Ctu2 (NM_153775) Mouse Untagged Clone

CAT#: MC206007

Ctu2 (untagged) - Mouse cytosolic thiouridylase subunit 2 homolog (S. pombe) (Ctu2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctu2
• Synonyms: 2310061F22Rik; C16orf84; Ncs2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC036332
  GCTCCTCACGCCACTCCGCAGTCGGGACCCACCCCTCCATGTGTCAGGCCGGTGAGGATTACGCGGGGCC GGCGCGGCGGGAGCCTCCGCCCGTGCCGCGGCCAAGCCGGGAGCAGAAATGTGTGAAGTGCGCAGAAGGC CTGCCGGTGGTGGTGATACGAGCGGGAGATGCCTTCTGCAGGGTTTGTTTCAAGGCATTCTACGTTCACA AATTCAGAGCCATGCTTGGGAAGAACCGGGTCATCTTTCCTGGGGAGAAGGTGCTCCTGTCCTGGTCTGG GGGCCCTTCGTCCAGCTCCATGGTCTGGCAAGTGCTTGAGGGCCTGAGTCAGGATTCTGCGAAACGGCTA CGCTTTGTGCCAGGAGTCATCTATGTTGATGAGGGCGCAGCCTGTGGTCAGAGCTTGGAGGACAGACAGA AGACGGTGGCTGAGGTGAAGCGGATCCTGGAGAACACTGGTTTCCCATGGCATGTGGTTGCCTTAGAAGA GGTGTTCAGCTTGCCCCCATCAGTGCTATGCTGCACTTCCCAGGAGTCAGCAGGGACAGAGGAGGCCTAC AAGGCTGCCGTGGACCGCTTCCTGCAGCAGCAGCAGCAGCAGCAGCAGCGTGTGCTGGGAGCTGAGGCCG GTGCCAGCCCAGCTCAGGGAGAGGCTCGGCTGCATCCATCCCACGGCCGGGAACCCTCAGGCACGGCCGG GTACCCCACAGCTGCTCAGACAGAGGCCTTGTCTAGGTTGTTCAGCTCCATAAAGACACTGACAGCCAAA GAGGAGCTTCTGCAGACCCTGAGGACCCATCTGATTGTGCACATAGCCCGAGTCCATGGCTATTGCAAGG TAATGACAGGGGAGACCTGCACCCGCCTGGCCATCAAGCTTATGACCAACCTGGCCCTGGGGAGAGGCGC CTTCCTCGCCTGGGACACGGGCTTCTCAGATGAGCGACACGGGGATGTGGTGTTGGTGAGGCCCATGCGG GACCACACGCTGAAGGAAGTGGCCTTCTACAACCACCTGTTTAGAGTCCCCTCAGTCTTCACACCAGCCA TCGATACCAAGGCTCCAGAAAAGGCTAGCATCCACCGGCTGATGGAGGCCTTTATCCTGAGGCTACAGAC TCTGTTCCCGTCTACAGTCAGCACCGTGTACAGGACGAGTGAGAAGCTGGTCAAAGCTCCCCGGGAGGGT TGTGCCGCTGGCCCGTCAGGCCCCAGCTGCCTCCTGTGCATGTGTGCCCTGGACATTGACACTGCTGACA GTGCCACGGCCTTTGGGGCTCAGTCCTCCTCACATCTCTCCCAGATGCCGAGTGCTGAGGCTGGGATGCC TACCCAGCCCTGCTGTGCTGCAGGGGAGGGTCAGGCCCAGAGCTGCCATAGGGAAGTTGGCAAGAGGGGG GATGCACGGGCCTGCATCACAGAGCAGTTATGTTATAGCTGCCGAGTGAACATGAAGGACCTGCCCTCCT TGGACCCATTGCCACCCTACGTCCTTGCTGAGGCTCAGCTCCGCAGCCAGAGGGGCTCGGTCTCTGAGGA AATCCAGGAGTATCTGATTACAGATGAGGAGGAAGACAGCTGAGTCGTGCGGAGCCATGAAGCAGGAAGG CGAGGACAAAAGGATTGGTCTGTGACAGTCCGTTTGTATAAATAAAAATGTTTTACTTAGAAAAAAAAAA AAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_153775
• Insert Size: 1284 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC036332, AAH36332
• RefSeq Size: 1685 bp
• RefSeq ORF: 1284 bp
• Locus ID: 66965
• UniProt ID: Q3U308
• Cytogenetics: 8 E1
• Gene Summary: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.[UniProtKB/Swiss-Prot Function]