Wac (BC080851) Mouse Untagged Clone

CAT#: MC206098

Wac (untagged) - Mouse WW domain containing adaptor with coiled-coil (cDNA clone MGC:90590 IMAGE:6848738), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wac
• Synonyms: 1110067P07Rik; A230035H12Rik; AI256735; AI256776; Wwp4
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC080851
  GCGAGAAGGACCCGGCGGCGGCGGAGCAGGAGGAGGGAAGGTGCTGGAGGCGTTCGCTCTCCGCGGGGAC CGGAACACGGCCTCTCACCCCCTCCCCCCGGCTCGCGCTGCCGCCCCGTAGTTGGCACCTCTGCCCCAGC CGGCCCTAAGCGGTGGTGACGGAGGGAGATGGCCCGGGACCGCCGGCGCCAGTAACGGGGAGGTGCCGCG AGCCGCCGAGGGTGCGCTGAGACCTGGTGCCGGGGCTGCCCGCCGCCCGCCCGCCGCCCGCGCGCCCGCC CGCCTGCCTCGCGGCCGCTCTCCCCCTCCCCGACACACACACACAGCCGGGCATTGATGGTAATGTATGC GAGGAAACAGCAGAGACTCAGTGATGGCTGTCACGACCGGAGAGGGGACTCTCAGCCTTTCCAGGCACTT AAGTATTCTTCAAAGAGTCACCCCAGTAGTGGTGATCACAGACATGAAAAGATGCGAGACGCCGCAGATC CTTCACCACCAAACAAAATGTTGCGGAGATCTAATAGTCCTGAAAACAAATACAGTGACAGCACAGGTCA CAACAAGGCCAAAAACGTGCACACTCAGAGAGTTAGAGAAAGGGACGGTGGGACCAGTTACTCTCCACAA GAAAACTCACACAACCACAGTGCACTTCATAGTTCAAATTCACATTCTTCTAATCCAAGCAATAATCCAA GCAAAACTTCAGATGCACCTTATGATTCTGCAGATGATTGGTCTGAGCACATTAGCTCATCTGGAAAAAA GTACTACTACAATTGTCGCACAGAAGTTTCACAGTGGGAGAAACCAAAAGAGTGGCTTGAAAGAGAACAG AGACAAAAAGAAGCAAATAAGCTGGCAGTTAATAGTTTCCCAAAAGACAGGGATTACAGAAGAGAGGTGA TGCAAGCAACAGCCACTAGTGGGTTCACCAGTGGAATGGAAGACAAGCATTCCAGCGATGCCAGTAGTTT GCTCCCACAGAATATTTTGTCTCAAACAAGCAGACACAATGACAAAGACTACAGACTGCCAAGAGCAGAG ACTCACAGTAGCTCTACGCCAGTACAGCACCCCATCAAACCAGTGGTTCATCCAACCGCTACCCCAAGCA CTGTTCCTTCTAGTCCATTTACGCTTCAGTCTGATCACCAGCCAAAGAAATCATTTGATGCTAATGGAGC ATCTACTTTATCCAAACTGCCTACACCCACAGCTTCTCTCCCTGCACAGAAAACAGAGAGAAAAGAATCT GCTCCAGGAGACAAATCAATATCACATTCTTGCACAACTCCTTCCACATCTTCAGCCTCTGGCCTGAACC CTACATCTGCGCCTCCGACATCTGCTTCAGCAGTACCTGTTTCTCCCGTTCCACAATCAACAATACCTCC ATTACTTCAGGACCCAAATCTTTTTAGACAGTTGCTTCCTGCTTTGCAAGCCACGCTACAGCTTAATAAT TCTAATGTGGACATATCCAAAATAAATGAAGTTCTTACAGCAGCTGTAACACAAGCTTCCCTGCAGTCTA TCATCCACAAGTTTCTTACTGCTGGACCATCTGCTTTCAACATCACCTCACTGATTTCTCAAGCTGCCCA GCTTTCTACACAAGCCCAGCCATCTAATCAGTCTCCAATGTCTCTGACATCTGATGCTTCATCCCCAAGA TCCTATGTGTCTCCAAGAATAAGCACACCTCAAACTAATACAGTCCCTATGAAACCTTTGATTAGTACTC CACCTGTTTCATCCCAGCCAAAGGTTAGTACTCCAGTAGTTAAGCAAGGACCAGTGTCACATTCGGCTAC ACAGCAGCCTGTAACTGCTGACAAGCAGCAGAGCCATGATTCTGTTTCTCCTCGAAGTCTTCAGCGCTTA AGTAGCCAGAGAAGTCCATCACCTGGTCCAAATCATACTTGTAGTAGTAATGCATCAACTGCAACTGTTG TACCACAGAATGCATCTGCTAGACCTGCGTGCTCATTAACACCTACTCTAGCAGCGCACTTCAATGATAA TCTCATAAAGCATGTTCAAGGGTGGCCTGCAGACCACGCAGAGAAACAGGCATCAAGATTGCGTGAAGAA GCCCATAATATGGGAAGTGTACATATGTCAGAAATATGTACTGAGTTAAAAAATTTGAGATCATTAGTTC GAGTATGTGAAATTCAAGCAACTTTGCGAGAGCAGAGGATACTATTTTTGAGACAACAAATTAAGGAACT CGAAAAGCTGAAAAATCAGAATTCCTTCATGGTTTGAAGATGTGAATAATTGCACATGATCTCTTAGAGG AACTGTAAATCTATTGCCCAGTCATAACATTTTGAGCTGCATTTAAGTAGACTATGGACCGTTAAGCTGG GCAAATGAAATGACAAGGGGACGGGGTCTGTGAGAGTCAATTCAGGGGAAAGATACAAGATTGATTTGTA AAACCCTTGAAATGTAGATTTCTTGTAGATGTATCTTCACGTTGTAAATATGTTTTGTAGAGTGAAGCCA TGGGAAGCCATGTGTAACAGAGCTTAGACATCTGAAACTAATCAATGCTGAGGTGGCTAAATACCTAGCC TTTTACATGGAAACCTGTCTCAAAATTAGCTTTTTTTAAAAAAAGAAAAAGAAAAAATTGGGGGGGGGGG TTAATTTATCATTTAGAAATCTTGCATTTTCAAAAATCCAGTGCAAGCGCCAGGTGATCTGTGTCTGAGG ATACGATTTTGAACCTTATGAGCAGTGTACAAATATCATGAGACAACTGATCAGCACTTGCACCTGATCA AGAGCAGTGCTGCTCCATTTGTTTTGCAAAGATATGTTTTCATTTCCTGTATGTTCCATTCCCTCTGAAG TCCCTTGGCCTGATTTTATCCATTTTTTTTTTTTTTTTAAGGTGCCCCCTTTCCTTTCTTGGGGCACTGT TGCTGTGGCACTTTTCTATAACCTTGTCATTCCTATGTACAGTAGCTGGAAGTCGCAGTGAGCAAGCATA ACCCACGTTTGTATAAATTATTGAAATCCATTTTCACCTAAGAATGGACTTAAGAAGTACTGCTGGGCAA GTGTGCTGAAAGTACATTAACTGCTCAAATACAAGGAAGTGGCCCAATGAACATGGTTGTGGGAGGGGAA AGGAGAAACAAAGCTAGTCAGATGTGAATTGTATCTGTTGTAATAAACATGTTTAAACAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC080851
• Insert Size: 1941 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC080851, AAH80851
• RefSeq Size: 3282 bp
• RefSeq ORF: 1941 bp
• Locus ID: 225131
• Cytogenetics: 18 A1
• Gene Summary: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway.[UniProtKB/Swiss-Prot Function]