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Ccnd2 (BC049086) Mouse Untagged Clone

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SKU
MC206111
Ccnd2 (untagged) - Mouse cyclin D2 (cDNA clone MGC:61305 IMAGE:5716186), (10ug)
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  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ccnd2
Synonyms 2600016F06Rik; AI256817; BF642806; C86853; cD2; Vin-1; Vin1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC049086
CGAGCTGAGGAGAGCCGGGCAGTTCGGAGGGAAGGACCGGTGCGAGTCAGGCGGCCCTTGAGGCTCCGCT CGCCCACCTTCCACTCTTCTCTCTCTCTCTCCCTCTCTCTCTTTGCCATTTCTTTCCTCTCCCAAATCTC CCATTCAGCCAAAGGAAGGAGGTAAGGGAAGCACTCCCCGACCCCCCCGCACCTCCAAAAAATAATAATA ATAAAAAAAATTTACAGTCGGGACCGAGTGGTGGCCGGCTGGCTATGGAGCTGCTGTGCTGCGAGGTGGA CCCGGTCCGCAGGGCCGTGCCGGACCGCAACCTGCTGGAAGACCGCGTTCTGCAGAACCTGTTGACCATC GAGGAGCGCTACCTCCCGCAGTGTTCCTATTTCAAGTGCGTGCAGAAGGACATCCAACCGTACATGCGCA GGATGGTGGCCACCTGGATGCTAGAGGTCTGTGAGGAACAAAAGTGTGAAGAAGAGGTCTTTCCTCTGGC CATGAATTACCTGGACCGTTTCTTGGCTGGAGTCCCGACTCCTAAGACCCATCTTCAGCTCCTGGGTGCA GTGTGCATGTTCCTAGCTTCCAAGCTGAAAGAGACCATCCCGCTGACTGCGGAAAAGCTGTGCATTTACA CCGACAACTCTGTGAAGCCCCAGGAGCTGCTGGAGTGGGAACTGGTAGTGTTGGGTAAGCTGAAGTGGAA CCTGGCCGCAGTCACCCCTCACGACTTCATTGAGCACATCCTTCGCAAGCTGCCCCAGCAAAAGGAGAAG CTGTCCCTGATCCGCAAGCATGCGCAGACCTTCATCGCTCTGTGCGCTACCGACTTCAAGTTTGCCATGT ACCCGCCGTCGATGATTGCAACTGGAAGCGTGGGAGCAGCCATCTGTGGGCTTCAGCAGGATGATGAAGT GAACACACTCACGTGTGATGCCCTGACAGAGCTGCTGGCCAAGATCACCCACACTGATGTGGATTGTCTC AAAGCCTGCCAGGAGCAAATCGAAGCTCTGCTGCTGAACAGCCTGCAGCAGTTCCGTCAAGAGCAGCATA ACGCCGGATCCAAGTCTGTGGAAGATCCGGACCAAGCCACCACCCCTACAGACGTGCGGGATGTTGACCT GTGAGGAAGCCATTCGGGCGGCAAGAGAGAGGCGTGTTCGTCATCTGCTAGCCCCTTCTCTCTCTAGTTA TGTCTTGTTCTTTGTGTTTTTAGGATGAAACTTCAAAAAAGAAAAAAAAAAAACAAAAAACAAAAACAAA ATCTGCCCCCACCTAGATCCTATTTAAAGGTCTTTTAGAAGTGAGAGGAAAAGGCCGTATAGTTGTAACG CATGTGGTGCCTGTTCAAAGTCCTGCATAAGGGGATCCCTGTACACTCGAACCGTTATTATTTGATGATG TAAAAGTCATAGTGAGATGCTTACAGGAGAACCCGCAGACTAGCTAGAGAGTGTGCGTACCTGCAACATG GGAACGAATTAGAGGAGACTGTCTTTTGTGCTTGTGACCTAGTGCATATGCCCCCTTTAGTAGAATTGCA AGGAACCGTGGGTGCCAGGACCGCATGAGTAGTTTGCAAAGCAGTGAACCCAAGGAGGAATCAGAAACGA GAAGGGACATGCCGGGGAGGTTGCGCAAAACCACCCAACCACATGACTGAACCATTTTGGATGTAAGAAG CGCCTTGATGCCTCACCACGTGTTCCCAAGACTGAGCGTCCGGGTCTCTCCGTCGCATACTCTTGCTGAC TAAAGTAGCTGCTACCTAAGGGATATGTCTTTTGCCAGTTGGACACAGGTGAACGGCTCCTAAGTCTCAT GTTTGGTGAATTTGGGCTTCTACTTCCAAACATGGTTCATTGTAGATGTATTTTACTGTTATCTGATGGA GGGAATTCGCTATGGGCCATGACCAAAACGCATCTAAATGGGGGCAGATGGAGACCATAGACGGAATCTA AAACAAAGCTGCCTTGTGTAAGGGAAGAGGGTGAGGTGATCTTTAATCCCCTTGTTAAGAAAAAAAAAAA AAAAAAA
Restriction Sites AscI-NotI |
ACCN BC049086
Insert Size 870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC049086, AAH49086
RefSeq Size 2037 bp
RefSeq ORF 870 bp
Locus ID 12444
Cytogenetics 6 61.92 cM
Summary Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).UniProtKB/Swiss-Prot Function
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