Flrt3 (BC052043) Mouse Untagged Clone

CAT#: MC206025

Flrt3 (untagged) - Mouse fibronectin leucine rich transmembrane protein 3 (cDNA clone MGC:62414 IMAGE:5702881), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Flrt3
• Synonyms: 5530600M07Rik; C430047I10Rik; mKIAA1469
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052043
  CGCGCAGTCAACTGGATGCTCTCGCGCGCAGTCCTCAGCGCAGGAGTGATCTGAAGCAGGACCGCTCAGG CTGCAGCTGCCGTGGCTTTGTGTGCACTGGATGCCGCGCTCCGGAGACGGGGGAGGGTTTTTACTTCAAC TCACAGGCAATGGAATTACAGCTGTAGCAGCAGGGTATAAAGGATTGCTTTTTCCTCGTCTTCCTGGAGG TGCTCAGTCCTGGCCCATTTTAAGGACGAGAAATATAAAGGGAATTTAGTGTGCTTCCTTCTCTTTCCAT GAAGACTGCATGCACTGTGCCCTTTGCTTCTTAAAAGAGACTCCACCCACTCCAGTAGACCGGGGACTAA AACAGAAATTCTGAGAAAGCAGCAAGAAGCAGAAGAAATAGCTATTTCACAGCAGTAACAGAAGCTACCT GCTATAATAAAGACCTCAACACTGCTGACCATGATCAGCCCAGCCTGGAGCCTCTTCCTCATCGGGACTA AAATTGGGCTGTTCTTCCAAGTGGCACCTCTGTCAGTTGTGGCTAAATCCTGTCCATCTGTATGTCGCTG TGACGCAGGCTTCATTTACTGTAACGATCGCTCTCTGACATCCATTCCAGTGGGAATTCCGGAGGATGCT ACAACACTCTACCTTCAGAACAACCAAATAAACAATGTTGGGATTCCTTCCGATTTGAAGAACTTGCTGA AAGTACAAAGAATATACCTATACCACAACAGTTTAGATGAATTCCCTACCAACCTTCCAAAGTATGTCAA AGAGTTACATTTGCAAGAGAATAACATAAGGACTATCACCTATGATTCACTTTCGAAAATTCCGTATCTG GAAGAGTTACACTTGGATGATAACTCAGTCTCGGCTGTTAGCATCGAAGAGGGAGCATTTCGAGACAGTA ACTATCTGCGGCTGCTTTTTCTGTCCCGTAACCACCTTAGCACAATCCCGGGGGGCTTGCCCAGGACTAT TGAGGAATTACGCCTGGATGACAATCGCATATCAACGATCTCTTCCCCATCACTTCATGGTCTCACAAGC CTGAAACGCCTGGTTTTAGATGGAAACTTGTTGAACAACCATGGTTTGGGTGACAAAGTTTTCTTCAACT TAGTAAACTTAACAGAGCTGTCCCTGGTGAGGAATTCCTTGACAGCAGCGCCAGTGAACCTTCCCGGCAC AAGCCTGAGGAAGCTTTACCTTCAAGACAACCATATCAACCGGGTACCCCCAAATGCTTTTTCTTATTTA AGGCAGCTGTATCGACTCGATATGTCTAATAATAACCTAAGCAATTTACCTCAGGGTATCTTTGATGATT TGGACAATATAACCCAACTGATTCTTCGCAACAATCCTTGGTATTGTGGATGCAAGATGAAATGGGTACG AGACTGGTTACAGTCGCTACCGGTGAAGGTCAATGTGCGTGGGCTCATGTGCCAAGCCCCAGAAAAGGTC CGTGGAATGGCTATCAAGGACCTCAGTGCAGAACTGTTTGATTGTAAAGACAGTGGGATTGTGAGCACCA TTCAGATAACCACTGCAATACCCAACACAGCATATCCTGCTCAAGGACAGTGGCCAGCTCCTGTGACCAA ACAACCAGATATTAAAAACCCCAAGCTCATTAAGGATCAGCGAACTACAGGCAGCCCCTCACGGAAAACA ATTTTAATTACTGTGAAATCTGTCACCCCTGACACAATCCACATATCCTGGAGACTTGCTCTGCCTATGA CTGCTCTGCGACTCAGCTGGCTTAAACTGGGCCATAGCCCAGCCTTTGGATCTATAACAGAAACAATCGT AACAGGAGAACGCAGTGAATACTTGGTCACCGCCCTAGAACCTGAATCACCCTATAGAGTATGCATGGTT CCCATGGAAACCAGTAACCTTTACCTGTTTGATGAAACACCTGTTTGTATTGAGACCCAAACTGCCCCTC TTCGAATGTACAACCCCACAACCACCCTCAATCGAGAGCAAGAGAAAGAACCTTACAAAAATCCAAATTT ACCTTTGGCTGCCATCATTGGTGGGGCTGTGGCCCTGGTAAGCATCGCCCTCCTTGCTTTGGTGTGTTGG TATGTGCATAGGAACGGGTCACTGTTTTCACGGAACTGTGCGTACAGCAAAGGGCGGAGGAGAAAGGATG ACTATGCAGAAGCCGGTACTAAGAAAGACAACTCCATCCTGGAAATCAGGGAAACTTCTTTCCAGATGCT ACCGATAAGCAATGAACCCATCTCCAAGGAGGAGTTTGTAATACACACCATATTTCCTCCGAATGGGATG AATCTGTACAAGAACAACCTCAGTGAGAGCAGTAGTAACCGGAGCTACAGAGACAGTGGCATCCCAGACT CGGACCACTCACACTCATGATGCAAGGAGGTCCCACACCAGACTGTTCCGGGTTTTTTTTTAAAAAACCT AAGAAAGGTGATGGTAGGAACTCTGTTCTACTGCAAAACACTGGAAAAGAGACTGAGAGAAGCAATGTAC TGTACATTTGCCATATAATTTATATTTAAGAACTTTTTATTAAAAGTTTCAGATTTCAGGTTGCTGCTGC GGTTGATGTAGTGGGGATGCCTGAACACAATTCTATATTTTAGTATTTTTTAGTAATTTGTACTGTATTT TCCTTGCAGATATTGAAGTTATAAACCATTTACTTTGTGTTCTACTGAGTAAGATGACTTGTTGACTGTG AAAGTGAATTTTCCCGCTGTGTTGAACAATCAGGACTGCGTTCACATGAGACCCTTGTAGTATAAGCACA GGCCGTTTTTCACTTTGGTATTAATACAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC052043
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052043, AAH52043
• RefSeq Size: 2868 bp
• RefSeq ORF: 1950 bp
• Locus ID: 71436
• Cytogenetics: 2 F3
• Gene Summary: Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners (PubMed:19056886, PubMed:25374360). Plays a role in the spatial organization of brain neurons (PubMed:25374360). Plays a role in vascular development in the retina (PubMed:25374360). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:22405201, PubMed:25374360). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25374360). Promotes neurite outgrowth (in vitro) (By similarity). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length (By similarity). Plays a role in the regulation of the density of glutamaergic synapses (PubMed:22405201). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning (PubMed:19056886). Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development (PubMed:18448090). Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development (PubMed:19056886).[UniProtKB/Swiss-Prot Function]