Aof2 (BC059885) Mouse Untagged Clone

CAT#: MC206088

Aof2 (untagged) - Mouse amine oxidase (flavin containing) domain 2 (cDNA clone MGC:69806 IMAGE:6815975), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aof2
• Synonyms: 1810043O07Rik; AA408884; Aof2; D4Ertd478e; Kdm1; Lsd1; mKIAA0601
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC059885
  CGCGGGGGTGTTTGGCTTCGCACGGAGCGTGAGAGGTGCGGGGCGGAGAGGCGCGAGGCGGCTGCGGACC CACGGAGCGGCAGACCGATCGGCCCCTGCGGCCCGCGGCGGCCAGGCGGCCCGAGATGTTGTCTGGGAAG AAGGCGGCGGCGGCGGCAGCGGCAGCGGCGGCGGCGGCGGCTGCTGGGACCGAGGCCGGGTCCGGGGCGG CGGGCGGTGCCGAGAACGGCTCTGAGGTGGCCGCGCCGCCCGCGGGCCTGACGGGCCCCACCGACATGGC TACGGGGGCGGCGGGCGAGCGCACTCCCCGAAAGAAGGAGCCTCCGCGGGCCTCGCCGCCCGGGGGCCTA GCCGAGCCGCCGGGGTCTGCTGGGCCCCAGGCGGGGCCCACAGCCGGGCCCGGCTCCGCGACGCCCATGG AGACCGGAATAGCCGAGACCCCGGAGGGCCGACGGACCAGCCGGCGCAAGCGGGCCAAGGTAGAATACAG AGAAATGGATGAAAGCTTGGCCAACCTCTCAGAAGATGAATATTATTCGGAAGAAGAAAGAAATGCTAAA GCAGAGAAGGAAAAGAAGCTTCCCCCACCACCTCCTCAAGCCCCACCTGAGGAAGAAAATGAAAGTGAGC CGGAAGAGCCGTCTGGTGTGGAGGGTGCAGCTTTTCAAAGCCGACTTCCCCATGACCGAATGACCTCTCA GGAAGCAGCCTGTTTCCCAGACATCATCAGTGGGCCTCAGCAGACACAGAAGGTTTTTCTGTTCATCAGG AATCGCACATTGCAGTTATGGCTGGACAACTCAAAGATCCAGCTGACGTTTGAAGCCACTCTCCAGCAGC TGGAAGCGCCTTACAACAGCGATACTGTGCTTGTCCACCGAGTTCACAGTTACTTAGAGCGCCATGGTCT TATCAACTTCGGCATCTACAAGAGGATAAAACCCTTACCAATTAAAAAGACAGGAAAGGTGATTATTATA GGTTCAGGTGTTTCTGGCTTGGCAGCAGCTCGACAGCTACAGAGTTTTGGGATGGATGTCACACTTCTGG AAGCCAGGGATCGAGTAGGTGGACGAGTTGCTACATTTCGAAAAGGAAACTATGTAGCTGATCTTGGCGC CATGGTTGTAACAGGTCTTGGAGGGAATCCCATGGCTGTCGTCAGCAAACAAGTAAATATGGAACTGGCC AAGATCAAGCAAAAATGCCCACTTTATGAAGCCAATGGACAAGCTGTTCCAAAAGAAAAAGATGAAATGG TAGAACAAGAATTTAACCGGTTGCTAGAAGCCACTTCTTACCTTAGTCACCAGTTAGACTTCAACGTCCT CAATAATAAACCTGTATCCCTTGGCCAGGCATTGGAGGTTGTCATTCAGCTGCAAGAAAAGCATGTCAAA GATGAGCAGATTGAACATTGGAAGAAGATAGTGAAAACTCAGGAGGAGTTGAAAGAGCTTCTTAATAAGA TGGTAAATTTGAAGGAGAAAATTAAAGAGCTCCATCAGCAATACAAAGAAGCTTCAGAAGTGAAGCCGCC CAGAGATATCACAGCCGAGTTCCTGGTGAAGAGCAAGCACAGGGACCTGACTGCCCTCTGCAAGGAATAT GATGAATTAGCTGAAACACAAGGAAAGCTAGAAGAAAAACTTCAAGAATTGGAAGCCAATCCCCCAAGTG ATGTATACCTCTCATCAAGAGACAGACAAATACTTGACTGGCATTTTGCAAATCTTGAATTTGCCAACGC CACACCTCTCTCTACCCTCTCTCTTAAACATTGGGATCAGGATGATGACTTTGAGTTTACTGGAAGCCAC CTGACAGTAAGGAATGGCTACTCATGTGTGCCTGTGGCTTTAGCTGAAGGCTTGGACATTAAACTGAACA CAGCAGTGCGGCAGGTTCGCTACACAGCCTCAGGATGTGAAGTGATTGCTGTGAACACACGTTCCACAAG TCAAACCTTTATTTATAAGTGTGATGCAGTTCTCTGTACACTTCCTTTGGGAGTGTTGAAGCAGCAGCCA CCAGCTGTTCAGTTTGTGCCACCTCTTCCTGAGTGGAAAACATCTGCAGTCCAAAGGATGGGATTTGGCA ACCTTAACAAGGTGGTGTTATGCTTTGACCGTGTGTTCTGGGACCCAAGTGTCAATTTGTTTGGGCACGT TGGCAGTACAACTGCTAGCAGGGGTGAGCTCTTCCTCTTCTGGAACCTATATAAAGCTCCAATACTATTG GCCCTGGTAGCAGGAGAAGCTGCTGGCATTATGGAGAACATTAGTGATGATGTGATTGTCGGCCGGTGCC TGGCCATTCTCAAAGGGATTTTTGGCAGCAGTGCAGTCCCACAGCCCAAGGAAACTGTGGTATCTCGTTG GCGTGCTGATCCGTGGGCCCGGGGCTCCTATTCTTATGTGGCTGCAGGATCCTCTGGAAATGACTATGAT TTAATGGCTCAGCCGATCACTCCTGGCCCCTCAATTCCAGGTGCCCCACAGCCAATCCCAAGACTCTTCT TTGCTGGAGAACACACAATCCGGAACTACCCAGCTACAGTCCATGGTGCTCTGTTGAGTGGGCTTCGAGA AGCAGGAAGGATTGCCGACCAGTTTTTGGGAGCCATGTACACTTTGCCTCGTCAGGCCACACCAGGTGTC CCTGCACAGCAGTCCCCAAGTATGTGAGACAGATGGTTCTGAACAGAGAGATCCAACGGCATGTCATCTG CCACGTAAGCAAGCTCTTCTAGCAATACTAGATCCTACTGAGAAACTCCATGTCATCAGCTACTGGGACT CCTAGTTTGACAGCAGAGGCTGGCTCCTTTGGCTGACAGCAACTTACCCATTGATTTGGAAGTACAGCTC CATAAAGACTGCTCGAGAAGCAAGTGGTGTGAGATAACCTCTTAGTCTATGGTGTTTGTTTGTTTTTGTT TTTTTTTAATATATTTTGAGAATAAAACTTTAAAATAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC059885
• Insert Size: 2412 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059885, AAH59885
• RefSeq Size: 2999 bp
• RefSeq ORF: 2412 bp
• Locus ID: 99982
• Cytogenetics: 4 68.8 cM
• Gene Summary: Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation (By similarity). Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.[UniProtKB/Swiss-Prot Function]