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Gucy1a3 (BC057327) Mouse Untagged Clone

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SKU
MC206075
Gucy1a3 (untagged) - Mouse guanylate cyclase 1, soluble, alpha 3 (cDNA clone MGC:66907 IMAGE:6825960), (10ug)
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gucy1a3
Synonyms 1200016O07Rik; sGC-alpha1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC057327
GTGCCTGCCAGCCGGACCGGACCCCAAGGCGAAGAGCAGCAGTGCACAGCCTGGGGAGCCAGCGGAGCAA AGACACCTTTGGCCCGATGCCCCTGGCCTCCTGTGATCGCATCATGGTGCTGGGTCACTCCTGTCCTTGA GTCAGTAGAAGCAGATCTTCATCAGTCCACATCAACACCAGCTAGTCAGAAGGAAGCCACTGCCAAGCTC CAGGAACACCATGTTCTGCAGGAAGTTCAAGGATCTCAAGATCACAGGGGAGTGTCCTTTCTCCTTACTG GCCCCTGGTCAGGTTCCTAAGGAGCCAACAGAGGAGGTGGCTGGAGGCTCTGAGGGCTGCCAGGCTACTC TGCCCATCTGCCAGTACTTTCCTGAGAAGAATGCAGAAGGGAGTCTCCCCCAAAGAAAGACAAGCCGCAA CAGAGTCTACCTGCACACCCTGGCAGAGAGTATTTGCAAGCTCATCTTCCCAGAGTGTGAGCGACTGAAC CTTGCACTTCAGAGAACCTTGGCAAAACATAAAATAGAAGAAAACAGGAAATCTTCAGAAAAAGAAGACC TTGAAAAAATAATCGCAGAAGAAGCAATTGCAGCAGGTGCCCCAGTGGAGGCGCTCAAAGACTCTCTGGG CGAGGAGCTGTTCAAGATCTGCTATGAGGAAGATGAGCACATTTTGGGCGTGGTTGGCGGCACCCTGAAG GACTTTCTAAATAGCTTCAGCACGCTCCTCAAGCAGAGCAGCCACTGCCAAGAGGCGGAGAGGCGGGGAC GACTGGAAGATGCCTCCATCTTATGCCTGGACAAGGACCAGGACTTTCTAAATGTTTACTACTTCTTCCC GAAGAGAACCACAGCCCTGCTTCTCCCTGGTATCATTAAAGCGGCTGCTCGCATACTGTACGAAAGCCAC GTGGAGGTGTCCCTGATGCCTCCCTGCTTCCGAAGTGACTGTACCGAGTTTGTGAACCAGCCCTATTTGC TCTACTCCGTTCATGTGAAGAGCACCAAGCCGTCCCTGTCCCCAGGCAAGCCCCAGTCCTCGCTAGTGAT CCCCGCTTCGCTCTTCTGCAAGACTTTCCCGTTCCATTTCATGCTGGACCGAGACCTGGCCATCCTGCAG CTGGGTAACGGCATCAGAAGGCTGGTGAACAAGAGGGACTTCCAAGGGAAGCCCAACTTTGAAGAGTTCT TTGAAATTCTAACTCCCAAAATCAACCAGACATTTAGTGGCATCATGACAATGTTGAACATGCAGTTTGT CATCCGGGTGAGGAGATGGGATAACTCGGTGAAGAAATCGTCAAGGGTTATGGATCTCAAAGGTCAAATG ATCTACATCGTTGAATCCAGTGCCATCTTGTTCTTAGGGTCACCATGTGTGGACAGGCTGGAAGATTTCA CAGGACGGGGGCTCTATCTGTCCGACATCCCAATTCATAACGCCCTGAGGGATGTTGTCTTGATAGGGGA GCAGGCACGGGCTCAAGATGGCCTCAAGAAGAGGTTGGGGAAGCTGAAGGCAACCCTGGAGCATGCCCAC CAAGCCCTGGAGGAGGAGAAGAAGAGGACAGTGGATCTGCTGTGCTCTATCTTCCCCTCTGAGGTTGCTC AGCAGCTGTGGCAAGGACAAATTGTGCAAGCCAAGAAATTCAGCGAGGTCACCATGCTTTTCTCAGATAT CGTAGGGTTCACTGCTATCTGCTCTCAGTGTTCACCTCTGCAGGTCATCACGATGCTCAACGCTCTCTAC ACTCGCTTTGACCAGCAGTGTGGAGAGCTGGATGTCTACAAGGTGGAGACCATCGGGGATGCATATTGTG TGGCAGGTGGATTGCACAGAGAGAGTGACACCCATGCTGTCCAGATAGCACTGATGGCCCTGAAGATGAT GGAGCTCTCCAATGAGGTCATGTCTCCCCACGGAGAACCTATCAAGATGCGAATTGGACTACATTCTGGA TCAGTGTTTGCTGGAGTTGTCGGAGTGAAGATGCCCCGGTATTGCCTGTTTGGAAACAATGTCACTCTGG CTAACAAATTTGAATCCTGCAGTGTGCCTCGGAAAATCAATGTCAGCCCCACCACATACAGGTTACTCAA AGACTGTCCTGGCTTTGTGTTCACCCCGAGATCAAGGGAGGAGCTTCCACCAAACTTCCCTAGTGACATT CCTGGGATCTGTCACTTTCTGGATGCTTATCACCATCAAGGACCTAATTCCAAACCATGGTTCCAGGATA AAGATGTGGAAGATGGAAACGCCAACTTCTTAGGCAAAGCGTCAGGGATAGATTAGTGAGCCACATGCTC TTATGTTTGATGCCTTTGAAGGTGTGCAGAACCTCTGTGTTGACCTTAGGATTACAGAAGATAAAAAAAA TCAGTGTTAAAATTACAGAGGCTAAACACAGGTTTCCTCTGTCTCCCATTTAAGATGGAAAAGAAAAGTG TTCACTTCAGCGCTTCAACCTTCTTCTATTTAAGAAACAGACCTCAAAAGTGACCTGTGACTTTTGGGGG CATAGTTGTGTTCTAGAACAGTCACTACCTGCACTCAAAATCCAGCACATTGTACATATTCCAGGCAACT GTAGTCAAGTTTAAGTGTTGAAGTAATGGGTTCACCTATGGCCTGTGGCCAGCACAAGCTGTGGTTATTA AAGTGTATGTGTGTGTGATAGTGTCGCCTGTAAAATCTCTGAATAGAAACAACAGTGTTTAGTTCTGCAA GAACTCCCAGCCTCTCGACTCTACATTCAATTAGATTTCCTTATTTAATCGTGTCATATGTAGCACAATA GACCAGCATCACCTTTCCAGCTACATCTTTGTGCCTATGTATGTGATTTCTTTTCCTTTTTTTTAAAAGA AAATAAGCAATTAGACATTAGATGAAATGTGATATAAGATAGTTCTACAAGTATCAAAGAATATTCTCAT GTTTTGTCATTTAAAATATCCTCTATAGCACACTATATAATACAGCAAATACTTTTCTTTTTGATGTTAT ATGTAATAAAACATTTTAATTAATCGTAACAACAATGTACTTCCTACTAATCCACGTAAAATGTACGAAT AGCAAAACTATCAGAATACAAAGACTTATAGTAACCCTAGTTAAAATGCAATAATATTCAAATTTAATAA AGTCATAGAGTTAACATTTGCATCCAGAGAGCCTGCTGTAGAGGACAGTGGTAAACTATTCACAAACATA CATGTATAAATGATCCTCTACTGCCATGTTGAGCCATAAAATCCAAGGGGAAACGTTTTAACTCAGAAGT AGAAGATGAGGCTCCATTTTGTCTTTTTTGATAGCTTCTTAAGTATTTTTACTGACCATTTTAGTGTATG TGTATGTATTAGGAATCTAATTTTGTTATTATTCAGTGTACGCCTGTGAAGTGTTATTCTAGTTAAAATA TTCTAATATTTTCTACAGGAACCACTAGTATCAATGAAGAAAGCTCCCCCCCCCCCATGGTTTCATTGTG GAGAACTCTAAACTTTTTTTTTTTTCTGCTGGAGTTTGAACTAATATCCTTTCCCTCCCATGCCCTCCTC TCAAGTTCCCTTTCTTCCCTTCCCATTGTGCATGTATGTATGATTCTGTGAGTGGAACACAGGGACTGTG CATGCCTGGTAAGGGCTTTTTCCCCATGAAGCTTGGATTGTATTGCTAACTGTGACAGTTTATACTCCCT CCCAAGTGAGCTCCAGTGTGAATTCTGTAAAAGGATGTGGCTTTAAAACAAGACCAGCTTCAAAGCCAAG AGAGATTGACACAGTTGAGTATTTGAATCAGTATTAGCAGAACTATAGACACGCTGACTTTCTATGTGTA TATTTGAAAAGAACTTTAGTTTAAAAGTCCTTTTCTACCTCCATTAGTCTCCAAGCAGTCATCATTACCT AGCTCTAGTTTTTCCAAGGGATACGACTAAACTATTCCAGTGGATTTCTTTAAAGGCTGTTTAAAGGCTC ATTAAACAGTCGCACTGATGTTGGAGAGTATCACAGGCCTTGCGAAGGTCTATTTATGATGTCACAATGA TGTGAAGTTCAATGAAAATCTATGAAGACAGTAAAGAGGTAAATACCCTCTCTTTCTGGAACTAAAATTT AATACAGGGGAACTAATAGGAATCACAGACAATTAGAAAGCACAAAACAGGATGTCATCCTCTTAAGCTG TGCAGTTAGGTAGTTGCTACATTAGTAATAATCTGATTCAGATGCTTGCACAGTAAATAGAATTAATTCC CCACCGTCTTTTAGAGATGGAGATAGAGCTTAGTGACAAAGCACTTGTCTAGCATTTAGGCAGTCCTTAG TTCTAACCCAAGCGGTGTGAAGGAACTATATGCATCTCTCATGAGCATATGATGTATGATCGCACTCACT TGTGTCGCCTGTGTACACACCTGGGGCAAATGGAGGAACTTTATCTAATTCAAGTCAAAAGAAGTATAGA AGCTCCATGTACGGTTCCTATAACTTTTCTATTATTTTGTCATTTTATCTAAATAAAAACATTATCAAGC ATTAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN BC057327
Insert Size 2076 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC057327, AAH57327
RefSeq Size 4578 bp
RefSeq ORF 2076 bp
Locus ID 60596
Cytogenetics 3 E3
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