Ndel1 (BC046796) Mouse Untagged Clone

CAT#: MC206128

Ndel1 (untagged) - Mouse nuclear distribution gene E-like homolog 1 (A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ndel1
• Synonyms: NUDEL, MITAP1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046796
  CGGAGTGGAGCTCAGGGCTGCGGAAGGGAGCAGAGCTGAGCGGCTGGGCGGGCCTGGCCAGGCCAGCGGA GCAGAGACGTCGGCCGAGCGGCGGCGAACATGCGCTTTTGACACATTGGCGGCTTTCTTGATCATGGATG GTGAAGATATACCGGATTTTTCAAGTTTAAAGGAAGAAACTGCTTATTGGAAGGAACTTTCCTTGAAGTA TAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAGTTCCAGGAAGGAAGCAGAGAGTTAGAAGCA GAGTTGGAGGCACAGTTAGTACAGGCTGAACAAAGAAATAGAGACCTGCAGGCTGATAACCAAAGACTGA AGTATGAAGTGGAGGCGTTAAAGGAGAAACTAGAGCATCAGTATGCACAGAGCTACAAGCAGGTCTCAGT GTTAGAAGATGATTTAAGTCAGACCCGGGCCATTAAGGAGCAACTGCATAAGTATGTGAGAGAGCTGGAG CAGGCCAATGATGACCTGGAGCGAGCAAAAAGGGCAACAATAGTTTCACTGGAAGACTTTGAACAAAGGC TAAATCAGGCCATTGAACGAAATGCATTCTTAGAAAGTGAGCTCGATGAAAAGGAATCTTTGTTGGTCTC AGTACAGAGGTTAAAGGATGAAGCCAGAGATTTAAGGCAAGAACTAGCAGTTCGGGAACGACAACAGGAA GTGACCCGCAAGTCTGCCCCCAGCTCTCCAACTCTGGACTGTGAGAAGATGGATTCTGCGGTCCAGGCTT CACTCTCCTTGCCTGCAACGCCTGTTGGGAAAGGCACAGAAAACAGTTTTCCTTCACCAAAAGCTATACC AAACGGCTTTGGAACCAGTCCACTAACTCCTTCTGCTAGGATATCAGCACTAAACATCGTGGGAGATCTC TTGCGGAAAGTAGGGGCTTTAGAATCCAAGTTAGCCGCTTGCAGGAACTTTGCAAAAGACCAAGCATCCC GCAAATCTTATGTTCCAGGGAGCGTTAACTGTGGGGTAATGAACAGCAATGGCCCAGAGTGCCCAAGGTC AGGGCGAGCAACTTTCTTCCATAAAGGGGCAGTAAATGGCTTTGATCCAGCTCCTCCTCCTCCTGGGCTG GGCTCCTCACGCCCATCGTCAGCACCGGGTATGCTGCCTCTCAGTGTGTGAGTGCCCGGCCTCCAGGTGG GGCCCCTGCCCTCTTCCAGCAGCCCAGGACACCTACGCCTCGTCCCTCGGTGCCTGGGTCCAGCCCTGTG CCCCTCTGTCCGCCTCTTCACCGCTGGCAGAGGGCAGGCTGCATGCAGTGGCGGCTGCTGGGCCCTGCCC AGCCCCAGGACTCTGCGCGATATCAATACTGGCTATTTTCTCTTCTCGCCGTAGTGCCGTTGGTTTCACA TGATTGCACTTTTGTGGGTCACAAGTGATACATACGTGTATTACTCGATCACTGGATGTGGAAGTACCCA TTCATCACATCTGCCTCATAGCCCCCATCTTTGCTGTGCTGATAGGATTTAGTTGTGTTTAGGACATTGC AGATCTTCTAGAAGTTTCCCCTCAATCAGGTTGATAAGACCTTCTAAGCTTCTGCCAAGCATCTTCAGTG CTCACAATCATGTGTCCCCTAGCCTCACCCCTGCAGTTCAGGCCTGTTTCTGTCAGAGTCAGGAACATCT CTGAATTATGGAACATTGTCTGTACCTTCTGACTTTATGTAAGCAGTCCATTCCATTGCTTGTTTCTCAA AGCAGCGAGATACCTGTCTGAGCTGCAGGAGGCAGGGGCAGCCGGGACATGGGCACTTCAGGGCCGAGCC TCTGTGTTGCCTTCTCCTCTGTCCTCAGACTCAGGAGGCAGGTGGCAATCTCCTCGCCAGCATTCCAGGC CCTCAGTTCAATGACATCTCTCAGAGCATTTTTGTTTTCTCCTCTGAAGGTCCATCCAGTTGCATTGGGA AGGGTTGAGGGGCCTGATTCTGGGGCTGGCTAATGCCAGCTCTCGGGGGCAGACTGGCTAGGTGTTTGTC CCTTGCACCCTATGATTATTCTCATGCTGCACTTACTGTTTACATTTGTTTTATTGTACATAGGTTTGTA AACATTATTGCCTAAGATATTTGTATATAACTTGGGCTTTGTAGCTTTTATTTATTCAGACTCATATGGC ATGTTAATTAATGACTTACGATGGTGTCCTACTCTGGGCAGCTGTATAGGATCATCATGTGGTTAAAAGA AGATACTTCCCTCCAAAAAAAAATCTTTTAATGTGGAAACAATAAATTTCACAGAAAAAAAAAAAAAAAA AAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC046796
• Insert Size: 1038 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC046796, AAH46796
• RefSeq Size: 2318 bp
• RefSeq ORF: 1038 bp
• Locus ID: 83431
• Cytogenetics: 11 B3
• Gene Summary: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970).[UniProtKB/Swiss-Prot Function]