Phf12 (BC043080) Mouse Untagged Clone

CAT#: MC206136

Phf12 (untagged) - Mouse PHD finger protein 12 (cDNA clone MGC:57991 IMAGE:6400617), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf12
• Synonyms: PF1, mKIAA1523, F630045O13
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC043080
  AGGGGACCGGGGGTCAGAGGTGCCCCCTTCCCCTCCTCCCCCCTCCCCCCGGGGCTGGGGGGGCCGGAGC AGAGAGCGCCCAGCCCGAGAGGTGGATGAATGTGGGAGAAAATGGAGACCAAGACGATCGTGTACGACTT GGACACGTCGGGGGGGCTGATGGAGCAAATCCAAGCTCTGCTGGCTCCCCCCAAGACGGACGAGGCAGAA AAGCGGAGTCGGAAGCCCGAGAAGGAGTCCCGGAGAAGCGGCAGGGCCACCAACCACGACAGCTGCGATA GCTGCAAGGAAGGTGGGGATCTCTTGTGCTGCGATCACTGCCCTGCCGCCTTCCACCTCCAGTGCTGTAA CCCTCCACTGAGTGAAGAGATGTTGCCTCCTGGGGAGTGGATGTGTCACCGGTGTACTGTCCGCCGAAAG AAACGAGAGCAGAAAAAGGAGCTTGGCCATGTCAATGGACTGGTGGACAAGTCTAGCAAACGGACTACAT CCCCCAGCAGTGACACTGACTTGTTGGACAGACCAGCTAGCAAAACTGAACTCAAGGCCATTGCCCATGC CCGGATCCTGGAAAGGAGAGCCAGCCGGCCTGGCACCCCCACATCCAACGCCAGCACAGAGACCCCCACC TCTGAGCATAATGACGTCGATGAGGACATCGTTGACGTGGACGAGGAGCCAGTCGCAGCAGAGCCGGACT ATGTGCAGCCCCAGCTGCGGCGGCCTTTTGAGCTGCTGATTGCTGCTGCCATGGAGCGGAACCCTACCCA GTTTCAGTTGCCCAACGAACTGACCTGTACCACTGCACTACCAGGTTCTAGCAAACGGAGAAGAAAGGAA GAAACCACAGGGAAGAATGTAAAAAGGACACAGCATGAGTTAGACCATAATGGTCTTGTTCCCTTGCCGG TCAAAGTCTGCTTCACATGTAACAGGAGTTGTCGAGTGGCCCCCCTCATCCAGTGTGACTATTGCCCGCT TCTGTTTCACATGGACTGCCTCGAGCCCCCGCTCACTGCCATGCCCCTGGGCAGATGGATGTGTCCGAAT CACATCGAACATGTGGTGCTGAACCAGAAGAACCTGACTCTGAGCAATCGGTGCCAGGTGTTTGATCGGT TCCAGGACACCATTTCACAACATGTTGTCAAAGTAGATTTCCTGAACCGAATCCACAAGAAGCACCCTCC TAACCGCCGTGTGCTCCAGTCTGTCAAAAGAAGAAGCTTGAAGGTTCCTGATGCTATAAAATCTCAGTAC CAGTTTCCACCCCCGCTTATTGCACCCGCGGCCATTCGGGACGGGGAGCTGATCTGCAGTGGGGTCCCTG AGGAATCACAAACGCACCTTTTGAACTCTGAGCACTTAGCCACTCAGGCAGAGCAGCAGGAGTGGCTCTG TAGTGTTGTTGCGCTCCAGTGCAGCATATTGAAACATTTATCTGCTAAGCAGATGCCTTCGCCTTGGGAC TCTGAACAGACAGAGAAGGCTGATATTAAGCCTGTTATTGTGACTGACAGCTCAATCACCACCTCCCTGC AAACCGCTGACAAGGCACCTCTTCCTTCCCACTACCCTTTGTCCTGCCCCTCAGCAGTGAGCACCCAAAA TTCCCTGGGCTGCTCTCCACCCCACCAACCCCCAACCCTAGAAGACATCAGCTGCAGTTCTTGTGTGGAA AAGTCCAAGAAAGCCCCTTGTGGGACTGCCAATGGGCCGGTGAACACAGAGATAAAAGCCAATGGCCCAC ACCTCTACAGCAGCCCCACTGATTCCACGGATCCCCGGCGACTTCCTGGCGCCAACACCCCTTTACCAGG CCTCACACACCGGCAAGGCTGGCCCCGGCCCCTCACGCCACCATCGGCTGGGGGACTTCAGAACCACGTC GGCATCATTGTGAAGACAGAAAATGCCACTGGCCCCAGCTCTTGTCCCCAGAGGAGTTTGGTACCCGTCC CAAGCCTGCCCCCTTCCATTCCCAGCTCTTGTGCCAGCATCGAGAACACCAGCACTTTGCATAGAAAGAC TGTCCAGTCACAGATAGGACCTTCGTCGACAGAATCGAGGCCTCTGGGCTCACCCCCAAATGCCACCCGG GTGCTCACTCCCCCCCAGGCTGCAGGAGACAGTATCTTGGCCACAGGTGCCAACCAACGATTCTGCTCAC CAGCGCCATCATCAGATGGCAAGGTCAGCCCCGGCACGTTATCCATAGGAAGCGCTTTAACCGTACCCTC TTTCCCAGCCAACTCTACTGCCATGGTGGACCTCACCAACTCACTTCGAGCATTTATGGATGTCAACGGA GAAATCGAGATAAATATGTTGGATGAGAAGCTGATCAAGTTTCTGGCCTTGCAGAGAGTACATCAGCTTT TCCCTTCCCGGGTCCAAGCTTCACCGGGCAATGTTGGGACACATCCGCTGGCTTCTGGAGGGCACCACCC AGAAGTGCAAAGAAAGGAGGTACAGGCCCGAGCTGTGTTCTGCCCCCTCTTAGGGTTGGGAGGAGCTGTG AACATGTGCTATCGAACCCTCTACATCGGGACAGGAGCTGACATGGATGTGTGCCTTACAAACTATGGTC ACTGTAACTACGTGTCCGGGAAACATGCCTGCATATTCTACGATGAGAATACCAAACATTATGAGCTGTT AAACTACAGTGAGCATGGGACAACGGTGGACAATGTGCTGTATTCATGTGACTTCTCTGAGAAGACCCCG CCAACCCCCCCAAGCAGTATTGTTGCCAAAGTACAGAGTGTCATCAGGCGCCGCCGACACCAGAAACAGG ATGAAGAGCCAAGTGAGGAGGCAGCCATGATGAGTTCCCAGGCCCAGGGGCCACAGCGGAGACCCTGCAA TTGCAAAGCCAGCAGCTCAAGCTTGATTGGGGGCAGTGGGGCCGGCTGGGAGGGCACAGCCTTACTCCAC CATGGCAGCTACATCAAGCTGGGCTGCCTGCAGTTTGTCTTCAGCATCACTGAGTTTGCGACCAAACAGC CCAAAGGCGATGCCAGCCTGCTGCAGGATGGGGTCTTGGCTGAGAAACTCTCTCTCAAGCCCCATCAGGG CCCTGTGCTGCGCTCCAACTCCGTTCCCTAGGCCATTGGCCTGGACGCCCACCCAAGACTCCTGCAATGC AAAAATGTACACGAACCAAGCCTGGGTGTTTTCTATACCAGAAACCCTCAACTACAATCTTTGCATGAAA TGAAGAAAACCTTTTGACTGTTTTTTAAGACTTTTTTTCTTTTCTCAAGTTCTAGGGGGCATTTGCACAT ATATTTGTACTCAACATTTCATGGGAAAGCGGCAGATCCGCGCTGAGGAGCAGCGAGGGCAGGGACAGGA GGCCCTGGTCTGGACACTTCCTCCAGCACAATCCCTTCCCCCGCCTCCTGCTCCTCCCCCTCGACCGCCT GCCCACTGTTGTAAAATAATCAGAAACTTGTTCTATTTTGTGGCAGTGACAATAGTTTTATATTAAAAGA AAAAAATACAGTTTTCATACAGCAAAATCTATACAATATCATTGTTTTATTTAATATAAAGATCGCTACC CACTCCTTTCCATGGTTCCCACCCTACAAGGACTTCCCCTCTCTGCAGCAGTTGCACTACAGGTAGCTAC TGTGTATATGGACAAATGAGAAATGAATCCTTTTTTCTGGCTGTCCATCTATTTTATTTCAAATAAGGAA AAGTGTATTTGGATTTTGTGTAAATACATCTAGTGATGGCATTTTTTCAATGTTTTTAAAAGCTGTGTAC AGTACATGTGGTAGAGTGTTTCTCAAATTGTCTATTGTAGCAAAGGCGTTTTTGTCGTAAACCTGTTCTG TGTCCTTTTTTGTTCTTACCACTTCTCTTCCTCCTCACCCCAGATACTTCCTCTTCCCCACAACCAATGT ACATAGTAATTGTAATGTTTTAGACTTGACAGAAACTTTCCTGTATTCTGTATATAAAAACCAAAAATAC TTCAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC043080
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043080, AAH43080
• RefSeq Size: 4015 bp
• RefSeq ORF: 3000 bp
• Locus ID: 268448
• Cytogenetics: 11 B5
• Gene Summary: Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional TLE5 complexes to DNA (By similarity).[UniProtKB/Swiss-Prot Function]