D12Ertd553e (BC052465) Mouse Untagged Clone

CAT#: MC206151

D12Ertd553e (untagged) - Mouse DNA segment, Chr 12, ERATO Doi 553, expressed (cDNA clone MGC:63391 IMAGE:6834768), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D12Ertd553e
• Synonyms: 2410157M17Rik; 9630047E15; D12Ertd553e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052465
  CGACTCCGTGGGAAGCAACACTGAGGAATGCAGGTGAGCTGAGGCGCAGAGAAGAGGGCTCTGAAGAGAA GCACCCTGAGGAGCACCACTCCCATAAGGACAGAGACCCTCGGGGACCACAGACTTGATTTGGAGAGCTC TTTCCTGTGGCCTCATAGCTGCTCTTCTGCTTTGTCCCTTCAGTCTGGATTGGTGACACACACCCACTGG AGATTATTTCCAGGTTTGCTGGCATGGGAAACTTGCTCAAAGTCCTGACCCGGGAAATTGAGAATTATCC ACACTTCTTCCTGGATTTTGAAAATGCACAGCCTACAGAAGGAGAGCGAGAGATATGGAACCAGATCAGT GCTGTCCTCCAGGATTCTGAGAGCATCCTCACAGACTTACAGGCTTACAAAGGGGCAGGACCAGAGATCC GAGATGCAATTCAAAATCCCAATGACATTCAACTTCAGGAGAAGGCTTGGAATGCAGTGTGTCCTCTGGT GGTGAGGCTGAAGAGGTTTTACGAATTCTCCATCCGTCTTGAAAAAGCACTTCAGAGTTTATTGGAATCT CTGACGTGTCCACCCTATACACCAACCCAGCACCTGGAACGGGAGCAAGCCCTCGCAAAGGAATTTGCAG AAATCTTACATTTTACCCTTCGATTCGATGAGCTGAAGATGAGGAACCCCGCTATTCAGAATGACTTCAG TTACTATCGAAGAACAATAAGTCGTAACCGTATCAACAACATGCATCTAGACATTGAGAATGAAGTTAAT AATGAGATGGCCAATCGAATGTCCCTCTTCTATGCAGAAGCCACACCGATGTTGAAGACTCTTAGCAATG CCACAATGCACTTTGTCTCCGAAAATAAAACCCTGCCAATAGAGAATACCACGGACTGCCTCAGTACGAT GACAAGCGTCTGTAAAGTCATGCTGGAGACACCGGAGTACAGGAGCAGGTTTACAAGCGAGGAGACACTG ATGTTTTGCATGAGGGTGATGGTGGGCGTCATCATCCTCTATGACCACGTGCACCCCGTGGGAGCTTTCT GCAAAACATCCAAGATTGATATGAAAGGCTGCATAAAGGTGTTGAAGGAACAGGCCCCAGACAGTGTGGA AGGGCTCCTGAATGCTCTCAGGTTCACCACAAAGCACTTGAATGATGAATCGACTTCCAAACAGATTCGA GCGATGCTTCAGTAGAGGCCTGCTCAGAGAGGAGGATCTATGCTGACCTCAGAAGATGTAGATGTTTACA TAATTTAATACAGATTGATGTTAATACTTGTGTATTTACATAACCATTTCCTTCTTGTCACTGAAATATA TGGACCTGAATTTGTATCCTGACTGATTCAACCCAGCCGAGCATAAATTGACTTGAGCGCCTTACCTTTG ATGTCTGAAGCGAAACTCCTTTCACCAAGGGCCAGATTCGGAAACTTCAACCTTGGCTGCTGGAGTCCTG TAGTGATACCTGTAACAATCAGAAATTCTTAATCGCTTGTGGTATGGTTTTAATTCTAGATGTTTTATCT CTCTGGGAGTATAGTTTATAGAAACACAAAGTACTTGATTTCCTGTGTCGGCTCAGATACAAGAAACACA CACAACTGTTACCCTGACAGAGAACAGAGAGAGAAGATCCAAGATAAGAAAACTGCATGTGGAGAGCGGC GCACAAGAGTTCTGAGTTCAGCACGTCCCCCACCCTCTTCCTGCCGCCTGCAAACCTCACGTGCAGCTTT TCTATATGCACGTGTTTTATTAATCTGAATATTCTCCTTCTGTCTCGGTTTGTGTCCCTTTCATTCCCTT TTAGTATTTGCAAGAGGAAAATTGTCTGTGTTTTAATCAGCTAATGAGGAGGCTATGGGATGGACAGAGG GCTTCTTGCAGGAGAATTGAGAAAAGTGACTATCTTAAGAATATTATTTCTTTTTTTAAAAAAGAATATT AGAGAGAAAGAGAGAGAGAGAGAGTTGGCATAAGGACAGTCTTATCTGTGTCAACCCTCTGCCTGCAGGG ACAGATAAATGGAACTCTGGTTGTGATATCATAACTAAGATCAGTCACCTCCCAGGAGTCTGCAGAGTTC TAGGTACACCACCCGTCTTAATATGGGAGCTCCCATTCTGTATCAAAATGGACTGTTCATATCAGAGCTG AGAACTAAAGCACTTCGTTCATTATCAGAAAACTGGAGGAATTTAGAGAGAGGAAATCTACGATTTCTAA TTACCTGGAGTCAGCCCCCACTAGTCTGAGTACTTCGGCAAATTCACTTTTTGTCTCACCTTCATCATAT GGCTCAGTGGTTAAGAGTACCTGTTGCTGTTGCAGAGGACCTGGATTGGATTCCCAGCACCCACTCAGTG GCCCACAACTATCCAATACTCCCGATGCATGGGATCCAGTGCCCTCTTCTGATCTCTATGGGCATCAGGC ATGCACACAGTACACTTATATACATGCAGAAAAAAATACTGAGACACATGAAATAAGATAAATCTTTTTT TTAAATCTAAAATGGAAGGCTACTAGGAGCCACTACACTAACCTTTCTTCATGCATAAATCCAATGAGTA CTACCCCACCCATCTTGATGAATATCAATATCTTCAGGACTGCAATCATTCTGCCTATAGGTCAGAACTG AGCTAGGATGGAATCTTGAAACATATTCTGACCTCCAATGAAAAGATATGGGCATACAAGGTGAGTTGTA CGTAGAACTAAATCCTTGAGTGTTTCCCACCTGACTCTACTATACTCTTCCTGATCACCATGTGGCCTAA GAATATATACTATGGAGTCTATGGACCACTGTAGAGAAGTTTTTAGTCTAGCCTGTTTGATTACTTGTAA ATCTCTGCTAGAAGGAGAGAATTTTCCAGACAACTGAATGAATGATAATCCTGGAAGTCAGGTCTAGGAT GATTAGGGTGTGGTATTATTTGGTTTGCAACATCCCAAGGCAATACTTCCAGACTAAGGTGGCCATCCAG CATTCCTTTGAGTCCTCCAAGAAAGATGATACTTGGTTCATTTCAAAAAACCAAATGCAGACAAATGGGA GAACCAGTTCCCCAGCTTATGTTAGCATAGTCAACAGCGAGCATAAAGACTTTGTCACTTGGAGCGTTAC CAGCAGAGTAAATGTGGCCATTGACTGGCCTAAGCATTCTCTAAGAGTTGTGCTAGATGGCTTCCATGAT CTTGTCAAACCTAGAAATTCCAAAATCTCGAGGAAGCTAGAAAACACAATCCTGTGGCAGTAAGACATTC TCCAGTGTTCAGCTTTAGGGATTTTCTATTTGGCAGCCATAGGCAATGCTTATTTGGGCAACTCTCACGC CTACAAGAGCAGAGATGCACAATAAGCATTTGTCCATTTGCTCTTTCCTCAACAGAGGAGATTAGGATTT CAAAGAACAAAGAACTGCCCAGTTTCTATTGGGCTCTTAGACACAGACCTGAGCCATGAACCCAAGCCAC GTATCTACACCATAGCTGCCTCTCAGCTGGCTACATAAGGAAACATTAGGGATTAACTGTCAGAATATAC ATACATACATACATACATACATACATAAATAAATAAATAAATAAATAAATAATAGCTTAGGACATTTGTA ACTCTTAGTTAGACTTTCTATTAAATGGGACCAAATGTGTGTATGGGTGCTGTCAGTCTGCTTCAGTCTA GCCAGTCCAGTATCTCCAAGATGAGACTCAACAGGACTTGGATTTTGAGAAGCCCAGGCTCTAAATAATA GCCTCAGGTCATCAGGGGGACAGACTGAGTGTCTGTGATCACTAACAGGGAGTGGGCCTTGAGTCCACTC CAGAACTGTGGTGGGAGACCAATACTCTTAACAGACTGTCCAGTAGGTATCGGGAATCCTTGAACAATTT GGGTTTTCTTCCCCTTTTTTTCTCTTTCGTGCTGCATGCACATTTATCCAACACTCACGGGTTGTTTGGG AAAAAGTACCAGCAATGATGTCGATGTTAATAATACTTCATAATTGATATCTTATAGCTTTCAAGTAAAG TGTTTTCTATAACTCAGATAATTAATTTGAAGCAAGAAAGAAGGTAAAAATGAGGTTTCCCAGCCATGTT GCTGCTTTTGGCATGGTCGCTTCTCTGAGGTTCACCTAGGCTTTCACAGCAAAAGTTGGGTGGAAAGAGA AATTTTTATTTGTGATGACACAATAGAGCCAGAATATTAGCTAACAACGTTGAAGGAGGTGTTGGGTGTT TTTAAATATTTAGTTAATATATTCCTCATGGACATAAAATACATTCTTAGTTATTATCATTATGTCAAAC AAGAATGGCAAGCCTAAGTTTGTGAGGCATCATCACAATTCTGTCAAAATAATGTTATGCTTTTGAAAGA TAAAGCCTGCTCAATCTAGGGATCCAGTTGTGCAGTAGAGTTCAGACTTCTCATCCAATCTTATATTGTA GGCATTTCTCAAGTGTTTTAGATATTAATAACTAACATTGGGATTTTACTTTGCCAGCCCCAGCCTTCAG AACTCCATTGTCATAGTACACCTGTAAAAATGGTTGCACATCAGAAGGGGCTTTGAAGCGCTTGTATGTT GTTTGCCGGAGTCATTGTAATTTTTTTGTTTGTTTTTTTGGGCTTTTTGTTTGTTTGTTTTTGCCTGCAT TGCCTCTTTTTGTACAGAACTTTTGAGTGTGAAATGAAAATTAAAGTTTGGTACATACACAGTTTAAAAA AAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC052465
• Insert Size: 972 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052465, AAH52465
• RefSeq Size: 4781 bp
• RefSeq ORF: 972 bp
• Locus ID: 76820
• Cytogenetics: 12 5.63 cM
• Gene Summary: May negatively regulate RAC1 signaling and RAC1-driven cytoskeletal remodeling. May regulate chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions.[UniProtKB/Swiss-Prot Function]