Ipmk (BC052463) Mouse Untagged Clone

CAT#: MC206150

Ipmk (untagged) - Mouse inositol polyphosphate multikinase (cDNA clone MGC:63346 IMAGE:6834748), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ipmk
• Synonyms: 2410017C19Rik; AA408208; Impk
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052463
  GGGGCCGCGGCGCCCTTATCCCGGGCGCGGGCGGCCGGGGTTCAGAGTGCGGGCCGCGGAGCGGGCAGCG CGGTCTCGGCATGGGTCCGCGCGCTGGGCCTGCGCGGGCGATGCCGCCCGGTCCTCCCTGACGCCTGACG CCGAGTGTCGCCGCCCCGATGGCCGCCGAGCCCCCAGCGCTGCGCCTGCGGCCGCCGGGAAGCACCGGAG ACAGCCCGCCGGTGCCGCGCCTGCTCGGAGGCTGCGTGCCGCTGTCGCATCAGGTGGCTGGCCACATGTA CGGCAAGGACAAAGTGGGCATACTCCAGCACCCAGATGGTACAGTTCTGAAACAGCTACAGCCACCGCCA CGGGGCCCAAGAGAGCTGGAATTTTATACCATGGTTTACGCTGCTGACTGTGCCGATGCTGTTCTCCTGG AGCTGCGAAAACACCTGCCCAAATACTACGGCGTCTGGTCCCCTCCCACCGCACCAAACGATGTGTACCT AAAACTGGAAGATGTGACTCATAAGTTTAACAAACCCTGTATAATGGACGTGAAGATTGGGCGGAAGAGC TACGACCCCTTTGCGTCATCAGAGAAGATTCAGCAGCAGGTCAGCAAGTACCCTCTGATGGAGGAGATCG GGTTCCTGGTGCTCGGCATGAGGGTTTATCATCTTCACTCTGACAGCTACGAGACACAAAACCAGCACTA TGGAAGAGGCCTAACGAAAGAGACCCTGAAGGAAGGCGTCTCCAAGTTTTTCCACAATGGCTTCTGTTTA AGAAAAGATGCGATTGCCGCCAGTATTCAGAAGGTAGAGAAGATTCTCCAGTGGTTTGAAAATCAGAAGC AGCTTAACTTTTACGCAAGTTCTTTACTGTTTGTTTATGAAGGTTCATCTCAGCCAGCTACTACAAAAGC AAACGATAGAACTTTGGCGGGGAGGTTTCTCTCCAAAGGACCCCTGACGGATGCGGACGGCCTGGAGTGC AATAACAACTTCCACCTGTTCGGCGCCCCGCCCAACGGGATGTCGGTGGGCAAGAGCTTATCGAAGGCGT ACTCGAGGCACAGAAAGCTGTATGCAAAAAAGCACCAGAGTCAGACTTCCCTGAAAGTCGAAACGCTGGA GCAAGACAACGGGTGGAGAAGCATGTCCCAGGAACACTTAAACGGAAACGTGCTTGCCCAACTGGAAAAG GTGTTCTACCACCTTCCCGCGGGTCGTCCGGAGATCCCGGAAGCGGAAGTACGGATGATAGACTTTGCTC ACGTGTTCCCTAGCAACACAGTCGATGAGGGGTATGTTTACGGTCTGAAGCATCTAATTGCCGTGCTTCG GAGTATTTTAGACAGTTGAGTCTTGCTGCAGTCTGTGAAGGAGCGGGCCAATCACCATGGAGAGCTGCAG GCGTTAGCCGAGCACCTGTACTGCTCTGTAAAAAATTTGTATTGTGAGTCAGCGTTTTATTTGTCTTTAT ACTTTTGGTAGATGGTTAACTTTTTTATATTCTTACTCAGGAATACTAATTTGTTCATTAGAAAACTATG AAGAATAAAGAAAAAGGAATGTCGAGCAGGGAGTGTGTGGAACACGGAGCAAACAGCTGTAACACTCGCA AATCATTACGCAGTCATTTAGAGGTCACATAGCTTTCTGTAGCCAGTCACCATGAAATCTGTCCACCCAA CCTTGCCAGTGAGCCGACCCTAAACTATGACTTTATCAGACCATCCTAAGATCTAGAAAGGTCCCTAAAA CTGGAGAAGATTTTACACGGAAACGAGGCTCTGTGGGTTCTATATTAAAGGCAGAGTGCAGCGCAGCGAA TGAAAGAAAGGTGCTTAAGCCAGCAGGGCTCCCTCCCGGTCCTGTCTGCTCTGTGCTGAGCATCATGGGA AGGGGAGTGTCAGGTGGCCGGCAGCAGCTAACAGCACAAATTTTCCTCCCTAGGGTCAAGAGAAGAGAGA TATTTGTAAAGAACATATTACCCTTTTCTTTCTTTTGTTTTAAAGAATTTCATTTATGGAAAAGTCTTCA TTTACTATGTTGTGAAAGCTGTCATAAAACACCCTCTTTTTATTACCAGTGAGGCTCCCGAGAGGTGAAT GGTGGAAACTTTGTTTTATCTCTTCTTTAATTTTTGAGAGTCTTCCATTTTAGAAAATCCATTCTTTAAA ATGTATCCCCATTGACTTTTTAAGCCTTAGCATAAACATTATCTCTCAGAATTTGGTTAAGCATCCACAG AACGCTGTGCCCAGTGCACTGGAGGAACCCTGCTTAGGTCTCACAACAGAACGCTGTGCCCAGTGCACTG GAGGAACCCTGCTTAGGTCTCACAACAGAACGTTGTGCCCAGTGCACTGGAGGAACCCTGCTTAGGTCTC ACTCCCGTGGTGTCTAACAGAGAGGTCCTAGTTAATTTCACTCGGAGCAACAGGCAGGCAACTTTTCCTT GAAAAAAAAGATATTTATAAGTGTCATGTTTATTTTACCACTACCACCTTTTTCAAAGCTGTCGTTAAAG TGTTTTCAAAACTGAAATTAAATTTGCAAATCTTTTTGCTGTGCTTGCTTGCCCTTTGACCCGCTGATGT GATAAAATAACTGAACAGCTTGTCCTTTTTAGACAAGCTTTGAGTCACGACCTGACCTTTTTTTCCTTTT CACCTCTTTTTTCCTTGAGGTTTACAAATCAAACTGCAAAATCCAAGAACCATTTTCTGTAATGTGGAGG AACTTTTACAGTCCAAATGTAGTTTGCAAATGCCCACGCTCAAATGTGAGCCGGAAAGGAATGGGTCATT AACCAGAGTGGGTCTGGTTAACGTGCCTTTTAGGGGTTTTCTCTGGGAGGCTGCAGGTGTTCTCAGACCT GCAAGAACGACACATTCTCTGCCCAGGTGTGGACTGGCTATACCTATTTTTGTTTCTCTCATTTTGTGGG AGTGAATTTTAAGTGATAATTATTAGAAAACTTTCAGTTTGACTGTTTTTCTTACAATATTGACTTGTTA TCTGGGTGTGATTCAGGAACTCTCAAGCCCATCTGCTGAACACTATTTTGGCTCTTAAGAGGCAGTTTGA GATGGTATCAGCCTATATATGAGGAATTGGGAAACTTGAGTATTCGAAGCTTTGGGCACACTTCAGCTTA GTAAAGTCTCTGATCTACAGCTGTTTCAAACATTTCGGAAGCCAGCATCCTGCCTTTTCGACAAGTAGGT TTTGTCTGAATAAAACAGGAAGTGATTCTTCCCCCTGCTTCTCAGGGAAGGGGTGAGCATCCAGTTCTTC TCGTTTACAGTTTCCCAACTGCTGGCCACTTTGTGTTACTTTGTGCTCCTTGATGCCATGATCAACATGT TCGTGATGTAAGCAACCTGTGGTGGGTGTTTCCCGGTCTGTCCTGTCTTTAAAAATGATGCTGAGATTTG CCACTTTGGGGAAGAGGCATCTGTCACTAACCATGCATATGTGAACACACGGATATTTAAGAAATGAGCA AATGAGGAGGTGGTTTGAACTGGTGGTGATGCTAGCCGCCCCCTCTTAGCCCCCCCAACATGATTTGAGT GTGTTCCTTATGGGATGCACTCATGGAAAGGACTTGTGGGTGTGAATACATTTCTCAAATAAAAGTTTAA ATTGTAAAATAGTTTTATAATAAAGTATTTACATTAATTATTTTAATATGGATGGAAGTTGCATAAATTA TAATTGTGAACTATTTAGTCTTTCAAGAGAGTTTTGAAAAATGTATGTAAGTGGCTCTGATGGCTGTGGT GTTAAATTAGTATGCGATGCTTTGTTCTGTGTGTTTGGAGGGGGCTGGGCACTCTACCTGTCCACACTAC AGACAGACAGTTACTGCGGGCCGAGTGAGAACCGATTCCTGAATGCCTGCCGGCGTCCTGTGATGCAGTT GCCCGAGATCTCCACAGTGTTGCCCTCGCTGTCAGGCCTCTGAACTGTCGAATTTATAACATTTGGGGGG AAATGAATTTCTATAATTACCTCTGTTGCAATCTTTTTATTGCTGTTAGGATTTGGAATGTGTAGGCAGG AGGAAGGGCTGAACTGGGAACTGACTGGCTCTGACTGTTAATACCACTCCCCTTGAGGTCTGATGTCCAG ACGTGCTTGGTTGAACCAGCCGTTTACACAGCTGTCCTTCTCTTGCAGACCCTCAACCGACCGTGGCTTT TGTGTATCCATAGCTTCCGTTGCCCTTTGTCTTGCCCCCTACAATGGTTCAGTTACCCAGAAACATCAAG TCCCTTTTAAGAATAGTCCATTTGGTTACTCTCAGGGTAGTGTCTGGGGGCCTTGGCCTCTCAGTTCACT CAGACTCAAGTCTGCACCTCACCAAGTATTTTCAACCCCTCTCCCTGACGTGTGCGTTGGTGAAGCAGTC AGGACTGATCGTCTCCAGCACCCTGTCTAGGTGGAGAGGCTCACGAGGACGACTTTAAAACAGAGACACA CCAACACCTGTATTTCAAGCTGTTAAAGCTAGTGTTTGGGTAAAGATGAAGTGCTAAGTATTGAGCTACA ACAGAGGCAGATGATTGGGGGCTGAGATCAAGGGAACCGCTGACTCTCCAGCTGGGGCAGTTACAGACAG CCTAAAACAGCCTGAAAAAGAGCGCTCTTAAGACATGCTTGTAAAGGTACATTCAGCCGTCGCCTTTGGA ATAATAAATAGCACAGTGTGACTATGGTTGCTCCTCGCTGGTGCAAACCTTTTAAATCTCAAGTTTTCAG ATTGCTGGGATTTCAGACTTGTCAGCGGACATGAAGGTATTTCTTGACTTCAGAGCAACTCTGTTGTTAT AACATCCAATACTGATATCTGGAGACAGATTCAGCCTCTTGGCTGTCGATAGTGTGTCTTCCCACTTGCT TCGATAGCTTCTATCAAAAACCACCATGATTCGTGCCAGTAAATCAGACAGTCAAATTAAAAAAAAACAA AAACAAAACCAAAAACAGTAGAAACGTGTGGTGTGGTGAAACTTTGGCCTGGTGTCCACTTAAAAGATTA AAAGGTTAGTTATTGAAGATAGGGTTTCTGGTCCCCTGTAGCGAAAGGCACACATAGACACTTGGCTGTG GGATCTGTGGTGGAAGATGGGAAATCACACCCAGTTGTGTCCTTAGTAACATCCAGGCAGCAGTGCCAGT CCCCGCCCCCTGTTACTTGATGAATGAATGTATCCCTGTACTTTGGTGGAAATGTCTCTAGCTTTCTTTT TCTCAGTGTTTGTAATTTGTACTATTGATGGGATAGTCTTGGAGTGCAAGGCTCATCACTGTGTTTGTAG AATCATCTAATGTGATATACCAATTTTTATAAGCGATATTTAGATAACTCTAATAACTGTACATTCAACA ACTTATTAAAATGTTTTGCATTGGAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC052463
• Insert Size: 1191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052463, AAH52463
• RefSeq Size: 5432 bp
• RefSeq ORF: 1191 bp
• Locus ID: 69718
• Cytogenetics: 10 B5.3
• Gene Summary: Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) (PubMed:15939867). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate. Plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Binding of these highly phosphorylated inositol phosphates to MLKL mediates the release of an N-terminal auto-inhibitory region, leading to activation of the kinase. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis (By similarity). Required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6) (PubMed:15939867).[UniProtKB/Swiss-Prot Function]