Eef1d (BC079855) Mouse Untagged Clone

CAT#: MC206134

Eef1d (untagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (cDNA clone MGC:105169, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef1d
• Synonyms: EF-1-delta
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC079855
  TGAGCTCGCAGTTCCAGGTTTTGGTTGCTGCGTCCTTGGCTGGCGTGAGGCCAAAGCAAAAATGAGGAGT GGGAAGGCCTCTTGTGCACTGGAGACCGTCTGGGAGGACAGGCACAAGTATGAGGGAGCCGAGCGGCGCT TCCACGAGCAGGAGGCCACACAAGTCGTCGCCGCCGCCGCCACTGCCGCCGCCACTGCCTCCGCCTCTGT CCAGCAGCTCCTGGACGAAGTGCCAGCTGTGAACGGGCCCAGCCAGGAGGACACAGAGGACACTGAGGAG GCAGAGGCCCCAAACACCAGCAGCAGGAGTGATCCCGGGAAGAGCCACGAGTGCAAGAAGCCGATACAGA AAAAGAGGAAGCGCTCCCCGAAAAGTTGGCTGGGCCAGGCAGACCTGGCCCTTGTGGGCCTCTCGGCTGA CCACGTATGGTTAGACAAGCCACTCTTCGACCAGGCAGAAAGCTCCTACCGTCAGAGGCTGGCAGATGTA GCTGCCCAGGCAGCCCAGCCGCCTGCTCTGGCCCCTCGGGGGCCCTGTACACATGGAAGCCATGTGGCAT GCCACCATGTAACCTGGGGGATCTGGGTCAACAAGTCTTGCTTTGATCAAGCCGAGCGGGCTTTTGTGGA GTGGTCTCAGTCCTTGCTGCTGGCTGCAGAGGGGAGCCATAGGCAGGGGACTCCTGACACAGGCCAGCAG GCTGTCACTCCTGACCTGGCCTTGGCCTGCCAGCCTTGCCCACCAGCCAATGGCCAGCCTCCTCTGGGCA GCCTGCAGGCACTGGTGCGGGAGGTATGGCTGGAGAAGCCCCGGTACGATGCCGCTGAGAGGGGCTTCTA TGAGGCCCTGTTTGATGGTCATCCCCCAGGGAAAGTGCGCCTGCAGGAGCGAGCCAGTCAGGCAGAGGGT ACCCGGAGGGGCCGCAGAGACCATCGGAGCTGCAATAACGTAGGAAACAAGAGAGCTGGGTCAAAACGGG CCAACGGGGAGGCTCCTCCTGCTTTTCCCTACTGGTACTTCCTACACAAGGACGCAGAGGCCCCCTGGCT CAGCAAGCCTACCTACGACAGCGCTGAGTGCCGCCACCATGCCGTTGAGGCCCTGCGCATAGCCTGGCGC CTAGAAGCTGCCTCCCTGGCTCACCGACCCACACCCCGTTCTGGCCCATCCATGTCCAGCCTGAGACCCA ACAGAAAAATGGCTACAAACTTTCTAGCGCATGAGAAGATCTGGTTTGACAAGTTTAAATATGATGATGC AGAAAGGAGATTCTATGAGCAGATGAACGGGCCTGTGACCTCCGGCTCCCGCCAGGAGAATGGTGCCAGC GTGATCCTCCGAGACATTGCAAGAGCCAGAGAGAACATCCAGAAATCCTTGGCTGGAAGCTCAGGCCCTG GAGCCTCCAGTGGACCTGGTGGAGACCACAGTGAGCTCATTGTGAGGATTACCAGTCTGGAAGTGGAGAA CCAGAACCTTCGAGGCGTGGTGCAAGATTTGCAGCAGGCCATTTCCAAGTTGGAGGCCCGGCTGAGCTCT CTAGAGAAGAGTTCACCTACTCCCCGAGCCACGGCCCCACAGACCCAACATGTCTCTCCTATGCGTCAAG TGGAGCCCCCAACCAAGAAAGGAGCCACACCAGCAGAGGACGATGAGGACAAGGACATTGACCTGTTCGG CAGTGACGAGGAGGAAGAAGATAAGGAGGCTGCCCGACTACGGGAGGAGAGGCTACGCCAGTACGCAGAG AAGAAGGCCAAGAAGCCCACACTGGTGGCCAAATCCTCCATCCTTTTGGATGTTAAACCTTGGGATGATG AGACTGACATGGCCCAGCTAGAGACTTGTGTGCGTTCCATCCAATTGGACGGGCTGGTTTGGGGGGCCTC CAAGCTTGTGCCTGTTGGCTATGGCATCCGGAAGCTGCAGATCCAGTGTGTGGTGGAGGATGACAAAGTG GGCACCGACTTGCTCGAGGAGGAGATCACCAAATTTGAGGAGCATGTGCAGAGTGTCGACATCGCAGCTT TCAACAAGATCTGAATGTCCGAGAGAGTGTGGTTTCATGTGTGTGAGGACCTGCTGAGATTAAAGACTGA GACTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
• Restriction Sites: AscI-NotI
• ACCN: BC079855
• Insert Size: 1983 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079855, AAH79855
• RefSeq Size: 2174 bp
• RefSeq ORF: 1983 bp
• Locus ID: 66656
• Cytogenetics: 15 D3
• Gene Summary: Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.[UniProtKB/Swiss-Prot Function]