACGGATC TGGAACTGCAAGCAGCAAAAAACTTGCGAAGAATAAAGCTGCCCGAGCCACCCTGGAAATTCTCATCCCT GACTTTGTTAAACAGACCTCTGAGGAGAAGCCTAAAGACAGTGAAGAACTGGAGTATTTTAACCACATCA GTATTGAGGATTCACGAGTCTATGAGCTGACAAGCAAGGCTGGGCTGTTGTCTCCATATCAGATCCTCCA TGAGTGCCTTAAAAGAAACCATGGAATGGGTGACACATCCATCAAGTTTGAAGTGGTTCCTGGGAAAAAC CAGAAGAGTGAATATGTTATGGCATGCGGCAAACACACAGTGCGCGGGTGGTGTAAGAATAAACGAGTTG GGAAACAATTAGCATCTCAGAAAATCCTTCAGCTACTGCACCCACATGTCAAGAACTGGGGTTCCTTACT ACGCATGTATGGTCGTGAGAGCAGCAAAATGGTCAAGCAGGAGACCTCTGACAAGAGTGTGATAGAGCTA CAGCAGTATGCCAAGAAGAACAGGCCCAACCTTCACATCCTGAGCAAGCTACAAGAAGAGATGAAGAGGC TGGCTGCAGAGCGGGAGGAGACTCGGAAGAAACCCAAGATGTCAATTGTAGCATCTGCCCAGCCTGGTGG TGAGCCCTTGTGCACAGTCGATGTATGAGGTAGGCAGCATGGGCCAACAGTGCTACCCAGGAGAGACCAT CAGCCACACATCATCACTCTGCAGCTCCAGGCCTCCAACCTAAGTTCCTTCCCTGTGGCAGGGTCTGGGC TCTGGCTCTGGCACATGGGGACAGCTGTGACTACACAGTACACATGCAAAGAAGCAGTTCTGGACAAGCT GCTCTCAATGTGACTGGATATACTTAAGGATCAGTCATAAGTTAACTGCACAAGTGGAAGCTGATAGACA GCTGTAGATCCTGCTTGTATGTATCTGCTGCGGACCGTTTTTATGAAGGTTTTCATTAATTTTAGTACAC TATATGCACTGACAAGAAGTAATACTTCGTTGATAGATGAGATGACCAGGTTTTATAGCTTTGGCTGGCC TATGATGCCAGGTAGCCCCTTTGCTATGGTTCTTTCTGGTGGAGCCACTGAGACTTGGTCAGAAGATGCA GGTATCCTGTCCTGTAGAATTACCTGCTTTGTGTGTTTTGCATTTTCTTCTGAAAAAGTTATAACAGAAA GGAATATTTCAGGCTATTTTGGCTTAAAATAAAACAAAATTTTAGCACCAGAAATACTCTTCCGAGATTG CAGTGAAGATACTGAGTGTTAGTCCGATTGATCCTTTTTCTTATGAAGTCTTAATGTCCTAACAGCCGTC TTCTGGATACTGAAGCTTCCACCTTCGATAAACTTAGCAACAATTTTTTACAGAGATTTTTGCACAACCA AGCCCATCTGTTCATCAATTTTTAAAGCTTTTATGATGTTTTAAAATTTGGAAAGAAACTTTTACAGTAA GAATGAAAGATGCATTTCAACAGATGAATATGCATAAATAGGGAACTGGCCTTCAATGTAGCCCATCACT GACCAAGAGCTGCCTGGCACATTGTACACGATAAGTAGTTATGGTGTTTTTTGATCTCAAGGATAGCTGC TCTGTGCAGCCAGGCCTGATTGCAGGTTTTTGTCTACCTATGCTCATTGGATAGCCCCTGTAAATGGGTA TTGAGTAGAGGGGTATTCAGAGCCCTATTACCTGTTCAGAGGGGCTACACTCTACATCCTCAGTGTCTCT AACCATGTACCTGAAGATACATGGGTGACATGTGCTGTTAGAAAGATAAGATGGCAACTGTGGGCCTCCC AACAGCATCTTCCTCCTCCTTAATCATGTGTGTGACAGCAGTTAATGTTTATGTTAATCTTGGCAAAAAG AACCCTTAATTCAAGTTGGTAGACTTGGACACTTGATTCTTTTCTGCCTCCCATTCAGTTGTGTTCAGTC TTTTGACTAACCATTGCCCTTAAAACATACTTAACATTGAAAAAGCATATGGTAATGGTGGTATCTCTGC AGCCTCACCGACCCAGGCACTGTCAACAGGTGCTGCCACACATTCCTGGTCTCCAGTGCCCTCCCTGTCT CTTACAGGTGGGTCTTATATGTACAGTCATGCTGCAAGGGTGGGGAGCCTGTGCTGATGTGCTTCTGTGT GTTGGCCAAGGCTGTAGGGTGTACCCTGATGGTCTGAACACATAAAACTGTCCAAAAGGGAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC062919
• Insert Size: 2322 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062919, AAH62919
• RefSeq Size: 4297 bp
• RefSeq ORF: 2322 bp
• Locus ID: 94223
• Cytogenetics: 16 11.31 cM
• Gene Summary: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983).[UniProtKB/Swiss-Prot Function]