Cfl1 (BC058726) Mouse Untagged Clone

CAT#: MC206105

Cfl1 (untagged) - Mouse cofilin 1, non-muscle (cDNA clone MGC:63415 IMAGE:5715707), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cfl1
• Synonyms: cofilin
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058726
  TCGGATCCCGGCAGCAGCTGCGGCGGCTCAGGTCTTCAGTTGCTCTTTCTGTCTCCCTTTCGTTTCCGGA AACATGGCCTCTGGTGTGGCTGTCTCTGATGGTGTCATCAAGGTGTTCAATGACATGAAGGTTCGCAAGT CTTCAACACCAGAAGAAGTGAAGAAACGCAAGAAGGCGGTGCTCTTTTGCCTGAGTGAGGACAAGAAGAA CATCATCCTGGAGGAGGGCAAGGAGATCCTGGTAGGAGATGTGGGGCAGACTGTGGACGACCCCTACACC ACTTTTGTCAAGATGCTGCCAGACAAGGACTGCCGCTATGCACTCTATGATGCAACCTATGAGACCAAGG AGAGCAAGAAGGAGGACCTGGTGTTCATCTTCTGGGCCCCCGAGAATGCACCCCTCAAGAGCAAAATGAT CTATGCCAGCTCCAAGGATGCCATCAAGAAGAAGCTGACAGGAATCAAGCATGAATTACAAGCTAACTGC TACGAGGAGGTCAAGGACCGCTGCACCCTGGCAGAGAAACTAGGTGGCAGCGCCGTCATTTCCCTGGAGG GCAAGCCTTTGTGAGCCACCTCCAGCCCCCTGCCTGGAGCATCTTAACAGCCCCAGACCTGCTCTTGGGT GTTGCAGGCTGCCCCTTTCCTGCCAGACCGGAGGGGCTGGGGGGATCCCAGCAGGGGGAGGGCTATCCCT TCACCCCAGTTGCCAAACATCCCTCCCACCCCCTGGACCGTCCTTCTCCCTCCATCCCTGACGGTTCTGG CCTTCCCAAACTGCTTTTGATCTTCTGATTCCTCTTGGGTTGACGCAGACCAAGTCCCGTCCTAGGCACC CAGTTTGGGGGGAGCCTGTATTTTTTTTTTTAACGACACCCCTACTCCGTATCCCTCCCCATCCCATGCT GCCAACTTCTAACCACAATAGTGACTCTGTGCTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAA TGACCCTCCCTGCCCCAGCTGGCTGCCCTCCCCTTTCCTTTGATCTTGACCACTCATGGAAGCAGGACCA GTAAGGGACCTTCAATTTAAAACAAAACAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC058726
• Insert Size: 501 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058726, AAH58726
• RefSeq Size: 1096 bp
• RefSeq ORF: 501 bp
• Locus ID: 12631
• Cytogenetics: 19 A
• Gene Summary: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11809832). Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity). Required for neural tube morphogenesis and neural crest cell migration (PubMed:15649475).[UniProtKB/Swiss-Prot Function]