Epha1 (NM_023580) Mouse Untagged Clone

CAT#: MC205994

Epha1 (untagged) - Mouse Eph receptor A1 (Epha1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epha1
• Synonyms: 5730453L17Rik; AL033318; Eph; Esk
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC071215
  GTCCCAGGTCCCGGCCGGCGCCATGGAGCGGCGCTGGCCCCTGGGGCTTGCATTGCTGCTGCTGCTGCTC TGCGCCCCGCTGCCCCCGGGGGCGCGCGCCGAGGAAGTCACTCTAATGGACACAAGCACAGCACAAGCAG AGCTGGGCTGGCTTCTGGATCCCCCAGAGACTGGGTGGAGTGAGGTGCAACAAATGCTAAACGGGACACC CCTGTACATGTACCAAGACTGCCCAATACAGGAAGGTGGGGACACTGACCACTGGCTTCGCTCCAATTGG ATCTACCGCGGAGAGGAAGCTTCACGCATCTACGTGGAGCTGCAGTTCACCGTGCGGGACTGTAAGAGTT TCCCAGGGGGAGCTGGGCCTCTGGGATGCAAAGAGACCTTCAACCTTTTCTACATGGAGAGTGACCAGGA TGTGGGCATTCAGCTCCGACGACCTTTGTTCCAAAAGGTAACAACTGTGGCAGCAGACCAGAGCTTCACC ATCAGAGACCTGGCATCTGGCTCTGTAAAGCTGAATGTAGAACGCTGCTCGTTGGGCCACCTCACCCGCC GTGGCCTCTACTTAGCTTTCCACAACCCGGGTTCCTGTGTGGCGCTAGTGTCTGTAAGGGTGTTCTACCA GCGCTGTGCCGAGACCGTGCATGGCTTGGCCCACTTCCCTGACACTCTCCCTGGACCTGGAGGGTTGGTT GAAGTAGCTGGAACGTGCCTCTCCCATGCACAGATCAGCTTGGGGTCCTCAGGTACACCACGAATGCACT GCAGCCCTGATGGCGAGTGGCTGGTGCCTGTGGGTCAGTGCCAGTGCGAGCCTGGCTATGAAGAAAGCAG TGGAAATGTGGGATGCACTGCCTGTCCTACTGGTTTCTATCGAGTGGACATGAATACACTCCGTTGTCTC AAGTGCCCCCAACATAGCATAGCAGAGTCTGAGGGGTCTACCATCTGTACCTGTGAGAATGGACATTATC GAGCCCCTGGGGAGGGTCCCCAGGTAGCATGCACACGTCCCCCATCGGCTCCCCAAAATCTGAGCTTCTC CACATCAGGGACTCAACTCTCCCTGCGCTGGGAGCCCCCCAGAGATACAGGGGGACGCCATGATATCAGA TACAGCGTGGAGTGCTTGCAGTGTCGGGGCATTGCACAGGATGGGGGTCCCTGCCAACCCTGTGGAAAAG GTGTGCACTTTTCCCCGGCTGCTTCCGGGCTCACCACATCTACCGTGCAAGTGCAAGGCCTCGAGCCTTA CGCCAACTACACATTTACCGTCAAATCCCAAAACAGAGTGTCAGGACTGGACAGTTCCAGCCCTAGCAGC GCCTCCCTCAGTATCAACATGGGGCACGCAGAGTCACTCTCTGGCCTGTCACTGAAGCTGGTGAAGAAAG AACCGAGGCAGCTGGAGCTGACTTGGGCAGGGTCCCGACCCCGAAATCCTGGAGGGAATCTGAGCTATGA GCTGCACGTGCTGAATCAGGACGAAGAATGGCACCAGATGGTGTTGGAACCCAGGGTCTTGCTGACAAAA CTTCAGCCAGATACCACATACATTGTCAGAGTGCGAACACTGACCCCACTGGGGCCTGGCCCTTTCTCCC CTGACCATGAGTTTCGGACAAGCCCACCAGTTTCCAGAAGCCTGACCGGAGGAGAGATTGTGGCCGTCAT CTTTGGATTGCTGCTTGGAATAGCTCTGCTGATCGGGATTTATGTCTTCCGTTCAAGGAGAGGCCAGAGA CAGAGACAGCAGAGGCAGCGTGAACGCACCACCAATGTCGATCGAGAGGACAAGCTGTGGCTAAAACCCT ATGTGGACCTCCAGGCCTATGAGGACCCTGCACAGGGAGCCTTAGACTTTGCCCAGGAACTGGACCCAGC CTGGCTGATTGTGGACACTGTCATAGGAGAAGGGGAGTTTGGTGAAGTGTATCGGGGAGCCCTGAGACTC CCCAGCCAAGATTGCAAGACTGTGGCCATTAAGACCTTGAAAGATACATCCCCAGATGGCTACTGGTGGA ATTTCCTTCGAGAGGCAACTATCATGGGCCAGTTCAACCACCCACACATTCTACGCCTAGAAGGTGTCAT CACAAAAAGAAAGCCCATCATGATCATCACAGAATTTATGGAAAATGGAGCCCTGGATGCCTTTCTGAAG GAACGGGAGGACCAACTAGCTCCTGGTCAGCTAGTGGCTATGCTACTGGGCATAGCATCAGGCATGAACT GCCTCAGTGGCCACAATTATGTCCATAGAGACCTGGCTGCCAGGAACATCTTGGTGAATCAGAACCTGTG CTGCAAGGTGTCTGACTTTGGCTTGACCCGCCTCCTGGATGACTTTGACGGCACCTATGAAACCCAGGGA GGAAAGATCCCCATCCGATGGACAGCCCCAGAAGCTATTGCCCATCGGATCTTCACCACAGCCAGTGATG TGTGGAGCTTTGGGATTGTAATGTGGGAGGTGTTGAGTTTTGGCGACAAACCCTATGGGGAGATGAGCAA CCAAGAGGTAATGAAAAGCATTGAAGATGGGTACCGGTTGCCCCCTCCTGTGGACTGTCCTGCCCCTCTC TATGAACTCATGAAGAACTGCTGGGCTTACGATCGTGCCCGTCGACCCCACTTCCTCCAGCTGCAGGCAC ATCTGGAACAGTTGCTTACTGACCCCCATTCCCTAAGGACAATTGCCAACTTTGACCCTAGGGTGACCTT ACGCCTGCCCAGCCTGAGTGGCTCTGATGGGATCCCTTATCGAAGTGTCTCTGAGTGGCTTGAATCCATA CGCATGAAGCGCTACATCCTGCACTTCCGTTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGA CGGCTGAGGACCTGACGCAGATGGGAATAACGTTGCCAGGGCACCAGAAACGAATTCTCTGCAGTATTCA AGGATTTAAGGACTGAGCATCCACTGAAAAGATGCTCCAGCCCTCTGCCTGCCTCCATTAGCAAGGACGG GGCTACAGTCAACTCCCTGGGCCTTTCCTCAGCCTACGAAATGTAGGCTATTGGTGCTGCTCCTGCCCAG TCAATCAGAACTCTGCCTTTGAACCAAGGAGCCTTTGTTTATAAAGGGGGTGGATGGGTACAAGTGAAGG GGACTGTGGGTGGGTTCTGGGGGAGGGTTTAATATATACACTTACATATGCATTATCTATTTTTGTAAAT AAACAAGAGTTGAGTTTTCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023580
• Insert Size: 2934 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC071215, AAH71215
• RefSeq Size: 3254 bp
• RefSeq ORF: 2934 bp
• Locus ID: 13835
• UniProt ID: Q60750
• Cytogenetics: 6 B2.1
• Gene Summary: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.[UniProtKB/Swiss-Prot Function]